A5034141505- Heme Oxygenase-1 and Carbon Monoxide
- Neonatal Health and Biochemistry
- Clinical practice guidelines implementation
- Hemoglobinopathies and Related Disorders
- Renal Diseases and Glomerulopathies
- Hemoglobin structure and function
- Central Venous Catheters and Hemodialysis
- Chronic Kidney Disease and Diabetes
- Dialysis and Renal Disease Management
- Renal and Vascular Pathologies
- COVID-19 and healthcare impacts
- Acute Kidney Injury Research
- Cardiac Arrest and Resuscitation
- Pregnancy and preeclampsia studies
- Renal function and acid-base balance
- Iron Metabolism and Disorders
- Birth, Development, and Health
- COVID-19 Clinical Research Studies
- Vascular Procedures and Complications
- Eicosanoids and Hypertension Pharmacology
- Thermal Regulation in Medicine
- Cardiovascular Health and Risk Factors
- Muscle and Compartmental Disorders
- Electrolyte and hormonal disorders
- Patient Satisfaction in Healthcare
Mayo Clinic
2016-2025
Conway School of Landscape Design
2025
Mayo Clinic in Arizona
2000-2024
Health Affairs
2018-2023
Mayo Clinic in Florida
2001-2023
Cedars-Sinai Medical Center
2023
Advisory Board Company (United States)
2023
WinnMed
2004-2021
Hypertension Institute
2010-2021
University of Bergamo
2021
Phagocyte-mediated oxidant damage to vascular endothelium is likely involved in various vasculopathies including atherosclerosis and pulmonary leak syndromes such as adult respiratory distress syndrome.We have shown that heme, a hydrophobic iron chelate, rapidly incorporated into endothelial cells where, after little 1 h, it markedly aggravates cytotoxicity engendered by polymorphonuclear leukocyte oxidants or hydrogen peroxide (H20z).In contrast, however, if cultured are briefly pulsed with...
Heme proteins such as myoglobin or hemoglobin, when released into the extracellular space, can instigate tissue toxicity. Myoglobin is directly implicated in pathogenesis of renal failure rhabdomyolysis. In glycerol model this syndrome, we demonstrate that kidney responds to inordinate amounts heme by inducing heme-degradative enzyme, oxygenase, well increasing synthesis ferritin, major cellular repository for iron. Prior recruitment response with a single preinfusion hemoglobin prevents and...
Iron-derived reactive oxygen species are implicated in the pathogenesis of various vascular disorders including atherosclerosis, vasculitis, and reperfusion injury. The present studies examine whether heme, when liganded to physiologically relevant proteins as hemoglobin, can provide potentially damaging iron intact endothelium. We demonstrate that reduced ferrohemoglobin, while relatively innocuous cultured endothelial cells, oxidized ferrihemoglobin (methemoglobin), greatly amplifies...
The human end-stage kidney and its experimental analogue, the remnant in rat, exhibit widespread tubulo-interstitial disease. We investigated whether pathogenesis of such injury is dependent upon adaptive changes tubular function and, particular, ammonia production when renal mass reduced. Dietary acid load was reduced 1 3/4-nephrectomized rats by dietary supplementation with sodium bicarbonate (NaHCO3), while control rats, paired for serum creatinine after 3/4 nephrectomy, were supplemented...
Renal pathologists and nephrologists met on February 20, 2015 to establish an etiology/pathogenesis-based system for classification diagnosis of GN, with a major aim standardizing the kidney biopsy report GN. On basis etiology/pathogenesis, GN is classified into following five pathogenic types, each specific disease entities: immune-complex pauci-immune antiglomerular basement membrane monoclonal Ig C3 glomerulopathy. The pathogenesis-based forms report. To standardize report, consists...
IgA nephropathy frequently leads to progressive CKD. Although interest surrounds use of immunosuppressive agents added standard therapy, several recent studies have questioned efficacy these agents. Depleting antibody-producing B cells potentially offers a new therapy. In this open label, multicenter study conducted over 1-year follow-up, we randomized 34 adult patients with biopsy-proven and proteinuria >1 g/d, maintained on angiotensin-converting enzyme inhibitors or angiotensin receptor...
Membranous nephropathy (MN) is a pattern of injury caused by autoantibodies binding to specific target antigens, with accumulation immune complexes along the subepithelial region glomerular basement membranes. The past 20 years have brought revolutionary advances in understanding MN, particularly via discovery novel antigens and their respective autoantibodies. These discoveries challenged traditional classification MN into primary secondary forms. At least 14 been identified, accounting for...
The pathophysiological mechanisms responsible for maintenance of chronic renovascular hypertension remain undefined. Excess angiotensin II generation may lead to release reactive oxygen species and increased vasoconstrictor activity. To examine the potential involvement oxidation-sensitive in pathophysiology hypertension, blood samples were collected renal flow measured with electron-beam computed tomography pigs 5 10 weeks after induction unilateral artery stenosis (n=7) or sham operation...
Hydrogen peroxide (H2O2) contributes to renal cellular injury. alpha-Keto acids nonenzymatically reduce H2O2 water while undergoing decarboxylation at the 1-carbon (1-C) position. We examined, in vitro and vivo, protective role of sodium pyruvate H2O2-induced Pyruvate effectively scavenged vitro, suppressed lipid peroxidation. Injury LLC-PK1 cells induced by hydrogen was attenuated an extent comparable that seen with catalase. Studies utilizing [1-14C]pyruvate further demonstrated 1-C...
The arteriovenous fistula (AVF) is the preferred hemodialysis access, but AVF-failure rate high, and complications from AVF placement are rarely reported. There no clear consensus on predictors of patency. This study determined outcomes patency at Mayo Clinic Rochester following Fistula First Initiative.A retrospective cohort AVFs placed January 2006 through December 2008 was performed. placement-associated primary secondary failure rates, complications, interventions, hospitalizations were...