A5066269765- Renal and related cancers
- TGF-β signaling in diseases
- Chronic Kidney Disease and Diabetes
- Toxic Organic Pollutants Impact
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Effects and risks of endocrine disrupting chemicals
- Mass Spectrometry Techniques and Applications
- Connective Tissue Growth Factor Research
- Pancreatic function and diabetes
- Per- and polyfluoroalkyl substances research
- Circular RNAs in diseases
- Metabolomics and Mass Spectrometry Studies
- Parathyroid Disorders and Treatments
- Sirtuins and Resveratrol in Medicine
- Liver Disease Diagnosis and Treatment
- Extracellular vesicles in disease
- Air Quality and Health Impacts
- Cardiac Fibrosis and Remodeling
- Birth, Development, and Health
- Gestational Diabetes Research and Management
- Amino Acid Enzymes and Metabolism
- Analytical Chemistry and Chromatography
- Fungal Biology and Applications
- GDF15 and Related Biomarkers
Chinese University of Hong Kong
2009-2022
Hong Kong Baptist University
2015-2021
Kowloon Hospital
2015-2020
Society of Environmental Toxicology and Chemistry
2020
Prince of Wales Hospital
2011-2017
Chinese University of Hong Kong, Shenzhen
2013-2014
University of Hong Kong
2008-2013
City University of Hong Kong, Shenzhen Research Institute
2012-2013
Kunming Institute of Botany
2013
Southern Medical University
2013
TGF-β/Smad3 signaling promotes fibrosis, but the development of therapeutic interventions involving this pathway will require identification and ultimate targeting downstream fibrosis-specific genes. In study, using a microRNA microarray real-time PCR, wild-type mice had reduced expression miR-29 along with progressive renal fibrosis in obstructive nephropathy. contrast, Smad3 knockout increased absence same model obstruction. cultured fibroblasts tubular epithelial cells, mediated...
TGF-β/Smad signaling plays a role in fibrogenesis, but therapies targeting TGF-β are ineffective treating renal fibrosis. Here, we explored the therapeutic potential of TGF-β-induced microRNA progression Microarray analysis and real-time PCR revealed upregulation miR-21 tubular epithelial cells (TECs) response to TGF-β. Lack Smad3, not lack Smad2, prevented from upregulating In addition, Smad3-deficient mice were protected fibrosis unilateral ureteral obstruction model. contrast, conditional...
Seed-chain-extend with k-mer seeds is a powerful heuristic technique for sequence alignment employed by modern aligners. While effective in practice both runtime and accuracy, theoretical guarantees on the resulting do not exist seed-chain-extend. In this work, we give first rigorous bounds efficacy of seed-chain-extend k-mers expectation. Assume are given random nucleotide length ≈ n that indexed (or seeded) mutated substring m ≤ mutation rate θ < 0.206. We prove can find k = Θ(log n)...
Smad2 and Smad3 interact mediate TGF-β signaling. Although promotes fibrosis, the role of in fibrogenesis is largely unknown. In this study, conditional deletion from kidney tubular epithelial cells markedly enhanced fibrosis response to unilateral ureteral obstruction. <i>In vitro</i>, knockdown increased expression collagen I, III, TIMP-1 decreased matrix-degrading enzyme MMP-2 TGF-β1 compared with similarly treated wild-type cells. We obtained similar results Smad2-knockout fibroblasts....
The main function of chemokines is to guide inflammatory cells in their migration sites inflammation. During the last 2 decades, an expanding number and receptors have driven broad inquiry into how are recruited a variety diseases. Although this review focuses on renal injury, proinflammatory IL-17, TGFβ, TWEAK signaling pathways also play critical role expression. Recent studies transgenic mice as well blockade chemokine by neutralizing ligands or receptor antagonists now allow direct...
// Shuang Wang 1, * , Xiao-Ming Meng Yee-Yung Ng 2, Frank Y. Ma 3 Zhou 1 Yang Zhang Chen Xiao-Ru Huang Jun Xiao Ying-Ying Shuk-Man Ka Yong-Jiang Tang Arthur C.K. Chung Ka-Fai To David J. Nikolic-Paterson Hui-Yao Lan Li Shing Institute of Health Sciences, Departments Medicine and Therapeutics, Chemical Pathology, Anatomical Cellular The Chinese University Hong Kong, Kong SAR, China 2 Division Nephrology, Department Medicine, Clinical Taipei Veterans General Hospital, National Ming University,...
Connective tissue growth factor (CTGF) plays a critical role in angiotensin II (Ang II)–mediated hypertensive nephropathy. The present study investigated the mechanisms and specific roles of individual Smads Ang II–induced CTGF collagen I expression tubular epithelial cells with deletion transforming (TGF)-β1, overexpression Smad7, or knockdown Smad2 Smad3. We found that mRNA protein expressions were regulated positively by phosphorylated Smad2/3 but negatively Smad7 because Smad7-abolished...
Although Smad3 has been considered as a downstream mediator of transforming growth factor-β (TGF-β) signaling in diabetes complications, the role Smad7 remains largely unclear. The current study tests hypothesis that may play protective and therapeutic potential for diabetic kidney disease.Protective disease was examined streptozotocin-induced mice have gene knockout (KO) rats given transfer using an ultrasound-microbubble-mediated technique.We found deficient developed more severe injury...
Advanced glycation end-products (AGEs) can induce expression of connective tissue growth factor (CTGF), which seems to promote the development diabetic nephropathy, but exact signaling mechanisms that mediate this induction are unknown. Here, AGEs induced CTGF in tubular epithelial cells (TECs) either lacked TGF-beta1 gene or expressed dominant TGF-beta receptor II, demonstrating independence TGF-beta. Furthermore, conditional knockout encoding II from kidney did not prevent AGE-induced...
Background. The present study tested the hypothesis that disruption of Smad7 function may accelerate renal fibrosis and inflammation.
(+)-Lingzhiol and (−)-lingzhiol, a pair of rotary door-shaped meroterpenoidal enantiomers, were isolated from Ganoderma lucidum. Their structures identified by spectroscopic methods X-ray diffraction crystallography. Lingzhiol bears an unusual 5/5/6/6 ring system characteristic sharing C-3′–C-7′ axis. Biological evaluation showed that (+)-lingzhiol or (−)-lingzhiol could selectively inhibit the phosphorylation Smad3 in TGF-β1-induced rat renal proximal tubular cells activate Nrf2/Keap1...
Blockade of transforming growth factor-β (TGF-β) signaling by Smad7 gene therapy is known to prevent experimental renal fibrosis. This study investigated whether suppresses fibrosis via altering the expression fibrosis-related microRNAs. Application into diseased kidneys obstructive nephropathy and kidney cells overexpressing restored miR-29b but inhibited miR-192 miR-21, resulting in blockade Furthermore, overexpression also suppressed advanced glycated end products- angiotensin...
Although Smad3 is a key mediator of fibrosis, the functional role in hypertensive cardiovascular disease remains unclear. The present study tested hypothesis that angiotensin II may activate transforming growth factor-beta/Smad3 pathway to mediate cardiac remodeling knockout (KO) and wild-type mice by subcutaneous infusion primary culture KO fibroblasts. Fourteen days after infusion, both developed equal levels high blood pressure. However, fibrosis inflammation were but not mice. This was...
Aristolochic acid nephropathy (AAN) has become a worldwide disease and is the most severe complication related to use of traditional Chinese medicine. However, pathogenic mechanisms AAN remain unclear therapies are limited. The present study tested hypothesis that transforming growth factor (TGF)-beta/Smad3 may be key pathway leading chronic AAN. This was examined in vivo Smad3 wild-type/knockout (WT/KO) mice vitro tubular epithelial cells with knockdown Smad2 or Smad3. Results revealed...
Despite the critical role that TGF-beta plays in renal fibrosis, transgenic mice overexpress human latent TGF-beta1 skin exhibit normal histology and function even though circulating levels of are an order magnitude higher than wild-type animals. In fact, seems to protect against inflammation a model ureteral obstruction. It is unknown, however, whether also has this effect immunologically mediated forms disease such as anti-GBM crescentic glomerulonephritis. We induced overexpressing...
C-reactive protein (CRP) is a risk factor or biomarker for cardiovascular diseases, including hypertension. The present study investigated the functional importance of human CRP in hypertensive cardiac remodeling by chronic infusion angiotensin II (Ang II) into mice that express CRP. Compared with wild-type mice, although Ang caused an equally high systolic blood pressure, levels were further elevated, and was markedly exacerbated CRP, resulting significant reduction left ventricular...