A5004456379- Renal Diseases and Glomerulopathies
- Autoimmune Bullous Skin Diseases
- Vasculitis and related conditions
- Chronic Kidney Disease and Diabetes
- Complement system in diseases
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Platelet Disorders and Treatments
- Dialysis and Renal Disease Management
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Genetic and Kidney Cyst Diseases
- Renal Transplantation Outcomes and Treatments
- Electrolyte and hormonal disorders
- Systemic Sclerosis and Related Diseases
- Systemic Lupus Erythematosus Research
- Biomedical Research and Pathophysiology
- Potassium and Related Disorders
- Parathyroid Disorders and Treatments
- Pharmacological Effects and Toxicity Studies
- Renal and related cancers
- Renal function and acid-base balance
- Renal and Vascular Pathologies
- Tuberous Sclerosis Complex Research
- Pregnancy and Medication Impact
- Muscle and Compartmental Disorders
- Immunodeficiency and Autoimmune Disorders
Boston Medical Center
2016-2025
Boston University
2016-2025
University of Michigan
2022
University Medical Center
2011-2021
RELX Group (United States)
2018-2021
International Society of Nephrology
2020
University of Utah
2016
National Kidney Foundation
2016
Augusta University
2016
American Society of Nephrology
2016
Idiopathic membranous nephropathy, a common form of the nephrotic syndrome, is an antibody-mediated autoimmune glomerular disease. Serologic diagnosis has been elusive because target antigen unknown.We performed Western blotting protein extracts from normal human glomeruli with serum samples patients idiopathic or secondary nephropathy other proteinuric diseases and controls. We used mass spectrometry to analyze reactive bands confirmed identity location monospecific antibody.Serum 26 37...
Idiopathic membranous nephropathy is an autoimmune disease. In approximately 70% of patients, it associated with autoantibodies against the phospholipase A2 receptor 1 (PLA2R1). Antigenic targets in remaining patients are unknown.
The vascular system forms through a combination of vasculogenesis and angiogenesis. In vasculogenesis, vessels form de novo via the assembly endothelial precursors called angioblasts, whereas in angiogenesis new arise by migration proliferation cells from preexisting vessels. Although two processes are distinct some respects, recent evidence suggests that they share number regulatory mechanisms. identification defined growth factors, observations genetically manipulated mice, recognition...
Autoantibodies to the M-type phospholipase A(2) receptor (PLA(2)R) are sensitive and specific for idiopathic membranous nephropathy. The anti-B cell agent rituximab is a promising therapy this disease, but biomarkers of early response treatment currently do not exist. Here, we investigated whether levels anti-PLA(2)R correlate with immunological activity nephropathy, potentially exhibiting more rapid than clinical parameters such as proteinuria. We measured amount using Western blot...
The M-type phospholipase A2 receptor (PLA2R) is a target autoantigen in adult idiopathic membranous nephropathy (MN), but the prevalence of autoantibodies against PLA2R unknown among Chinese patients with MN. Here, we measured anti-PLA2R antibody serum 60 MN, 20 lupus-associated 16 hepatitis B (HBV)-associated and 10 tumor-associated Among 49 (82%) had detectable using Western blot assay; an assay greater sensitivity detected very low titers remaining 11 patients. Using standard assay, only...
Summary Background and objectives Circulating autoantibodies against the M-type phospholipase A 2 receptor (anti-PLA R) were recently identified in majority of patients United States with idiopathic membranous nephropathy (iMN). The this study to assess prevalence anti-PLA R a separate, European cohort iMN correlate presence clinical parameters reflective disease activity. Design, setting, participants, & measurements Anti-PLA levels blindly assessed by Western blot immunoassay 54 serum...
Thrombospondin type 1 domain–containing 7A (THSD7A) is a target antigen identified in adult membranous nephropathy (MN) along with the major phospholipase A 2 receptor (PLA R1). The prevalence of THSD7A-Ab–positive patients unknown, and it unclear whether clinical presentation differs between positive for PLA R1-Ab or THSD7A-Ab. We screened serum samples 1276 MN from three different cohorts presence THSD7A-Ab by Western blot analysis newly developed indirect immunofluorescence test (IFT)....
Significance Statement Although corticosteroids are an effective first-line therapy for minimal change disease, relapse, steroid dependence, and intolerance common in this podocytopathy of unknown etiology. The efficacy B cell–targeted therapies some patients suggests autoantibody-mediated This study describes the novel discovery both adults children with disease autoantibodies targeting nephrin, a critical component podocyte slit diaphragm that ensures integrity glomerular filtration...
Membranous nephropathy (MN) is a pattern of injury caused by autoantibodies binding to specific target antigens, with accumulation immune complexes along the subepithelial region glomerular basement membranes. The past 20 years have brought revolutionary advances in understanding MN, particularly via discovery novel antigens and their respective autoantibodies. These discoveries challenged traditional classification MN into primary secondary forms. At least 14 been identified, accounting for...
Uncontrolled complement activation can cause or contribute to glomerular injury in multiple kidney diseases. While plays a causal role atypical hemolytic uremic syndrome (aHUS) and C3 glomerulopathy (C3G), over the past decade rapidly accumulating body of evidence has shown for other diseases, including diabetic nephropathy several glomerulonephritides. The number available inhibitor therapies also increased during same period. In 2022 KDIGO convened Controversies Conference, Role Complement...
There is a need to define regions of gene activation or repression that control human kidney cells in states health, injury, and repair understand the molecular pathogenesis disease design therapeutic strategies. Comprehensive integration expression with epigenetic features regulatory elements remains significant challenge. We measure dual single nucleus RNA chromatin accessibility, DNA methylation, H3K27ac, H3K4me1, H3K4me3, H3K27me3 histone modifications decipher landscape regulation...
Abstract —Embryological data suggest that endothelial cells (ECs) direct the recruitment and differentiation of mural cell precursors. We have developed in vitro coculture systems to model some these events shown ECs migration undifferentiated mesenchymal (10T1/2 cells) induce their toward a smooth muscle cell/pericyte lineage. The present study was undertaken investigate proliferation cocultures. 10T1/2 were cocultured an underagarose assay absence contact. There 2-fold increase...
WHILE evaluating patients with the syndrome of inappropriate secretion antidiuretic hormone, it became apparent to me that hypouricemia was common during period hyponatremia. Review literature showed only one report associated syndrome,1 and in recent reviews hospital patients, either not mentioned2 , 3 or found.4 Therefore, a retrospective study serum levels uric acid hyponatremic performed. The results indicate is rule hormone but also concentrations urate can .
Primary membranous nephropathy (pMN) is a leading cause of nephrotic syndrome in adults. In most cases, this autoimmune kidney disease associated with autoantibodies against the M-type phospholipase A2 receptor (PLA2R1) expressed on podocytes, but mechanisms to glomerular damage remain elusive. Here, we developed cell culture model using human podocytes and found that anti-PLA2R1–positive pMN patient sera or isolated IgG4, not IgG4-depleted sera, induced proteolysis 2 essential podocyte...
H.P. Acthar® Gel is currently the only Food and Drug Administration therapy approved for treatment of nephrotic syndrome. Active drug ingredients include structurally related melanocortin peptides that bind to cell surface G-protein-coupled receptors known as receptors, which are expressed in glomerular podocytes. In animal models membranous nephropathy, stimulation has been demonstrated reduce podocyte injury loss. We hypothesized would improve symptoms syndrome patients with idiopathic...
Thrombospondin type I domain-containing 7A (THSD7A) is a known antigenic target of autoantibodies leading to primary membranous glomerulopathy and was reported account for ~10% phospholipase A2 receptor (PLA2R)-negative glomerulopathy. It has been proposed that PLA2R THSD7A are mutually exclusive in We validated an immunohistochemical assay investigate THSD7A-associated utilized it 258 consecutive native kidney biopsies, which showed our laboratory, with the exception lupus nephritis....