A5087526715- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Structural Anomalies and Repair
- Birth, Development, and Health
- Hormonal Regulation and Hypertension
- Congenital heart defects research
- Mesenchymal stem cell research
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- Electrolyte and hormonal disorders
- Elasticity and Material Modeling
- Renal and related cancers
- Renin-Angiotensin System Studies
- Renal and Vascular Pathologies
- Bone Tissue Engineering Materials
- Renal function and acid-base balance
- CRISPR and Genetic Engineering
- Muscle and Compartmental Disorders
- Aldose Reductase and Taurine
- Redox biology and oxidative stress
- Cellular Mechanics and Interactions
- Digestive system and related health
- Pharmaceutical studies and practices
- Biomedical Research and Pathophysiology
- Additive Manufacturing and 3D Printing Technologies
University of Minnesota
2009-2021
Minneapolis Heart Institute Foundation
2016-2021
Hospital General Universitario Gregorio Marañón
2012
Institute of Cardiology
2006
Fairview Health Services
1997
Twin Cities Orthopedics
1986-1996
Vanderbilt University
1991
The renin-angiotensin-aldosterone system (RAAS) participates in the injury sustained by remnant kidney. Our studies assessed importance of aldosterone that model and response to drugs interfering with RAAS. Initially, four groups rats were studied: SHAM-operated rats, untreated (REM), REM treated losartan enalapril (REM AIIA), AIIA infused exogenous + ALDO). last group was maintained levels comparable those constant infusion aldosterone. had larger adrenal glands a > 10-fold elevation plasma...
Acute kidney injury is followed by regeneration of damaged renal tubular epithelial cells. The purpose this study was to test the hypothesis that stem cells exist in adult and participate repair process. A unique population behave a manner consistent with cell were isolated from rat kidneys termed multipotent progenitor (MRPC). Features these include spindle-shaped morphology; self-renewal for >200 doublings without evidence senescence; normal karyotype DNA analysis; expression vimentin,...
Perfusion decellularization of cadaveric hearts removes cells and generates a cell-free extracellular matrix scaffold containing acellular vascular conduits, which are theoretically sufficient to perfuse support tissue-engineered heart constructs. However, after transplantation, these conduits clot, even with anti-coagulation. Here, our objective was create less thrombogenic improve recellularized-left ventricular contractility by re-lining decellularized rat aortic endothelial (RAECs).We...
Dietary protein restriction imposed before renal injury is established in the remnant kidney model rat reduces glomerular hypertension and hyperperfusion injury. We demonstrate that dietary (6% vs. 20%) on a background of leads to greater preservation function as measured by filtration rate fractional clearances albumin IgG, despite persistence systemic hypertension. In similarly prepared rats, led lower capillary hydraulic pressure, higher ultrafiltration coefficient, similar single nephron...
ABSTRACT. X-linked Alport syndrome (XLAS) is a progressive disorder of basement membranes caused by mutations in the <i>COL4A5</i> gene, encoding α5 chain type IV collagen. A mouse model this was generated targeting human nonsense mutation, G5X, to <i>Col4a5</i> gene. As predicted for hemizygous mutant male mice are null and heterozygous carrier female mosaic α5(IV) expression. Mutant viable through reproductive age fertile. died spontaneously at 6 34 wk age, 8 45 manifesting proteinuria,...
Tissue-engineered heart valves (TEHV) have been proposed as a promising solution for the clinical needs of pediatric patients. In vivo studies shown TEHV leaflet contraction and regurgitation after several months implantation. This has attributed to contractile cells utilized produce extracellular matrix (ECM) during culture. Here, we such develop mature ECM in fibrin-based scaffold that generates commissural alignment leaflets then removed these using detergents. Further, evaluated...
Aldosterone participates in the pathophysiology of several models progressive chronic renal disease. Because causal connection between transforming growth factor-beta(1) (TGF-beta) and scarring many such models, we hypothesized that aldosterone could evoke TGF-beta kidney. infusion for 3 days otherwise normal rats caused a more than twofold increase excretion without changes systolic pressure or evidence kidney damage. Concurrent treatment with amiloride did not alter this effect, indicating...
Background. Female carriers of X-linked Alport syndrome (XLAS) demonstrate variability in clinical phenotype that, unlike males, cannot be correlated with genotype. X-inactivation, the method by which females (XX) silence transcription from one X chromosome order to achieve gene dosage parity males (XY), likely modifies carrier phenotype, but this hypothesis has not been tested directly.
To expand the application of perfusion decellularization beyond isolated single organs, we used native vasculature adult and neonatal rats to systemically decellularize organs a whole animal in situ. Acellular scaffolds were generated from kidney, liver, lower limb, heart-lung system, body, demonstrating that technology is applicable any perfusable tissue, independent age. Biochemical histological analyses demonstrated organ systems (heart-lung pair limb) successfully decellularized,...
The discoidin domain receptors (DDRs) DDR1 and DDR2 are cardinal members of a receptor tyrosine kinase subfamily, activated by collagens. They candidate effectors in tissue injury fibrosis. We investigated the DDR expression normal remnant rat kidneys.The kidney other tissues was examined indirect immunofluorescence, immunoblotting, ribonuclease protection assays. patterns control kidneys were compared at 2-, 4-, 8-week time points, following induction injury.DDR1 is expressed basolateral...
Perfusion decellularization of cadaveric hearts removes cells and generates a cell-free extracellular matrix scaffold containing acellular vascular conduits, which are theoretically sufficient to perfuse support tissue-engineered heart constructs. This article contains additional data our experience decellularizing testing structural integrity composition large series human hearts, "Acellular matrix: critical step toward whole heat grafts" (Sanchez et al., 2015) [1]. Here we provide the...
Diffuse leiomyomatosis is associated with the inherited kidney disease Alport syndrome, and characterized by visceral smooth muscle overgrowth within respiratory, gastrointestinal female reproductive tracts. Although partial deletions of type IV collagen genes COL4A5 COL4A6, paired head-to-head on chromosome Xq22, are known to cause diffuse leiomyomatosis, loss function for does not explain overgrowth. To further clarify pathogenic mechanisms, we have novel in patients syndrome-diffuse or...
The mutant Mpv17 mouse is a transgenic strain that fails to express protein normally expressed in the kidney and associated with peroxisomes. present studies provide quantitative examination of renal function structure this compared its control CFW strain. By 52 wk age, developed proteinuria (urinary creatinine ratio: 25 +/- 14 versus 3 1, control), albuminuria albumin 23 15 0.1 0.1, hypoalbuminemia (2.1 0.4 2.5 0.2 G/dl, but without arterial hypertension or major reduction filtration (serum...