To conduct a retrospective observational cohort study to estimate the abortion complication rate, including those diagnosed or treated at emergency departments (EDs).Using 2009-2010 data among women covered by fee-for-service California Medicaid program and all subsequent health care for 6 weeks after having an abortion, we analyzed reasons ED visits estimated abortion-related rate adjusted relative risk. Complications were defined as receiving diagnosis treatment any source of within...

In the light of accumulating evidence for occurrence spontaneous cortical spreading depression and peri-infarct depolarizations in human brain injured by trauma or aneurysmal subarachnoid haemorrhage, we used DC electrode recording laser speckle imaging to study relationship between depolarization events perfusion ischaemic, gyrencephalic brain. 14 adult male cats anaesthetized with chloralose, one cerebral hemisphere was exposed middle artery occluded. Surface core penumbral territories...

von Willebrand factor (vWF) is a multimeric plasma glycoprotein with three tandem A domains. Domains A1 and A3 bind to platelet Ibα (GPIbα) collagen, respectively. Domain A2 contains the Tyr-1605–Met-1606 bond that cleaved by metalloprotease ADAMTS13, this reaction inhibits thrombus growth. Fluid shear stress increases rate of cleavage, suggesting productive interaction ADAMTS13 requires conformational changes within or near domain A2. The influence adjacent domains was assessed mutagenesis...

ADAMTS13, a metalloprotease, cleaves von Willebrand factor (VWF) in plasma to generate smaller, less thrombogenic fragments. The interaction of with specific ADAMTS13 domains was characterized binding assay employing immobilized on plastic surface. saturable and reversible. Equilibrium occurred within 2 h the half-time for dissociation ∼4 h. Binding similar either recombinant or normal ADAMTS13; from patient who lacked activity showed no binding. stoichiometry one per two monomers, Kd 14 nm....

Von Willebrand factor (VWF) is a multimeric protein that mediates platelet adhesion at sites of vascular injury, and ADAMTS13 (a disintegrin metalloprotease with thrombospondin)is multidomain limits by feedback mechanism in which fluid shear stress induces proteolysis VWF prevents disseminated microvascular thrombosis. Cleavage the Tyr(1605)-Met(1606) scissile bond A2 domain depends on Glu(1660)-Arg(1668) segment same noncatalytic spacer ADAMTS13, suggesting extensive enzyme-substrate...

ADAMTS13 is a metalloproteinase that cleaves von Willebrand factor (VWF) multimers. The metal ion dependence of activity was examined with multimeric VWF and fluorescent peptide substrate based on Asp1596–Arg1668 the A2 domain, FRETS-VWF73. in citrate-anticoagulated plasma enhanced ∼2-fold by zinc ions, ∼3-fold calcium ∼6-fold both suggesting cooperative activation. Cleavage recombinant activated up to ∼200-fold ions (KD app ∼0.5 μm), ∼4.8 barium ∼1.7 mm). Barium stimulated citrated but not...

Regulatory exosite I of thrombin is present on prothrombin in a precursor state (proexosite I) that specifically binds the Tyr63-sulfated peptide, hirudin54–65(Hir54–65(SO3−)) and nonsulfated analog. The role proexosite mechanism factor Va acceleration activation was investigated kinetic studies effects peptide binding. initial rate human by Xa inhibited peptides presence but not absence cofactor. Factor did bind with significant affinity compared prothrombin. Maximum inhibition reduced...

OBJECTIVE The interrelations between obesity, glucose intolerance, hypertension, dyslipidemia, and insulin resistance are well recognized. These relationships of particular interest in Hong Kong's Chinese population, whom increasing affluence has coincided with a marked increase the prevalence NIDDM. We designed pilot study to examine visceral fat cardiovascular risk factors NIDDM patients. RESEARCH DESIGN AND METHODS studied 21 patients whose was quantified by magnetic resonance imaging....

Phosphorylated prolactin has been identified and isolated from bovine pituitaries. The biological activity of this phosphoprotein is severely reduced in comparison with nonphosphorylated prolactin. sites phosphorylation are serines 26, 34, 90, the stoichiometry 1:1:10, respectively. In report, phosphoserine residues have individually replaced glutamic acid recombinant methionyl prolactins order to mimic at each site. Substitution for serine positions 90 protein helical contents by 10, 6,...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantitative comparison of combined gas chromatographic/mass spectrometric profiles complex mixturesDennis H. Smith, Michael. Achenbach, William J. Yeager, Patricia Anderson, L. Fitch, and Thomas C. RindfleischCite this: Anal. Chem. 1977, 49, 11, 1623–1632Publication Date (Print):September 1, 1977Publication History Published online1 May 2002Published inissue 1 September...

Summary The purpose of this cross‐sectional study was to examine the risk associations between obesity indexes body mass index (BMI) and waist circumference (WC)], cardiovascular factors plasma glucose lipids, blood pressure urinary albumin excretion (UAE)] morbidity conditions (Type 2 diabetes mellitus, hypertension, dyslipidaemia and/or albuminuria) in Hong Kong Chinese. Seven‐hundred two Chinese subjects (18–65 years age, 59.4% whom had at least one condition) were recruited from Prince...

The present study was conducted to determine if ethnic differences exist for single oral dose pharmacokinetics of paracetamol and its conjugates between Hong Kong Chinese Caucasian subjects.Twenty healthy (n = 11) 9) subjects, aged 21-44 years, 11 male nine female, were given syrup 20 mg/kg, following an overnight fast. Paracetamol metabolites (glucuronide, sulphate, cysteine mercapturic acid conjugates) measured in serial plasma samples (0.25, 0.5, 0.75, 1.0, 1.5, 2, 3,...,12, 24 h) urine...

The Gram-positive pathogen Streptococcus pyogenes injects a β-NAD+ glycohydrolase (SPN) into the cytosol of an infected host cell using cytolysin-mediated translocation pathway. In this compartment, SPN accelerates death by unknown mechanism that may involve its β-NAD+-dependent enzyme activities. has been reported to possess unique characteristic not only catalyzing hydrolysis β-NAD+, but also carrying out ADP-ribosyl cyclase and ADP-ribosyltransferase activities, making can catalyze all...

Female golden hamsters (Mesocricetus auratus) were housed individually on a photoperiod of LD 6:18 (lights 1000-1600 h). Estrous cyclicity was interrupted for an average 16 wk after which cycles resumed spontaneously. Such animals are photorefractory, remaining cyclic normally nonstimulatory photoperiods. Photorefractory females exposed to continuous darkness in estrous the population rapidly became asynchronous as each hamster's "day" assumed endogenous periodicity (tau) her circadian...

Activation of prothrombin by factor Xa is accompanied expression regulatory exosites I and II on the blood coagulation proteinase, thrombin. Quantitative affinity chromatography equilibrium binding studies with a fluorescein-labeled derivative exosite I-specific peptide ligand, hirudin54–65 ([5F]Hir54–65(SO3−), were employed to identify characterize this site human bovine its [5F]Hir54–65(SO3−) showed distinctive fluorescence excitation spectral differences in complexes thrombin bound...

The functional role of previously identified calcium binding residues in α-lactalbumin (α-LA) was investigated by site-directed mutagenesis. Mutation D82 to alanine did not effect the affinity for calcium, protein structure, or its function lactose synthase assay, suggesting that this aspartate side chain is essential structural stabilization. In contrast, mutation either D87 D88 completely eliminated strong and altered α-LA as shown several spectroscopically derived properties such near-...

ADAMTS proteases typically employ some combination of ancillary C-terminal disintegrin-like, thrombospondin-1, cysteine-rich, and spacer domains to bind substrates facilitate proteolysis by an N-terminal metalloprotease domain. We constructed chimeric examine the role ADAMTS13 ADAMTS5 in recognition their physiological cleavage sites von Willebrand factor (VWF) aggrecan, respectively. cleaves Glu(373)-Ala(374) Glu(1480)-Gly(1481) bonds bovine aggrecan but does not cleave VWF. Conversely,...