A5006329944- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Alkaline Phosphatase Research Studies
- Blood Coagulation and Thrombosis Mechanisms
- Heparin-Induced Thrombocytopenia and Thrombosis
- Venous Thromboembolism Diagnosis and Management
- Air Quality and Health Impacts
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Adenosine and Purinergic Signaling
- Angiogenesis and VEGF in Cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Hemophilia Treatment and Research
- Monoclonal and Polyclonal Antibodies Research
- Climate Change and Health Impacts
- Antimicrobial Resistance in Staphylococcus
- Parathyroid Disorders and Treatments
- Erythrocyte Function and Pathophysiology
- Complement system in diseases
- Pancreatitis Pathology and Treatment
- Diabetes Treatment and Management
- Infective Endocarditis Diagnosis and Management
- Blood disorders and treatments
- Heterotopic Ossification and Related Conditions
KU Leuven
2014-2023
Universitair Ziekenhuis Leuven
2008-2019
Center for Vascular Biology Research
2000-2016
VIB-KU Leuven Center for Cancer Biology
2005-2016
Maastricht University
2003-2016
University of Milan
2016
San Raffaele University of Rome
2016
Leiden University
2005-2014
University Medical Center Utrecht
2014
University of Cambridge
2014
Background — Pollution by particulates has been consistently associated with increased cardiovascular morbidity and mortality. However, the mechanisms responsible for these effects are not well-elucidated. Methods Results To assess to what extent how rapidly inhaled pollutant particles pass into systemic circulation, we measured, in 5 healthy volunteers, distribution of radioactivity after inhalation “Technegas,” an aerosol consisting mainly ultrafine 99m Technetium-labeled carbon (<100...
The kinetics of the activation Glu-plasminogen and Lys-plasminogen (P) by a two-chain form human tissue plasminogen activator (A) were studied in purified systems, presence fibrinogen (f) fibrin films (F) increasing size surface density.
Air pollution has wide-ranging and deleterious effects on human health is a major issue for the global community. The Global Burden of Disease study described worldwide impact air with as many 3.1 million 52.8 all-cause all-age deaths being attributable to ambient in year 2010.1 Moreover, ranked ninth among modifiable disease risk factors, listed above other commonly recognized such low physical activity, high-sodium diet, high cholesterol, drug use. Finally, accounts 3.1%...
The mechanisms of particulate pollution-related cardiovascular morbidity and mortality are not well understood. We studied the passage radioactively labeled ultrafine particles after their intratracheal instillation. Hamsters received a single instillation 100 μ g albumin nanocolloid (nominal diameter ⩽ 80 nm) with Ci technetium-99m were killed 5, 15, 30, 60 min. In blood, radioactivity, expressed as percentage total body radioactivity per gram amounted to 2.88 ± 0.80%, 1.30 0.17%, 1.52...
The fibrinolytic properties of two molecular forms extrinsic (tissue-type) plasminogen activator, purified from human melanoma cells in culture, were compared.One form, obtained under protection aprotinin, consisted a single polypeptide chain with M, = 72,000 while the other without chains 30,000-40,000 each.The had same activity (clot lysis time) system composed fibrin and plasminogen, both dissolved '261-fibrinogenlabeled plasma clots immersed whole at very similar rates.However, sodium...
During chromatography of plasminogen-and fibrinogen-depleted human plasma on an insolubilized fragment plasminogen which contains the high affinity lysine-binding site (LBS I-Sepharose), both az-antiplasmin and another protein are bound subsequently eluted with 10 m 6-aminohexanoic acid.This other was purified to homogeneity by DEAE-Sephadex followed immunoadsorption obtained a yield 2.5 mg/liter plasma.Alternatively, this partially (-80% pure) CM-cellulose LBS I-Sepharose 40 plasma.The...
Quantum dots (QDs) have numerous possible applications for in vivo imaging. However, toxicity data are scarce.To determine the acute of QDs with carboxyl surface coating (carboxyl-QDs) and amine (amine-QDs), we investigated inflammatory properties, tissue distribution, prothrombotic effects after intravenous injection.We performed particle characterization by transmission electron microscopy dynamic light scattering. Carboxyl-QDs amine-QDs were intravenously injected mice (1.44-3,600...
Particulate air pollution is associated with cardiovascular morbidity and mortality. To investigate this association, we studied the effect of ultrafine (60 nm) polystyrene particles on thrombus formation in a hamster model after intravenous intratracheal administration unmodified, carboxylate-polystyrene, or amine-polystyrene particles. Unmodified had no thrombosis up to 5 mg/kg. Carboxylate-polystyrene significantly inhibited at 500 100 μg/kg body weight but not 50 weight. In contrast,...
Pollution by particulates has consistently been associated with increased cardiovascular morbidity and mortality, but a plausible biological basis for this association is lacking.Diesel exhaust particles (DEPs) were instilled into the trachea of hamsters, blood platelet activation, experimental thrombosis, lung inflammation studied. Doses 5 to 500 micro g DEPs per animal induced neutrophil influx bronchoalveolar lavage fluid elevation protein histamine without lactate dehydrogenase release....
Mechanisms regulating thrombus stabilization remain largely unknown. Here, we report that loss of any 1 the Gas6 receptors (Gas6-Rs), i.e., Tyro3, Axl, or Mer, delivery a soluble extracellular domain Axl traps protects mice against life-threatening thrombosis. Loss Gas6-R does not prevent initial platelet aggregation but impairs subsequent aggregates, at least in part by reducing "outside-in" signaling and granule secretion. Gas6, through its receptors, activates PI3K Akt stimulates tyrosine...
Human histidine-rich glycoprotein was found to interact strongly with heparin both in purified systems and plasma, resulting neutralization of the anti-coagulant activity heparin. In systems, react apparent 1:1 stoichiometry form a complex dissociation constant 7 nM. Covalent heparin-antithrombin still reacts 29 The interaction between Mr = 4300-heparin fragment appeared be more complex. mechanism different from that antithrombin III heparin, since former is abolished by EDTA occurs...
We report three novel inhibitors of the physiological pyrophosphatase activity alkaline phosphatase and show that these compounds are capable reducing calcification in two models vascular (i.e., they suppress vitro by cultured Enpp1(-/-) VSMCs inhibit increased a rat aortic model).Genetic ablation tissue-nonspecific (TNALP) leads to accumulation inhibitor inorganic pyrophosphate (PP(i)). TNALP deficiency ameliorates hypermineralization phenotype ank/ank mice, osteoarthritis soft tissue...
ABSTRACT Medial vascular calcification (MVC) is a pathological phenomenon that causes stiffening and can lead to heart failure; it common variety of conditions, including aging, chronic kidney disease, diabetes, obesity, rare genetic diseases. These conditions share the feature tissue-nonspecific alkaline phosphatase (TNAP) upregulation in vasculature. To evaluate role TNAP MVC, we developed mouse model overexpresses human smooth muscle cells an X-linked manner. Hemizygous overexpressor male...
The current authors evaluated whether a system of co-cultures relevant cells (pneumocytes (A549), macrophages (THP-1), mast (HMC-1) and endothelial (EAHY926)) would mimic the responses to particles with 50% cut-off aerodynamic diameter 10 μm (PM ) previously reported in vivo . role was considered special interest. Single cultures, bicultures (A549 + HMC-1 10:1 ratio; THP-1 2:1 ratio) tricultures 10:2:1 were exposed urban PM (24 h at 0, 10, 30 or 100 µg·cm −2 ). Additionally, EAHY926...
During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix by promoting formation hydroxyapatite seed crystals in sheltered interior membrane-limited vesicles (MVs). Here, we have studied phosphosubstrate catalysis osteoblast-derived MVs at physiologic pH, analyzing hydrolysis ATP, ADP, PP(i) isolated wild-type (WT) as well TNAP-, NPP1- PHOSPHO1-deficient MVs. Comparison catalytic efficiencies identified ATP main substrate hydrolyzed...