A5011364735- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Viral-associated cancers and disorders
- Cancer Genomics and Diagnostics
- Lymphoma Diagnosis and Treatment
- Sarcoma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- Metastasis and carcinoma case studies
- Glioma Diagnosis and Treatment
- MicroRNA in disease regulation
- Peptidase Inhibition and Analysis
- Tumors and Oncological Cases
- Lung Cancer Diagnosis and Treatment
- Liver physiology and pathology
- Cancer-related gene regulation
- Signaling Pathways in Disease
- NF-κB Signaling Pathways
- Epigenetics and DNA Methylation
- Lung Cancer Research Studies
- SARS-CoV-2 and COVID-19 Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Soft tissue tumor case studies
Chinese University of Hong Kong
2015-2024
Prince of Wales Hospital
2012-2024
University of Hong Kong
2005-2006
Activation of MET oncogene as the result amplification or activation mutation represents an emerging molecular target for cancer treatment. We comprehensively studied alterations and clinicopathologic correlations in a large cohort treatment-naïve non-small cell lung carcinoma (NSCLC).Six hundred eighty-seven NSCLCs were tested exon 14 splicing site (METΔ14), DNA copy number alterations, protein expression by Sanger sequencing, FISH, IHC, respectively.METΔ14 was detected 2.62% (18/687)...
Abstract Nasopharyngeal carcinoma (NPC) is an aggressive head and neck cancer characterized by Epstein-Barr virus (EBV) infection dense lymphocyte infiltration. The scarcity of NPC genomic data hinders the understanding biology, disease progression rational therapy design. Here we performed whole-exome sequencing (WES) on 111 micro-dissected EBV-positive NPCs, with 15 cases subjected to further whole-genome (WGS), determine its mutational landscape. We identified enrichment for aberrations...
As a distinct type of head and neck cancer, non-keratinizing nasopharyngeal carcinoma (NPC) is closely associated with EBV infection massive lymphoid infiltration. The unique histological features suggest that local inflammation plays an important role in NPC tumourigenesis. We comprehensively characterized NF-κB signalling, key inflammatory pathway which might contribute to the tumourigenesis this EBV-associated cancer. By EMSA, western blotting, immunohistochemical staining, constitutive...
Abstract Interplay between EBV infection and acquired genetic alterations during nasopharyngeal carcinoma (NPC) development remains vague. Here we report a comprehensive genomic analysis of 70 NPCs, combining whole-genome sequencing (WGS) microdissected tumor cells with oncogene expression to reveal multiple aspects cellular-viral co-operation in tumorigenesis. Genomic aberrations along EBV-encoded LMP1 underpin constitutive NF-κB activation 90% NPCs. A similar spectrum somatic viral gene...
Nasopharyngeal carcinoma (NPC) is a unique EBV-associated epithelial malignancy, showing highly invasive and metastatic phenotype. Despite increasing evidence demonstrating the critical role of cancer stem-like cells (CSCs) in maintenance progression tumors variety malignancies, existence properties CSC NPC are largely unknown. Our study aims to elucidate presence CSCs pathogenesis this malignant disease. Sphere-forming were isolated from an EBV-positive cell line C666-1 its tumor-initiating...
The role of oral microbiota in head and neck squamous cell carcinoma (HNSCC) is poorly understood. Here we sought to evaluate the association bacterial microbiome with host gene methylation patient outcomes, explore its potential as a biomarker for early detection or intervention. performed 16S rRNA amplicon sequencing sixty-eight HNSCC patients across both tissue rinse samples identify bacteria differential abundance between controls. A subset thirty-one pairs tumor tissues adjacent normal...
Abstract Background Cancer‐associated fibroblasts (CAFs), integral to the tumour microenvironment, are pivotal in cancer progression, exhibiting either pro‐tumourigenic or anti‐tumourigenic functions. Their inherent phenotypic and functional diversity allows for subdivision of CAFs into various subpopulations. While several classification systems have been suggested different types, a unified molecular on single‐cell pan‐cancer scale has yet be established. Methods We employed comprehensive...
Abstract Background Gastric cancer (GC) is one of the most common tumours in East Asia countries and associated with Helicobacter pylori infection. H. utilizes virulence factors, CagA VacA, to up‐regulate pro‐inflammatory cytokines activate NF‐κB signaling. Meanwhile, PIEZO1 upregulation cancer‐associated fibroblast (CAF) enrichment were found GC progression. However, mechanisms its involvement progression have not been fully elucidated. Methods The CAF clinical significance investigated...
Nasopharyngeal carcinoma (NPC) is an EBV-associated epithelial malignancy which prevalent in south-east Asia and southern China. Despite the multiple genetic epigenetic changes reported, contribution of dysregulated signalling pathways to this distinct type head neck cancer not well understood. Here we demonstrate up-regulation NOTCH ligands (JAG1 or DLL4) effector (HEY1) majority EBV-positive tumour lines primary tumours. Among receptors, NOTCH3 was over-expressed all 92.5% Aberrant...
Oncogenic driver mutations activating receptor tyrosine kinase pathways are promising predictive markers for targeted treatment. We investigated the mutation profile of an updated events list on kinase/RAS/PI3K axis and clinicopathologic implications in a cohort never-smoker predominated Chinese lung adenocarcinoma.We tested 154 adenocarcinomas adenosquamous carcinomas EGFR, KRAS, HER2, BRAF, PIK3CA, MET, NRAS, MAP2K1, RIT1 by polymerase chain reaction-direct sequencing. MET amplification...
SARS-CoV-2 transcribes a set of subgenomic RNAs (sgRNAs) essential for the translation structural and accessory proteins to sustain its life cycle. We applied RNA-seq on 375 respiratory samples from individual COVID-19 patients revealed that majority sgRNAs were canonical transcripts with N being most abundant (36.2%), followed by S (11.6%), open reading frame 7a (ORF7a; 10.3%), M (8.4%), ORF3a (7.9%), ORF8 (6.0%), E (4.6%), ORF6 (2.5%), ORF7b (0.3%); but ORF10 was not detected. The profile...
Nasopharyngeal carcinoma (NPC) is a distinct type of head and neck cancer which prevalent in southern China, south-east Asia northern Africa. The development stepwise progression NPC involves accumulation multiple gross genetic changes during the clonal expansion Epstein-Barr virus (EBV)-infected nasopharyngeal epithelial cell population. Here, using paired-end whole-transcriptome sequencing, we discovered number chimeric fusion transcripts panel EBV-positive tumour lines. Among these...
<b><i>Background:</i></b> The aim of current study was to (1) construct and validate a novel hepatocellular carcinoma (HCC)-specific inflammatory index; (2) compare the performances Integrated Liver Inflammatory Score (ILIS) existing 4 indices in HCC; (3) explore association between systemic/intratumoral markers. <b><i>Methods:</i></b> Two cohorts from Hong Kong (HK; <i>n</i> = 1,315) Newcastle (<i>n</i> 574) were...
Pulmonary lymphoepithelioma-like carcinoma (LELC) is a subtype of non-small cell lung cancer (NSCLC) characterized by marked lymphocytic infiltration and association with Epstein-Barr virus (EBV). The molecular basis underlying the disease remains unclear. We sought to study landscape multiple approaches including whole genomic sequencing, capture-based targeted fluorescent in situ hybridization immunohistochemistry. Tumor cells from 57 EBV-positive pulmonary LELCs were isolated careful...
Abstract Homeobox genes include HOX and non ‐HOX genes. proteins play fundamental roles during ontogenesis by interacting with other non‐ gene‐encoded partners performing transcriptional functions, whereas aberrant activation of family members drives tumorigenesis. In this study, gastric cancer (GC) expression microarray data indicated that HOXB9 is a prominent upregulated member in GC samples significantly associated clinical outcomes advanced TNM stages. However, the functional role...
Abstract Calcified chondroid mesenchymal neoplasms represent a distinct, and recently recognized, spectrum of tumors. To date most cases have been reported to be characterized by FN1 gene fusions involving multiple potential tyrosine kinase partners. Following incidental identification tumor morphologically corresponding calcified neoplasm, but with PDGFRA::USP8 fusion, we undertook retrospective review identify characterize additional such cases. A total four tumors were identified. Each...
Nasopharyngeal carcinoma (NPC) is a peculiar Epstein Barr virus (EBV)-associated malignancy that prevalent in South-East Asia. Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) isomerizes specific phosphorylated amino acid residues, which makes it an important regulator cell survival and apoptosis. In this study, we investigated the contribution made by PIN1 NPC tumorigenesis PIN1's potential role as therapeutic target.The expression of was examined panel lines, xenografts...