A5101782018- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Cancer-related gene regulation
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Genetic factors in colorectal cancer
- Kruppel-like factors research
- Colorectal Cancer Treatments and Studies
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Genomics, phytochemicals, and oxidative stress
- Mesenchymal stem cell research
- Ferroptosis and cancer prognosis
- Neuroendocrine Tumor Research Advances
- Metastasis and carcinoma case studies
- Immune Cell Function and Interaction
- FOXO transcription factor regulation
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
Chinese University of Hong Kong
2016-2025
Hebei Medical University
2013-2025
Third Hospital of Hebei Medical University
2025
First Hospital of Jiaxing
2024
Dali University
2024
Chinese University of Hong Kong, Shenzhen
2012-2022
Prince of Wales Hospital
2011-2020
First Affiliated Hospital of Hebei Medical University
2013
East China Normal University
2010
Abstract Gut microbial dysbiosis contributes to the development of colorectal cancer (CRC). Here we catalogue communities in human gut mucosae at different stages tumorigenesis. We analyse mucosal microbiome 47 paired samples adenoma and adenoma-adjacent mucosae, 52 carcinoma carcinoma-adjacent 61 healthy controls. Probabilistic partitioning relative abundance profiles reveals that a metacommunity predominated by members oral is primarily associated with CRC. Analysis shows differences...
The role of cancer cell FOXP3 in tumorigenesis is conflicting. We aimed to study expression and regulation, function clinical implication human non-small lung (NSCLC).One hundred six patients with histologically-confirmed NSCLC who underwent surgery were recruited for the study. Tumor samples lines used examine its related molecules. Various functions performed. In vivo mouse tumor xenograft was confirm vitro results.NSCLC high level had a significant decrease overall survival...
Cucurbitacin E (CuE, α-elaterin), a tetracyclic triterpenes compound from folk traditional Chinese medicine plants, has been shown to inhibit cancer cell growth, inflammatory response and bilirubin–albumin binding. However, the effects of CuE on tumor angiogenesis its potential molecular mechanism are still unknown. Here, we demonstrated that significantly inhibited human umbilical vascular endothelial (HUVEC) proliferation, migration tubulogenesis in vitro blocked chick embryo...
Deregulated miRNAs participate in colorectal carcinogenesis. In this study, miR-218 was found to be downregulated human cancer (CRC) by miRNA profile assay. silenced or all five colon cells (Caco2, HT29, SW620, HCT116 and LoVo) relative normal tissues. expression significantly lower 46 CRC tumor tissues compared with their adjacent (P < 0.001). Potential target genes of were predicted BMI1 polycomb ring finger oncogene (BMI-1), a oncogene, identified as one the potential targets....
Abstract miR-375 is a tumor-suppressive microRNA (miRNA) in gastric cancer (GC). However, its molecular mechanism remains unclear. The aim of this study to comprehensively investigate how involved Hippo pathway by targeting multiple oncogenes. expression cell lines and primary GC was investigated qRT-PCR. regulation YAP1, TEAD4, CTGF evaluated qRT-PCR, western blot, luciferase reporter assays, respectively. functional roles the related genes were examined siRNA-mediated knockdown or ectopic...
miR-139-5p was identified to be significantly down-regulated in colon tumor tissues by miRNA array. We aimed clarify its biological function, molecular mechanisms and direct target gene colorectal cancer (CRC). The function of examined cell growth, cycle apoptosis analysis vitro vivo. signaling pathway luciferase activity assay western blot. primary (P < 0.0001). Ectopic expression lines suppressed growth as evidenced viability 0.001) colony formation 0.01) xenograft nude mice 0.01). induced...
Gastric cancer (GC) is one of the frequent causes cancer-related death in eastern Asian population. IGF2BP2 lists top rank up-regulated genes GC, but its functional role unclear.The expression IGF2BP3 GC cell lines and primary samples was examined by qRT-PCR Western blot. The biological revealed a series vitro studies. Its regulation microRNAs (miRNAs) predicted TargetScan confirmed luciferase assays rescue experiments.IGF2BP3 ranked No.1 microarray analysis lines. level observed tissues...
Detecting microRNA (miRNA) in stool is a novel approach for colorectal cancer (CRC) screening. This study aimed to identify stool-based miRNA as noninvasive biomarkers detection of CRC and adenoma.A expression array covering 667 human miRNAs was performed on five pairs two advanced adenoma tissues. The most upregulated were validated 40 tissues, 16 424 samples, including 104 CRCs, 169 adenomas, 42 inflammatory bowel diseases (IBD), 109 healthy controls. levels followed-up after removal...
MicroRNAs (miRNAs) have been reported to play an important role in tumorigenesis. In this study, the of miR-15a and miR-16-1 gastric adenocarcinoma (GAC) was investigated. The expression cell lines primary tumors examined by miRNA qRT-PCR. Proliferative assays, colony formation, invasion migration, flow cytometry analysis vivo study were performed ectopic miR-16-1. putative target genes explored TargetScan further validated. We found that down-regulated GAC tumor samples compared with normal...
The detection of microRNA (miRNA) dysregulation in stool is a novel approach for the diagnosis colorectal carcinoma (CRC). aim this study to investigate use miR-221 and miR-18a samples as non-invasive biomarkers CRC diagnosis. A miRNA expression array containing 667 miRNAs was performed identify tissues. We focused on miR-18a, two significantly upregulated which were subsequently verified 40 pairs tissues 595 (198 CRCs, 199 polyps 198 normal controls). array. levels also higher tumours...
Dysregulated microRNA (miRNA) expression was profiled through a miRNA array comparison between human colorectal cancer tumors and their adjacent normal tissues. Specifically, using laser capture micro-dissection, miR-133a shown to be significantly downregulated in primary specimens compared with matched tissue. Ectopic of suppressed cell growth vitro vivo. Cell-cycle analysis revealed that induced G0/G1-phase arrest, concomitant the upregulation key G1-phase regulator p21(Cip1). We further...
Background miR-18a is one of the most up-regulated miRNAs in colorectal cancers (CRC) based on miRNA profiling. In this study, we examined functional significance CRC. Methods Expression was investigated 45 CRC patients. Potential target genes were predicted by silico search and confirmed luciferase activity assay Western blot. DNA damage measured comet assay. Gene function cell viability, colony formation apoptosis assays. Results The up-regulation validated primary tumors compared with...
TEA domain (TEAD) transcription factors are key components of the Hippo-YAP1 signaling pathway, but their functional role and regulatory mechanisms remain unclear. This study aims to comprehensively explore expression pattern TEAD family in gastric carcinogenesis investigate its regulation by microRNAs (miRNAs). The mRNA protein were examined quantitative reverse transcription-PCR (qRT-PCR) western blot. Their roles determined vitro vivo studies. clinicopathological association TEAD4 cancer...
Gene amplification is a hallmark of cancer and frequently observed in colorectal cancer. Previous whole-genome sequencing clinical specimens identified Ring finger protein 6 (RNF6), RING-domain E3 ubiquitin ligase. In this study, we showed that RNF6 upregulated 73.5% (147/200) patients with was positively associated gene amplification. Furthermore, expression its were independent prognostic factors for poor outcome promoted cell growth, cell-cycle progression, epithelial-to-mesenchymal...
NF-κB signaling pathway plays an important role in gastric carcinogenesis. The basic expression and functional of NFKB1 RELA (components canonical pathway) cancer (GC) have not been well elucidated. In this study, the tumorigenesis will be investigated their regulation by microRNAs (miRNAs) deeply explored. mRNA protein were qRT-PCR Western blot GC cell lines primary tumors. roles demonstrated MTT proliferation assay, monolayer colony formation, invasion migration, cycle analysis vivo study...
DNA methylation has been identified as a hallmark of gastric cancer (GC). Identifying genes that are repressed by promoter is essential in providing insights into the molecular pathogenesis cancer. Using genome-wide studies, we transcription factor forkhead box F2 (FOXF2) was preferentially methylated We then investigated functional significance and clinical implication FOXF2 silenced cell lines tissues methylation, which negatively associated with mRNA expression. Ectopic expression...
Yin Yang 1 (YY1) is a transcription factor that regulates diverse biological processes and increasing recognized to have important roles in carcinogenesis. The function clinical significance of YY1 gastric adenocarcinoma (GAC) not been elucidated. In this study, the functional role cancer was investigated by MTT proliferation assays, monolayer colony formation, cell cycle analysis, signaling pathway Western blot analysis vivo study through knockdown or overexpression. Immunohistochemical...
Abstract Background Cancer‐associated fibroblasts (CAFs), integral to the tumour microenvironment, are pivotal in cancer progression, exhibiting either pro‐tumourigenic or anti‐tumourigenic functions. Their inherent phenotypic and functional diversity allows for subdivision of CAFs into various subpopulations. While several classification systems have been suggested different types, a unified molecular on single‐cell pan‐cancer scale has yet be established. Methods We employed comprehensive...
The incidence of papillary thyroid cancer (PTC) shows a predominance in females, with male:female ratio 1:3, and none the known risk factors are associated gender difference. Increasing evidence indicates role estrogen tumorigenesis, but mechanism involved remains largely unknown. This study aimed to assess contribution autophagy receptor α (ERα)-mediated growth PTC. expression ERα tissue patients PTC tissues was analyzed. Cell viability, proliferation, apoptosis were evaluated after...