A5031890969- Plant Pathogens and Fungal Diseases
- Insect Resistance and Genetics
- Nematode management and characterization studies
- Real-Time Systems Scheduling
- Plant-Microbe Interactions and Immunity
- Soybean genetics and cultivation
- Entomopathogenic Microorganisms in Pest Control
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Research in Cotton Cultivation
- Insect-Plant Interactions and Control
- Distributed and Parallel Computing Systems
- Embedded Systems Design Techniques
- Mycotoxins in Agriculture and Food
- Plant pathogens and resistance mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Liver Disease Diagnosis and Treatment
- Transgenic Plants and Applications
- Parallel Computing and Optimization Techniques
- Insect symbiosis and bacterial influences
- Plant biochemistry and biosynthesis
- Acute Kidney Injury Research
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Cassava research and cyanide
Iowa State University
2012-2024
SRM Institute of Science and Technology
2024
Bombay Natural History Society
2022
Apollo Hospitals
2015-2018
Pusan National University
2014
International Centre for Genetic Engineering and Biotechnology
2003-2009
International Centre for Genetic Engineering and Biotechnology
2002-2007
Mayo Clinic
2007
Indian Agricultural Research Institute
2004
Dependable Computing (United States)
2002
Insecticidal crystal proteins of Bacillus thuringiensis bind to receptors in the midgut susceptible insects leading pore formation and death insect. The identity receptor is not clearly established. Recently a direct interaction between cloned heterologously expressed aminopeptidase (slapn) from Spodoptera lituraand Cry1C protein was demonstrated by immunofluorescence andin vitro ligand blot interaction. Here we show that administration slapn double-stranded RNA S. litura larvae reduces its...
Abstract Biosynthetic gene clusters are common in microbial organisms, but rare plants, raising questions regarding the evolutionary forces that drive their assembly multicellular eukaryotes. Here, we characterize biochemical function of a rice (Oryza sativa) cytochrome P450 monooxygenase, CYP76M7, which seems to act production antifungal phytocassanes and defines second diterpenoid biosynthetic cluster rice. This is uniquely multifunctional, containing enzymatic genes involved two distinct...
Recent reports have revealed genomic clustering of enzymatic genes for particular biosynthetic pathways in plant specialized/secondary metabolism. Rice (Oryza sativa) carries two such clusters production antimicrobial diterpenoid phytoalexins, with the cluster on chromosome 2 containing four closely related/homologous members cytochrome P450 CYP76M subfamily (CYP76M5-8). Notably, underlying evolutionary expansion these CYP appears to occurred after assembly ancestral gene cluster, suggesting...
Aminopeptidase-N (APN) and cadherin proteins located at the midgut epithelium of <i>Helicoverpa armigera</i> have been implicated as receptors for Cry1A subfamily insecticidal <i>Bacillus thuringiensis</i>. Ligand blot analysis with heterologously expressed purified <i>H. Bt receptor three closely related tentatively identified HaAPN1 an interacting ligand. However, to date there is no direct evidence APN being a functional Cry1Ac in armigera</i>. Sf21 insect cells expressing displayed...
The bacterium Bacillus thuringiensis produces ICPs (insecticidal crystal proteins) that are deposited in their spore mother cells. When susceptible lepidopteran larvae ingest these cells, the get solubilized alkaline gut environment. Of approx. 140 insecticidal proteins described thus far, protein Cry1Ac has been applied extensively as main ingredient of spray formulation well principal ICP introduced into crops transgene for agricultural crop protection. 135 kDa protein, upon ingestion by...
Fusarium virguliforme causes sudden death syndrome (SDS) in soybean. The pathogen has never been isolated from diseased foliar tissues; therefore, one or more toxins have considered to cause SDS development. Cell-free F. culture filtrates containing a toxin A low-molecular-weight protein of approximately 13.5 kDa (FvTox1), purified filtrates, produces SDS-like symptoms cut soybean seedlings. Anti-FvTox1 monoclonal antibodies raised against the FvTox1 were used isolating gene. In presence...
The cotton textile industry is based on the material properties of terminally differentiated trichoblasts. Tens thousands epidermal trichoblasts emerge from seed coat and march through a reproducible developmental program that includes interconversion between cell-autonomous tissue-based morphogenesis. Proteomic analyses purified fibers provide way to associate molecules with cellular processes directly affect morphological transitions harvested cells. However, insufficient temporal sampling...
Several investigators have independently identified membrane-associated aminopeptidases in the midgut of insect larvae as initial interacting ligand to insecticidal crystal proteins Bacillus thuringiensis. Though several isoenzymes been from an and their corresponding cDNA cloned, only one isoform has expressed heterologously studied for its binding Cry toxins. Here we report cloning expression two N Helicoverpa armigera (American cotton bollworm) (HaAPNs). The full-length H. APN1 (haapn1)...
ABSTRACT The economic value of cotton is based on its long, thin, strong, and twisted trichoblasts that emerge from the ovule epidermis. mature dried fiber cell reflects outcome a rapid tapering nascent trichoblast, weeks polarized diffuse growth, followed by transition to persistent secondary wall synthesis. Highly conserved dynamic microtubule cellulose microfibril-based anisotropic growth control modules are central all these phases. In this paper, we developed novel quantitative...
Cotton fiber is the most valuable naturally available material for textile industry and length strength are key determinants of its quality. Dynamic changes in pectin, xyloglucan, xylan, cellulose polysaccharide epitope content during growth contribute to complex remodeling cell wall (CW) Detailed knowledge about compositional structural alteration elongation strengthening important understand molecular dynamics development improve Here, large-scale glycome profiling coupled with phenotype...
Fusarium virguliforme causes the serious disease sudden death syndrome (SDS) in soybean. Host resistance to this pathogen is partial and encoded by a large number of quantitative trait loci, each conditioning small effects. Breeding SDS therefore challenging identification single-gene novel mechanisms becoming priority fight devastating fungal pathogen. In transcriptomic study we identified few putative soybean defense genes, expression which suppressed during F. infection. The...
Arabidopsis (Arabidopsis thaliana) root development is regulated by multiple dynamic growth cues that require central metabolism pathways such as β-oxidation and auxin. Loss of the pectin biosynthesizing enzyme GALACTURONOSYLTRANSFERASE 10 (GAUT10) leads to a short-root phenotype under sucrose-limited conditions. The present study focused on determining specific contributions GAUT10 composition in primary roots underlying defects associated with gaut10 roots. Using live-cell microscopy, we...
Abstract Cotton fiber length and strength are key determinants of its quality. Dynamic changes in the pectin, xyloglucan, xylan, cellulose polysaccharide epitopes content during growth contribute to complex remodeling cell wall (CW) Detailed knowledge about compositional structural alteration elongation strengthening is vastly limited. Here, large-scale glycome profiling coupled with phenotype transcriptome was conducted on collected daily covering most critical developmental window. High...
Nonhost resistance is defined as the immunity of a plant species to all nonadapted pathogen species. Arabidopsis (Arabidopsis thaliana) ecotype Columbia-0 nonhost oomycete Phytophthora sojae and fungal Fusarium virguliforme that are pathogenic soybean (Glycine max). Previously, we reported generating pss1 mutation in pen1-1 genetic background well mapping characterization sojae-susceptible gene locus, PSS1. In this study, identified six candidate PSS1 genes by comparing single-nucleotide...
Fusarium virguliforme is a soil borne root pathogen that causes sudden death syndrome (SDS) in soybean [Glycine max (L.) Merrill]. Once the fungus invades xylem tissues, secretes toxins cause chlorosis and necrosis foliar tissues leading to defoliation, flower pod drop eventually of plants. Resistance F. partial governed by over 80 quantitative trait loci (QTL). We have conducted genome-wide association study (GWAS) for group 254 plant introductions lines using panel approximately 30,000...