A5114377333- Pancreatic function and diabetes
- Veterinary Oncology Research
- Mesenchymal stem cell research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Diabetes and associated disorders
- Acute Myeloid Leukemia Research
- Diabetes Treatment and Management
- Veterinary Medicine and Surgery
- Cancer-related molecular mechanisms research
- Neuropeptides and Animal Physiology
- RNA modifications and cancer
- Cancer Research and Treatments
- Alzheimer's disease research and treatments
- Immunotherapy and Immune Responses
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Glycosylation and Glycoproteins Research
- Diabetes Management and Research
- Pluripotent Stem Cells Research
- Poetry Analysis and Criticism
- Molecular Biology Techniques and Applications
- Sarcoma Diagnosis and Treatment
- Diet and metabolism studies
- Phagocytosis and Immune Regulation
- Cancer Genomics and Diagnostics
Peter MacCallum Cancer Centre
2025
St Vincent's Hospital
2025
University of Minnesota
2013-2024
Louis Stokes Cleveland VA Medical Center
2023-2024
Guy's and St Thomas' NHS Foundation Trust
2024
University of Minnesota Medical Center
2010-2021
University Hospitals Seidman Cancer Center
2021
Cleveland Clinic
2021
University Hospitals of Cleveland
2020
University of Minnesota System
2018
Amyloid deposits localized to the islets of Langerhans are typical non-insulin-dependent human diabetes mellitus and in adult cats. also commonly occur insulin-producing pancreatic tumors. We have purified a major protein--insulinoma or islet amyloid polypeptide (IAPP)--from cat from insulinoma. IAPP insulinoma contained 37 amino acid residues had theoretical molecular mass 3850 Da. The sequence is unique but has greater than 40% identity with calcitonin gene-related peptide. A partial...
Prosthetic arteriovenous grafts (AVGs) conventionally used for hemodialysis are associated with inferior primary patency rates and increased risk of infection compared autogenous vein grafts. We tissue-engineered an AVG grown from neonatal human dermal fibroblasts entrapped in bovine fibrin gel that is then decellularized. This graft both "off-the-shelf" (nonliving) completely biological. Grafts 6 mm diameter about 15 cm length were evaluated a baboon model access axillary-cephalic or...
Abstract Tissue organoids are a promising technology that may accelerate development of the societal and NIH mandate for precision medicine. Here we describe robust simple method generating cerebral (cOrgs) from human pluripotent stem cells by using chemically defined hydrogel material culture medium. By no additional neural induction components, cOrgs appeared on surface within 10–14 days, under static conditions, they attained sizes up to 3 mm in greatest dimension day 28. Histologically,...
Abstract Overall survival of patients with osteosarcoma (OS) has improved little in the past three decades, and better models for study are needed. OS is common large dog breeds genetically inducible mice, making disease ideal comparative genomic analyses across species. Understanding level conservation intertumor transcriptional variation species how it associated progression to metastasis will enable us more efficiently develop effective strategies manage improve therapy. In this study,...
Inhibition of dipeptidyl peptidase IV (DPP-IV) activity by NVP-DPP728, a DPP-IV inhibitor, improves the therapeutic efficacy glucagon-like peptide-1 (GLP-1). CD26 is membrane-associated glycoprotein with and expressed on lymphocytes. We investigated effect NVP-DPP728 reversing new-onset diabetes in nonobese diabetic (NOD) mice modulating inflammatory response stimulating β-cell regeneration. New-onset NOD were treated for 2, 4, 6 wk. Blood glucose level was monitored. Regulatory T cells...
We performed genomewide gene expression analysis of 35 samples representing 6 common histologic subtypes canine lymphoma and bioinformatics analyses to define their molecular characteristics. Three major groups were defined on the basis profiles: (1) low-grade T-cell lymphoma, composed entirely by T-zone lymphoma; (2) high-grade consisting lymphoblastic peripheral not otherwise specified; (3) B-cell marginal diffuse large Burkitt lymphoma. Interspecies comparative profiles also showed that...
Canine hemangiosarcomas have been ascribed to an endothelial origin based on histologic appearance; however, recent findings suggest that these tumors may arise instead from hematopoietic progenitor cells. To clarify this ontogenetic dilemma, we used genome-wide expression profiling of primary and identified three distinct tumor subtypes associated with angiogenesis (group 1), inflammation 2), adipogenesis 3). Based findings, hypothesized a common differentiate into the observed in our gene...
In Brief Background Extracellular matrix (ECM) scaffolds, obtained through detergent-based decellularization of native kidneys, represent the most promising platform for investigations aiming at manufacturing kidneys transplant purposes. We previously showed that human kidney yields renal ECM scaffolds (hrECMs) maintain their basic molecular components, are cytocompatible, stimulate angiogenesis, and show an intact innate vasculature. However, evidence preserves glomerular morphometric...
Stem cell-derived hepatocyte-like cells hold great potential for the treatment of liver disease and drug toxicity screening. The success these applications hinges on generation differentiated with high specific activities. Many protocols have been developed to guide human embryonic stem (hESCs) differentiate hepatic lineage. Here we report cultivation hESCs as three-dimensional aggregates that enhances their differentiation cells. Differentiation was first carried out in monolayer culture 20...
A high proportion of patients with cystic fibrosis (CF) develop diabetes mellitus. In common type II mellitus, mellitus in CF is characterized by a progressive decline beta-cell function and an approximately 50% mass. It not known whether islet amyloidosis (characteristic mellitus) present complicating CF. To address this, pancreatic samples were obtained at autopsy from 9 control cases (without CF) 41 that were, turn, subdivided into 13 nondiabetic, 12 borderline diabetic, 16 diabetic based...
We show that multipotent adult progenitor cells (MAPCs) can be derived from both postnatal and fetal swine bone marrow (BM). Although MAPC (swMAPC) cultures are initially mixed, phenotypically homogenous by 50 population doublings (PDs) maintained as such for more than 100 PDs. swMAPCs negative CD44, CD45, major histocompatibility complex (MHC) classes I II; express octamer binding transcription factor 3a (Oct3a) mRNA protein at levels close to those seen in human ESCs (hESCs); have...
Rats from four experimental treatment groups, including fed controls, 24- to 30-h fasted, dexamethasone-treated, and intraperitoneal glucose-treated, were used assess the effects of these treatments on immunohistochemically detectable islet amyloid polypeptide (IAPP) content in pancreatic islets. Isolated perfused pancreases additional animals groups insulin IAPP secretion relative amounts hormones secreted into perfusate under low-glucose (2.75 mM) high-glucose (16.7 conditions. Insulin...
Amyloid deposits that characteristically form in the pancreatic islets of patients with non-insulin-dependent diabetes mellitus (NIDDM) and insulinomas are both derived from islet amyloid polypeptide (IAPP). Evidence previous studies has suggested deposition IAPP-derived is related to inherent amyloidogenic sequences present within normal human IAPP, together an increased production local concentration IAPP. However, whether aggregation IAPP fibrils primarily intra- or extracellular event...