A5086698091- Bone Metabolism and Diseases
- Receptor Mechanisms and Signaling
- Diabetes Treatment and Management
- Pancreatic function and diabetes
- Neuropeptides and Animal Physiology
- Osteoarthritis Treatment and Mechanisms
- NF-κB Signaling Pathways
- Bone health and treatments
- Natural product bioactivities and synthesis
- Estrogen and related hormone effects
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Bioactive Compounds and Antitumor Agents
- Ubiquitin and proteasome pathways
- Angiogenesis and VEGF in Cancer
- Cytokine Signaling Pathways and Interactions
- Peroxisome Proliferator-Activated Receptors
- Hypothalamic control of reproductive hormones
- Cancer, Hypoxia, and Metabolism
- Bioactive natural compounds
- Inflammatory mediators and NSAID effects
- Synthesis and bioactivity of alkaloids
- Adipose Tissue and Metabolism
Shanghai Sunshine Rehabilitation Center
2021-2025
Guizhou Provincial People's Hospital
2024-2025
China Medical University
2024
East China Normal University
2013-2022
Shanghai Changzheng Hospital
2012-2021
MicroPort Orthopedics (Netherlands)
2020
Fudan University Shanghai Cancer Center
2016-2020
Shanghai Medical College of Fudan University
2020
The First People's Hospital of Guiyang
2020
Amgen (United States)
2006-2018
Articular cartilage has limited self-regenerative capacity and the therapeutic methods for defects are still dissatisfactory in clinic. Recent studies showed that exosomes derived from mesenchymal stem cells promoted chondrogenesis by delivering bioactive substances to recipient cells, indicating might be a novel method repairing defect. Herein, we investigated role mechanism of human umbilical cord small extracellular vesicles (hUC-MSCs-sEVs) on regeneration. In vitro results hUC-MSCs-sEVs...
Abstract Prostaglandin E2 (PGE2), a major cyclooxygenase-2 (COX-2) product, is highly secreted by the osteoblast lineage in subchondral bone tissue of osteoarthritis (OA) patients. However, NSAIDs, including COX-2 inhibitors, have severe side effects during OA treatment. Therefore, identification novel drug targets PGE2 signaling progression urgently needed. Osteoclasts play critical role homeostasis and OA-related pain. mechanisms which regulates osteoclast function subsequently are largely...
Long-chain fatty acids amplify insulin secretion from the pancreatic beta-cell. The G-protein-coupled receptor GPR40 is specifically expressed in beta-cells and activated by acids; however, its role acute regulation of vivo remains unclear. To this aim, we generated knockout (KO) mice examined glucose homeostasis, response to Intralipid vivo, vitro after short- long-term exposure acids. Our results show that KO have essentially normal tolerance glucose. Insulin was reduced approximately 50%....
Abstract Gambogic acid (GA), the main active compound of Gamboge hanburyi, has been previously reported to activate apoptosis in many types cancer cell lines by targeting transferrin receptor and modulating nuclear factor-κB signaling pathway. Whether GA inhibits angiogenesis, which is crucial for other human diseases, remains unknown. Here, we found that significantly inhibited umbilical vascular endothelial (HUVEC) proliferation, migration, invasion, tube formation, microvessel growth at...
G-protein-coupled receptor (GPCR) 48 (Gpr48; Lgr4), a newly discovered member of the glycoprotein hormone subfamily GPCRs, is an orphan GPCR unknown function. Using knockout mouse model, we have characterized essential roles Gpr48 in bone formation and remodeling. Deletion mice results dramatic delay osteoblast differentiation mineralization, but not chondrocyte proliferation maturation, during embryonic formation. Postnatal remodeling also significantly affected Gpr48(-/-) mice, including...
Cucurbitacin E (CuE, α-elaterin), a tetracyclic triterpenes compound from folk traditional Chinese medicine plants, has been shown to inhibit cancer cell growth, inflammatory response and bilirubin–albumin binding. However, the effects of CuE on tumor angiogenesis its potential molecular mechanism are still unknown. Here, we demonstrated that significantly inhibited human umbilical vascular endothelial (HUVEC) proliferation, migration tubulogenesis in vitro blocked chick embryo...
TRAF [TNF (tumour necrosis factor)-receptor-associated factor] 2 and 6 are essential adaptor proteins for the NF-κB (nuclear factor κB) signalling pathway, which play important roles in inflammation immune response. Polyubiquitination of TRAF2 TRAF6 is critical to their activities functions TNFα- IL (interleukin)-1β-induced activation. However, regulation by deubiquitination remains incompletely understood. In present study, we identified USP (ubiquitin-specific protease) 4 as a novel...
Type 2 diabetes is characterized by impaired glucose homeostasis due to defects in insulin secretion, resistance and the incretin response. GPR40 (FFAR1 or FFA1) a G-protein-coupled receptor (GPCR), primarily expressed insulin-producing pancreatic β-cells incretin-producing enteroendocrine cells of small intestine. Several agonists, including AMG 837 TAK-875, have been disclosed, but no synthetic agonists reported that engage both insulinogenic incretinogenic axes. In this report we provide...
Tumor necrosis factor alpha (TNFalpha) has been used to treat certain tumors in clinic trials. However, the curative effect of TNFalpha undermined by induced-NF-kappaB activation many types tumor. Maslinic acid (MA), a pharmacological safe natural product, known for its important effects as anti-oxidant, anti-inflammatory, and anti-viral activities. The aim this study was determine whether MA potentiates anti-tumor activity though regulation NF-kappaB activation.In study, we demonstrate that...
Activation of FFA1 (GPR40), a member G protein-coupling receptor family A, is mediated by medium- and long-chain fatty acids leads to amplification glucose-stimulated insulin secretion, suggesting potential role for free acid 1 (FFA1) as target type 2 diabetes. It was assumed previously that there single binding site synthetic agonists. However, using members two chemical series partial full agonists have been identified, radioligand interaction studies revealed the do not bind same but...
Article7 December 2020Open Access Source Data Reprogramming immunosuppressive myeloid cells facilitates immunotherapy for colorectal cancer Weiqiang Lu Corresponding Author [email protected] orcid.org/0000-0003-1349-6597 Shanghai Key Laboratory of Regulatory Biology, Institute Biomedical Sciences and School Life Sciences, East China Normal University, Shanghai, ChinaThese authors contributed equally to this work. Search more papers by author Weiwei Yu Jiacheng He Wenjuan Liu Junjie Yang...
Using an ethnobotanical approach in combination with vivo-guided fractionation as a means for lead discovery, cryptolepine was isolated antihyperglycemic component of Cryptolepis sanguinolenta. Two syntheses cryptolepine, including unambiguous synthesis, are reported. The hydroiodide, hydrochloride, and hydrotrifluoromethanesulfonate (hydrotriflate) salts were synthesized, comparison their spectral properties vitro activities 3T3-L1 glucose transport assay is made. Cryptolepine its salt...
The development of the anterior segment mammalian eye is critical for normal ocular function, whereas abnormal can cause glaucoma, a leading blindness in world. We report that orphan G protein-coupled receptor 48 (Gpr48/LGR4) plays an important role structure. Disruption Gpr48 causes wide spectrum dysgenesis (ASD), including microphthalmia, iris hypoplasia, irdiocorneal angle malformation, cornea dysgenesis, and cataract. Detailed analyses reveal defective myogenesis extracellular matrix...
Abstract Activation of NF-κB and MAPK/activator protein 1 (AP-1) signaling pathways by receptor activator ligand (RANKL) is essential for osteoclast activity. Targeting MAPK/AP-1 to modulate activity has been a promising strategy osteoclast-related diseases. In this study we examined the effects maslinic acid (MA), pentacyclic triterpene that widely present in dietary plants, on RANKL-induced osteoclastogenesis, function, vitro vivo assay systems. mouse bone marrow monocytes (BMMs) RAW264.7...
Inhibition of dipeptidyl peptidase IV (DPP-IV) activity by NVP-DPP728, a DPP-IV inhibitor, improves the therapeutic efficacy glucagon-like peptide-1 (GLP-1). CD26 is membrane-associated glycoprotein with and expressed on lymphocytes. We investigated effect NVP-DPP728 reversing new-onset diabetes in nonobese diabetic (NOD) mice modulating inflammatory response stimulating β-cell regeneration. New-onset NOD were treated for 2, 4, 6 wk. Blood glucose level was monitored. Regulatory T cells...
Kisspeptin-10 (Kp-10), a decapeptide derived from the primary translation product of KISS1 gene, has been reported previously to be key hormone for puberty and an inhibitor tumor metastasis via activation G protein-coupled receptor 54. However, whether Kp-10 inhibits angiogenesis, which is critical growth other human diseases, still unknown. Here we show that significantly umbilical vein endothelial cell (HUVEC) migration, invasion, tube formation, processes in angiogenesis. Using chicken...