A5089688879- Angiogenesis and VEGF in Cancer
- Congenital heart defects research
- Mesenchymal stem cell research
- Lymphatic System and Diseases
- Tissue Engineering and Regenerative Medicine
- Protease and Inhibitor Mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Wound Healing and Treatments
- Liver physiology and pathology
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiovascular Disease and Adiposity
- Kruppel-like factors research
- Zebrafish Biomedical Research Applications
- Pluripotent Stem Cells Research
- Cardiac Fibrosis and Remodeling
- Pregnancy and preeclampsia studies
- Cancer, Hypoxia, and Metabolism
- Apelin-related biomedical research
- Birth, Development, and Health
- Cardiac and Coronary Surgery Techniques
- Phagocytosis and Immune Regulation
- Cardiovascular Function and Risk Factors
- Cell Adhesion Molecules Research
- Liver Disease Diagnosis and Treatment
KU Leuven
2015-2024
Center for Vascular Biology Research
2013-2024
VIB-KU Leuven Center for Cancer Biology
2000-2022
Brigham and Women's Hospital
2021
Harvard University
2021
Creative Commons
2017
Universitat de València
2015
Biomedical Research Networking Center on Neurodegenerative Diseases
2015
Universitair Ziekenhuis Leuven
2009
University of Minnesota
2005-2007
VEGF has been shown to be necessary, but not sufficient alone, for the development of subretinal pathologic angiogenesis. In current study, influence placental growth factor (PlGF), a member family, in human and experimental choroidal neovascularization (CNV) was investigated.The presence family mRNA evaluated by RT-PCR neovascular membranes extracted during surgery. The spatial temporal pattern isoforms PlGF expression were explored using laser capture catapulting technique murine...
Epidemiological and histological evidence implicates proteinases of the matrix metalloproteinase (MMP) family in atherosclerosis aneurysm formation. We previously indicated a role for urokinase-type plasminogen activator atherosclerotic media destruction by proteolytic activation MMPs. However, specific MMPs, such as MMP-9 MMP-12, remains undefined.MMP-9- or MMP-12-deficient mice were crossed atherosclerosis-prone apolipoprotein E-deficient background fed cholesterol-rich diet. Mice killed...
Acute kidney injury is followed by regeneration of damaged renal tubular epithelial cells. The purpose this study was to test the hypothesis that stem cells exist in adult and participate repair process. A unique population behave a manner consistent with cell were isolated from rat kidneys termed multipotent progenitor (MRPC). Features these include spindle-shaped morphology; self-renewal for >200 doublings without evidence senescence; normal karyotype DNA analysis; expression vimentin,...
Abstract Several adherent postnatal stem cells have been described with different phenotypic and functional properties. As many of these are being considered for clinical therapies, it is great importance that the identity potency products validated. We compared phenotype characteristics human mesenchymal (hMSCs), mesoangioblasts (hMab), multipotent adult progenitor (hMAPCs) using uniform standardized methods. Human MAPCs could be expanded significantly longer in culture. Differences cell...
One of the key challenges in bone tissue engineering is timely formation blood vessels that promote survival implanted cells construct. Fracture healing largely depends on presence an intact periosteum but it still unknown whether periosteum-derived (PDC) are critical for repair only by promoting or also inducing neovascularization. We first established a protocol to specifically isolate murine PDC (mPDC) from long bones adult mice. Mesenchymal stem were abundantly present this cell...
Endothelial cells (ECs) lining arteries and veins have distinct molecular/functional signatures. The underlying regulatory mechanisms are incompletely understood. Here, we established a specific fingerprint of freshly isolated arterial venous ECs from human umbilical cord comprising 64 12 genes, representing functions/pathways. Among the genes were 8 transcription factors (TFs), including Notch target HEY2, current "gold standard" determinant for EC (aEC) specification. Culture abrogated...
Microvascular endothelium in different organs is specialized to fulfill the particular needs of parenchymal cells. However, specific information about heart capillary endothelial cells (ECs) lacking.Using microarray profiling on freshly isolated ECs from heart, brain, and liver, we revealed a genetic signature for microvascular identified Meox2/Tcf15 heterodimers as novel transcriptional determinants. This was largely shared with skeletal muscle adipose tissue enriched genes encoding fatty...
Background— Monocytes may have an important role in the resolution of venous thrombosis. Increased expression tissue-type plasminogen activator (tPA) and urokinase-type (uPA) is associated with ingress monocytes into thrombus. This study was designed to evaluate importance these activators thrombus resolution. Methods Results— Inferior caval vein thrombosis induced cohorts adult wild-type, uPA gene-knockout (uPA −/− ), tPA (tPA ) mice a flow model. Thrombi were harvested from wild-type (n=60...
The angiogenic mechanism and therapeutic potential of PDGF-CC, a recently discovered member the VEGF/PDGF superfamily, remain incompletely characterized. Here we report that PDGF-CC mobilized endothelial progenitor cells in ischemic conditions; induced differentiation bone marrow into ECs; stimulated migration ECs. Furthermore, smooth muscle their growth during vessel sprouting. Moreover, delivery enhanced postischemic revascularization heart limb. Modulating activity may provide novel...
Background— Placental growth factor (PlGF) has been implicated in the pathophysiological angiogenesis and monocyte recruitment that underlie chronic inflammatory disease, but its role atherosclerosis not examined. We investigated effects of exogenous PlGF, delivered by adenoviral gene transfer, on atherogenic intimal thickening macrophage accumulation induced collar placement around rabbit carotid artery examined PlGF deficiency apolipoprotein E–deficient (apoE −/− ) mice. Methods Results—...
Recently, several populations of postnatal stem cells, such as multipotent adult progenitor cells (MAPCs), have been described that broader differentiation ability than classical cells. Here we compare the transcriptome pluripotent embryonic (ESCs), MAPCs, and lineage-restricted mesenchymal (MSCs) to determine their relationship.Applying principal component analysis, non-negative matrix factorization k-means clustering algorithms gene-expression data, identified a unique profile for MAPCs....
Differentiation of adult bone marrow stem cells (BMSC) into hepatocyte-like is commonly performed by continuous exposure to a cytokines-cocktail. Here, it shown that the differentiation efficacy in vitro can be considerably enhanced sequential addition liver-specific factors (fibroblast growth factor-4, hepatocyte factor, insulin-transferrin-sodium selenite, and dexamethasone) time-dependent order closely resembles secretion pattern during vivo liver embryogenesis. Quantitative RT-PCR...
Despite progress in cardiovascular research, a cure for peripheral vascular disease has not been found. We compared the vascularization and tissue regeneration potential of murine human undifferentiated multipotent adult progenitor cells (mMAPC-U hMAPC-U), MAPC-derived progenitors (mMAPC-VP), unselected BM (mBMCs) mice with moderate limb ischemia, reminiscent intermittent claudication patients. mMAPC-U durably restored blood flow muscle function stimulated regeneration, by direct trophic...