A5073925292- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Collagen: Extraction and Characterization
- Cardiac Valve Diseases and Treatments
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Tendon Structure and Treatment
- Elasticity and Material Modeling
- Wound Healing and Treatments
- Cell Adhesion Molecules Research
- Connective tissue disorders research
- Mathematical Biology Tumor Growth
- Cardiac and Coronary Surgery Techniques
- Blood properties and coagulation
- Hemostasis and retained surgical items
- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Aortic Disease and Treatment Approaches
- Congenital Heart Disease Studies
- Nerve injury and regeneration
- Lipid Membrane Structure and Behavior
- Gene Regulatory Network Analysis
- Immune Response and Inflammation
University of Minnesota
2016-2025
Twin Cities Orthopedics
2015
University of Minnesota System
2001-2014
University of Florida
2012
American Medical Research
2009
Bose (United States)
2006
Boston University
2006
Minneapolis Institute of Arts
1997
University of Washington Applied Physics Laboratory
1993
Applied Mathematics (United States)
1993
We present a general mathematical theory for the mechanical interplay in tissue-equivalents (cell-populated collagen gels): Cell traction leads to compaction of fibrillar network, which certain conditions such as constraint or inhomogeneous cell distribution, can result and consequently fibril alignment, leading contact guidance, affects subsequent compaction. The accounts intrinsically biphasic nature gel, is comprised network interstitial solution. also alignment due deformation, that is,...
ABSTRACT Despite the likely role of contact guidance in every physiological process involving cell migration, its study a three-dimensional tissue-equivalent environment has been precluded, heretofore, by inherent difficulties systematically preparing well-defined fields and quantifying resultant guidance. Here, we describe novel use magnetic field to orient collagen fibrils during fibrillogenesis, entrapping cells dispersed solution. Using computer-controlled staging image analysis, show...
Abstract We report here on studies examining the use of fibrin as an alternative to collagen for entrapment neonatal aortic rat smooth muscle cells (SMCs) in fabrication media equivalents. The show increased production by fibroblasts entrapped fibrin, which suggests that may be used tissue equivalents promote protein synthesis and remodeling. However, one challenges working with is rapid degradation SMCs. This was effectively inhibited addition ϵ‐aminocaproic acid (EACA) culture medium...
Glycation, the nonenzymatic crosslinking of proteins by reducing sugars, is known to cause stiffening soft tissues over a lifetime, particularly in diabetics. We show here that glycation due elevated glucose and ribose concentrations cell culture medium can be exploited matter few weeks incubation stiffen strengthen tissue equivalents increase their resistance collagenolytic degradation, all without loss viability. Glycated did not elicit inflammation or induce calcification upon...
Abstract We report here, with respect to collagen production and the mechanical properties of a fibrin‐based media equivalent (ME), on our efforts optimize culture conditions neonatal SMCs entrapped in tubular fibrin gels. examined several factors, including concentration fibrinolysis inhibitor, cell source initial number, addition TGF‐β insulin medium, time culture. found that varying ϵ‐aminocaproic acid (ACA), an inhibitor fibrinolysis, did not affect production, but lower concentrations...
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation David M. Knapp, Victor H. Barocas, Alice G. Moon, Kyeongah Yoo, Linda R. Petzold, Robert T. Tranquillo; Rheology of reconstituted type I collagen gel in confined compression. Journal 1 September 1997; 41 (5): 971–993. https://doi.org/10.1122/1.550817 Download citation file: Ris (Zotero) Reference Manager...
Smooth muscle formation and function are critical in development postnatal life. Hence, studies aimed at better understanding SMC differentiation of great importance. Here, we report that multipotent adult progenitor cells (MAPCs) isolated from rat, murine, porcine, human bone marrow demonstrate the potential to differentiate into with an SMC-like phenotype function. TGF-β1 alone or combined PDGF-BB serum-free medium induces a temporally correct expression transcripts proteins consistent...
Tissue engineering provides a means to create functional living tissue replacements. Here, we examine the effects of 3 weeks cyclic distension (CD) on fibrin-based tubular constructs seeded with porcine valve interstitial cells. CD circumferential strain amplitude ranging from 2.5% 20% was applied evaluate fibrin remodeling into tissue. We hypothesized that during long-term cells adapt constant (constant CD), diminishing growth. thus also subjected incremented 5% 15% over [incremental...
A high-potential therapy for repairing the heart post-myocardial infarction is implantation of tissue-engineered myocardium. While several groups have developed constructs that mimic aligned structure native myocardium, to date no one has investigated particular functional benefits conferred by alignment. In this study we created myocardial in both and isotropic configurations entrapping neonatal rat cardiac cells fibrin gel. Constructs were cultured statically 2 weeks, then characterized....
Treatment of congenital heart defects in children requiring right ventricular outflow tract reconstruction typically involves multiple open-heart surgeries because all existing graft materials have no growth potential. Here we present an 'off-the-shelf' vascular grown from donor fibroblasts a fibrin gel to address this critical unmet need. In proof-of-concept study, the decellularized grafts are implanted as pulmonary artery replacement three young lambs and evaluated adulthood. Longitudinal...
The mechanical environment plays an important role in cell signaling and tissue homeostasis. Unraveling connections between externally applied loads the cellular response is often confounded by extracellular matrix (ECM) heterogeneity. Image-based multiscale models provide a foundation for examining fine details of behavior, but they require validation at multiple scales. In this study, we developed model that captured anisotropy heterogeneity cell-compacted collagen gel subjected to...
The performance of completely biological, decellularized engineered allografts in a sheep model was evaluated to establish clinical potential these unique arterial allografts. 4-mm-diameter, 2-3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube vitro by ovine dermal fibroblasts. Decellularization and subsequent storage had little effect on graft properties, with burst pressure exceeding 4000 mmHg the same compliance as femoral artery. Grafts implanted...
Prosthetic arteriovenous grafts (AVGs) conventionally used for hemodialysis are associated with inferior primary patency rates and increased risk of infection compared autogenous vein grafts. We tissue-engineered an AVG grown from neonatal human dermal fibroblasts entrapped in bovine fibrin gel that is then decellularized. This graft both "off-the-shelf" (nonliving) completely biological. Grafts 6 mm diameter about 15 cm length were evaluated a baboon model access axillary-cephalic or...