A5013889724- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Renal Transplantation Outcomes and Treatments
- Complement system in diseases
- Systemic Lupus Erythematosus Research
- Renal and Vascular Pathologies
- Renal and related cancers
- Dialysis and Renal Disease Management
- Nephrotoxicity and Medicinal Plants
- Parathyroid Disorders and Treatments
- Electrolyte and hormonal disorders
- Renin-Angiotensin System Studies
- Acute Kidney Injury Research
- Autoimmune Bullous Skin Diseases
- Cell Adhesion Molecules Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Chronic Lymphocytic Leukemia Research
- Transplantation: Methods and Outcomes
- Renal cell carcinoma treatment
- Biomedical Research and Pathophysiology
- Muscle and Compartmental Disorders
- Blood disorders and treatments
- T-cell and B-cell Immunology
- Ion Transport and Channel Regulation
Harvard University
2016-2025
Brigham and Women's Hospital
2016-2025
Harvard University Press
1983-2022
RELX Group (United States)
2017
Dana-Farber Cancer Institute
2003-2016
Beth Israel Deaconess Medical Center
2016
World Economic Forum
2016
Palo Alto Institute
2016
Dialyse Centrum Groningen
2012-2016
University of Arizona
2016
Micropuncture studies were performed in three groups of male Munich-Wistar rats 1 wk after surgery: group I, eight control that underwent laparotomy and fed a normal diet; II, nine right nephrectomy segmental infarction five-sixths the left kidney III, seven same renal ablative procedure low protein diet. Single nephron glomerular filtration rate (SNGFR) was higher remnant II compared with I due to average values for mean transcapillary hydraulic pressure difference (delta P) initial plasma...
Two groups of adult male Munich-Wistar rats and a third group nondiabetic age-matched weight-matched normal control underwent micropuncture study 1 mo, morphologic studies 14 after induction streptozotocin diabetes or sham treatment. All animals were fed standard rat chow. Diabetic received daily ultralente insulin to maintain stable moderate hyperglycemia (approximately 350 mg/dl). In addition, one diabetic was treated with the angiotensin I converting enzyme inhibitor, enalapril, 15...
Micropuncture and morphologic studies were performed in six groups of male Munich-Wistar rats after removal the right kidney segmental infarction two-thirds left kidney. Groups 1 4 received no specific therapy. 2 5 treated with angiotensin I-converting enzyme inhibitor, enalapril, 50 mg/liter, drinking water. 3 6 reserpine (5 mg/liter), hydralazine (80 hydrochlorothiazide (25 mg/liter). All fed standard chow. 1-3 underwent micropuncture study wk renal ablation. Untreated group exhibited...
Micropuncture and morphologic studies were performed in four groups of male Munich-Wistar rats after removal the right kidney segmental infarction two-thirds left kidney. Groups 1 3 received no specific therapy. 2 4 treated with angiotensin I converting enzyme inhibitor, enalapril, 50 mg/liter which was put their drinking water. All fed standard chow. underwent micropuncture study wk renal ablation. Untreated group exhibited systemic hypertension elevation single nephron glomerular...
ABSTRACT A mutation was targeted to the murine α3 integrin gene. Homozygous mutant mice survived birth, but died during neonatal period. The caused abnormal kidney and lung development. Mutant kidneys displayed decreased branching of medullary collecting ducts, although number nephrons not altered. Proximal tubules exhibited two distinct subsets abnormalities, with epithelial cells either containing excess lysosomes or becoming microcystic. In addition, glomerular development markedly...
Six groups of Munich-Wistar rats underwent micropuncture study 2-10 weeks and morphologic studies 11-13 months after induction streptozotocin diabetes or sham treatment. Diabetic received diets containing 6% (group D6), 12% (D12), 50% protein (D50) were maintained under similar conditions moderate hyperglycemia by daily injections ultralente insulin. Age- weight-matched normal control also given (Group N6), (N12), (N50). Kidney weight, whole-kidney single-nephron glomerular filtration rate,...
To determine the role of aldosterone in mediating cardiovascular damage, we performed ablation/replacement experiments with a rat model cardiac injury. Administration angiotensin II and Nω-nitro-l-arginine methyl ester (L-NAME; nitric oxide synthesis inhibitor) to male rats drinking 1% saline caused hypertension, severe biventricular myocardial necrosis, proteinuria, fibrinoid necrosis renal vessels. Removal by adrenalectomy or through administration selective antagonist eplerenone markedly...
Diabetic nephropathy is characterized by damage to both the glomerulus and tubulointerstitium, but relatively little known about accompanying cell-specific changes in gene expression. We performed unbiased single-nucleus RNA sequencing (snRNA-seq) on cryopreserved human diabetic kidney samples generate 23,980 transcriptomes from 3 control early samples. All major cell types of were represented final dataset. Side-by-side comparison demonstrated cell-type-specific expression that are...
Mouse kidneys were perfused with Krebs-Ringer bicarbonate buffer (KRB) containing native, anionic horse spleen ferritin or various cationized derivatives, and the glomerular localization of probe molecules determined by electron microscopy. Ferritins cationic respect to medium (KRB, pH 7.45) accumulated in subendothelial layers basement membrane (GBM) amounts far exceeding those observed ferritins, degree being greater for more derivatives. Strongly addition permeated full thickness GBM...
Renal pathologists and nephrologists met on February 20, 2015 to establish an etiology/pathogenesis-based system for classification diagnosis of GN, with a major aim standardizing the kidney biopsy report GN. On basis etiology/pathogenesis, GN is classified into following five pathogenic types, each specific disease entities: immune-complex pauci-immune antiglomerular basement membrane monoclonal Ig C3 glomerulopathy. The pathogenesis-based forms report. To standardize report, consists...
Background Few studies have evaluated whether histopathologic lesions on kidney biopsy provide prognostic information beyond clinical and laboratory data.Methods We enrolled 676 individuals undergoing native at three tertiary care hospitals into a prospective, observational cohort study. Biopsy specimens were adjudicated for semiquantitative scores in 13 categories of histopathology by two experienced renal pathologists. Proportional hazards models tested the association between risk disease...
Since its emergence in Wuhan, China, coronavirus disease 2019 (COVID-19) has spread rapidly throughout the world. Severe acute respiratory syndrome 2 (SARS-CoV-2) primarily targets lung, but other organs may be affected, and kidney involvement is frequent. Recent data from New York City found a 36.6% incidence of AKI among 5499 hospitalized patients with COVID-19.1 Of those who developed AKI, 14.3% required RRT this study. Among mechanical ventilation, 86.9% 23.2% intubated RRT. The...
Significance Statement Although corticosteroids are an effective first-line therapy for minimal change disease, relapse, steroid dependence, and intolerance common in this podocytopathy of unknown etiology. The efficacy B cell–targeted therapies some patients suggests autoantibody-mediated This study describes the novel discovery both adults children with disease autoantibodies targeting nephrin, a critical component podocyte slit diaphragm that ensures integrity glomerular filtration...
Membranous nephropathy (MN) is a pattern of injury caused by autoantibodies binding to specific target antigens, with accumulation immune complexes along the subepithelial region glomerular basement membranes. The past 20 years have brought revolutionary advances in understanding MN, particularly via discovery novel antigens and their respective autoantibodies. These discoveries challenged traditional classification MN into primary secondary forms. At least 14 been identified, accounting for...
The immunosuppressive effect of rapamycin is mediated by inhibition interleukin-2-stimulated T cell proliferation. We report for the first time that also inhibits growth factor-induced proliferation cultured mouse proximal tubular (MPT; IC 50 ∼1 ng/ml) cells and promotes apoptosis these impairing survival effects same factors. On basis in vitro data, we tested hypothesis would impair recovery renal function after ischemic acute failure induced vivo artery occlusion (RAO). Rats given daily...
Excerpt In 1953, Billingham described a state of "actively acquired tolerance" to skin allografts developing after transfer viable allogeneic cells fetal or neonatal mice (1). This was followed by the identification Mitchison "radiation chimaera" (2) and demonstration Main Prehn increased survival specific donor strain in x-irradiated bone marrow reconstituted hosts (3). More recently, Strober (4) reported cases three patients treated with total lymphoid x-radiation who subsequently accepted...