A5078508470- Bone fractures and treatments
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Connective tissue disorders research
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- Biomedical Ethics and Regulation
- Wnt/β-catenin signaling in development and cancer
- Electrospun Nanofibers in Biomedical Applications
- Connective Tissue Growth Factor Research
- TGF-β signaling in diseases
- Bone health and treatments
- Bone and Dental Protein Studies
- Cannabis and Cannabinoid Research
- Orthopaedic implants and arthroplasty
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Bone health and osteoporosis research
- MicroRNA in disease regulation
- Periodontal Regeneration and Treatments
- Biological and pharmacological studies of plants
- Spondyloarthritis Studies and Treatments
Royal Veterinary College
2009-2025
University College London
2014-2022
UCB Pharma (United Kingdom)
2018-2022
Stanmore College
2020-2022
KU Leuven
2009-2021
Box (United States)
2016
Royal National Orthopaedic Hospital
2014
University of Nottingham
2008-2011
Rosetta Stone (United States)
2002
Abstract Several adherent postnatal stem cells have been described with different phenotypic and functional properties. As many of these are being considered for clinical therapies, it is great importance that the identity potency products validated. We compared phenotype characteristics human mesenchymal (hMSCs), mesoangioblasts (hMab), multipotent adult progenitor (hMAPCs) using uniform standardized methods. Human MAPCs could be expanded significantly longer in culture. Differences cell...
One of the key challenges in bone tissue engineering is timely formation blood vessels that promote survival implanted cells construct. Fracture healing largely depends on presence an intact periosteum but it still unknown whether periosteum-derived (PDC) are critical for repair only by promoting or also inducing neovascularization. We first established a protocol to specifically isolate murine PDC (mPDC) from long bones adult mice. Mesenchymal stem were abundantly present this cell...
Abstract Background Bones have a remarkable capacity to heal upon fracture. Yet, in large defects or compromised conditions healing processes become impaired, resulting delayed non-union. Current therapeutic approaches often utilize autologous allogeneic bone grafts for augmentation. However, limited availability of these tissues and lack predictive biological response result limitations clinical demands. Tissue engineering using viable cell-based implants is strategic approach address unmet...
In this study, we investigated a clinically relevant model of in vivo ectopic bone formation utilizing human periosteum derived cells (HPDCs) seeded Collagraft carrier and explored the mechanisms by which process is driven. Bone occurred after eight weeks when minimum one million HPDCs was loaded on carriers implanted subcutaneously NMRI nu/nu mice. De novo matrix, mainly secreted HPDCs, found juxta-proximal calcium phosphate (CaP) granules suggesting that CaP may have triggered...
Objectives Interleukin (IL)-17 signalling has been shown to be a key regulator of disease in ankylosing spondylitis (AS) with several IL-17 blockers currently clinically approved. Despite this, the role bone pathology is poorly understood. This study aimed investigate context pathological formation. Methods A biomimetic human periosteum-derived cell (hPDC) model osteogenic differentiation was used combination recombinant cytokines, T-cell supernatants or serum from patients AS. IL-17A,...
Advances in genetic research have provided a basis for susceptibility testing Alzheimer disease (AD). Prior surveys examined attitudes toward AD hypothetical scenarios, but it is unclear what reasons would motivate people to seek real-life situations. This study presents data from the first randomized trial evaluate asymptomatic adult children of with AD. We (1) endorsed as motivations seeking testing, (2) demographic characteristics associated these reasons, and (3) how related eventual...
DNA measurement and RNA extraction are two frequently used methods for cell characterization. In the conventional protocols, they require similar, but separate samples in most cases, different pretreatments. The few combined protocols that exist still include time-consuming steps. Hence, to establish an efficient protocol two-dimensional (2D) three-dimensional (3D) cultures, a PicoGreen-based was integrated existing protocol. It validated by analysis of influence lysis buffers, RLT, RA1, or...
The identity of the cells that form periosteum during development is controversial with current dogma suggesting these are derived from a Sox9-positive progenitor. Herein, we characterize newly created Prrx1eGFP reporter transgenic mouse line limb formation and postnatally. Interestingly, in embryo Prrx1eGFP-labeled become restricted around cartilage anlage without themselves becoming Sox9-positive. In adult, transgene live labels subpopulation within enriched at specific sites, this...
OPINION article Front. Vet. Sci., 07 March 2022Sec. Veterinary Regenerative Medicine https://doi.org/10.3389/fvets.2022.817041
Abstract Sclerosteosis, an ultra-rare disorder characterised by high bone mass (HBM) and skeletal overgrowth, leads to facial paralysis, hearing loss raised intracranial pressure, which is currently managed only through high-risk surgery. Sclerosteosis caused SOST mutations of functional sclerostin, a protein that suppresses osteogenesis antagonising Wnt/β-catenin signalling. Herein, using in vitro vivo approaches, we explore whether LGK974, another potent Wnt inhibitor targets porcupine...
Bone tissue engineering is currently undergoing a paradigm shift regarding the concepts used to develop cell-based therapies for skeletal repair. In place of "trial and error" approach, researchers aim at developing cellular that mirror developmental postnatal processes. Herein, we describe model in vivo endochondral remodeling an vitro derived cartilaginous intermediate its applicability bone engineering. differentiation continuous cell line, ATDC5, pellet culture was enhanced medium...
Osteosarcoma management continues to lack the appropriate prognostic tools assign personalised treatment. This leaves non-responders standard care vulnerable recurring disease and pulmonary metastases. Developing 3D in vitro models serve as a test bed for treatment is promising approach address this issue. study describes generation of osteosarcoma termed "tumouroids", which are geometrically compartmentalised reproduce bone cancer mass its surrounding. Although tumour microenvironment...
During endochondral ossification, chondrocyte growth and differentiation is controlled by many local signalling pathways. Due to crosstalks feedback mechanisms, these interwoven pathways display a network like structure. In this study, large-scale literature based logical model of the plate was developed. The able capture different states (resting, proliferating hypertrophic) that chondrocytes go through as they progress within plate. first corroboration step, effect mutations in various...
Abstract We report the ability to move and arrange patterns of live embryonic stem cells using holographic optical tweezers. Single cell suspensions mouse were manipulated with tweezers into a variety including lines, curves circles. Individual also lifted out sample plane highlighting potential for 3D positional control. Trypan blue dye exclusion Live/Dead™ staining (CMFDA−1, EthHD−1) showed that still viable after manipulation The individual specific, pre-defined provides method study how...
Human mesenchymal stromal cells (hMSCs) offer great potential for bone tissue engineering applications, but their in vivo performance remains limited. Preconditioning of these with small molecules to improve differentiation before implantation, or incorporation growth factors are possible solutions. Insulin-like factor-1 (IGF-1) is one the most abundant bone, involved growth, development, and metabolism, its effects on hMSCs still subject debate. Here we examined IGF-1 proliferation vitro...
Strategies for bone regeneration are undergoing a paradigm shift, moving away from the replication of end-stage tissue and instead aiming to recapture initial events fracture repair. Although this is known resemble endochondral formation, chondrogenic cell types with favorable proliferative hypertrophic differentiation properties lacking. Recent advances in cellular reprogramming have allowed creation alternative populations specific through forced expression transcription factors. Herein,...