A5082362781- Glioma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Meningioma and schwannoma management
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Radiomics and Machine Learning in Medical Imaging
- Brain Metastases and Treatment
- Chromatin Remodeling and Cancer
- Single-cell and spatial transcriptomics
- Neurofibromatosis and Schwannoma Cases
- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- Drug Transport and Resistance Mechanisms
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Telomeres, Telomerase, and Senescence
- Histone Deacetylase Inhibitors Research
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- Innovations in Medical Education
- Genomics and Chromatin Dynamics
- Viral-associated cancers and disorders
- Advanced biosensing and bioanalysis techniques
Chinese University of Hong Kong
2014-2023
Prince of Wales Hospital
2006-2023
Hong Kong Association of Registered Tour Co-ordinators
2022
McMaster Children's Hospital
2017
Fudan University
2017
Chinese University of Hong Kong, Shenzhen
2013-2017
Huashan Hospital
2017
University of Hong Kong
2017
Fujita Health University Hospital
2013
Pitié-Salpêtrière Hospital
2013
Purpose BRAF V600E is a potentially highly targetable mutation detected in subset of pediatric low-grade gliomas (PLGGs). Its biologic and clinical effect within this diverse group tumors remains unknown. Patients Methods A combined genetic institutional study patients with PLGGs long-term follow-up was performed (N = 510). Clinical treatment data mutated PLGG (n 99) were compared large international independent cohort mutated-PLGG 180). Results 69 405 (17%) across broad spectrum histologies...
Astrocytoma of the isocitrate dehydrogenase (IDH) wild-type gene is described as a provisional entity within new World Health Organization (WHO) classification. Some groups believe that IDH lower-grade gliomas, when interrogated for other biomarkers, will mostly turn out to be glioblastoma. We hypothesize not all gliomas have very poor outcomes and group could substratified prognostically.Seven hundred eighteen adult WHO grades II III patients with from our hospitals were re-reviewed tested...
Single-cell multi-omics can provide a unique perspective on tumor cellular heterogeneity. Most previous single-cell whole-genome RNA sequencing (scWGS-RNA-seq) methods demonstrate utility with intact cells from fresh samples. Among them, many are not applicable to frozen samples that cannot produce suspensions. We have developed scONE-seq, versatile scWGS-RNA-seq method amplifies DNA and without separating them each other hence is compatible biobanked benchmarked scONE-seq against existing...
In the 2016, WHO classification of tumors central nervous system, isocitrate dehydrogenase (IDH) mutation is a main classifier for lower grade astrocytomas and IDH-mutated now regarded as single group with longer survival. However, molecular clinical heterogeneity among IDH mutant (WHO Grades II/III) have only rarely been investigated. this study, we recruited 160 astrocytomas, examined PDGFRA amplification, CDKN2A deletion CDK4 amplification by FISH analysis, TERT promoter Sanger sequencing...
Fibroblast growth factors (FGFs) and their receptors are significant components during fundamental cellular processes. FGF18 plays a distinctive role in modulating the activity of both tumor cells microenvironment. This study aims to comprehensively investigate expression functional gastric cancer (GC) elucidate its regulatory mechanisms. The upregulation was detected seven out eleven (63.6%) GC cell lines. In primary samples, overexpressed genomically stable chromosomal instability subtypes...
Abstract Adult medulloblastomas are clinically and molecularly understudied due to their rarity. We performed molecular grouping, targeted sequencing, TERT promoter Sanger sequencing on a cohort of 99 adult medulloblastomas. SHH made up 50% the cohort, whereas Group 3 (13%) was present in comparable proportion WNT (19%) 4 (18%). In contrast paediatric medulloblastomas, groups had no prognostic impact our ( p = 0.877). Most frequently mutated genes were (including mutations, 36% cases),...
// Rui-qi Zhang 1, 2, 4, * , Zhifeng Shi Hong Chen 5 Nellie Yuk-Fei Chung 2 Zi Yin Kay Ka-Wai Li Danny Tat-Ming Chan 3 Wai Sang Poon Jinsong Wu 4 Liangfu Zhou Aden Ka-yin Ying Mao Ho-Keung Ng 1 Department of Anatomical and Cellular Pathology, Chinese University Kong, China Shenzhen Research Institute, Neurosurgery Division, Surgery, Neurosurgery, Huashan Hospital, Fudan University, Shanghai, Neuropathology, These authors have contributed equally to this work Correspondence to: Chan, e-mail:...
// Aden Ka-Yin Chan 1, 2, * , Yu Yao 4, Zhenyu Zhang 4 Zhifeng Shi Liang Chen Nellie Yuk-Fei Chung 2 Joseph Shu-Ming Liu Kay Ka-Wai Li Danny Tat-Ming 3 Wai Sang Poon Ying Wang 5 Liangfu Zhou Ho-Keung Ng 1 Department of Anatomical and Cellular Pathology, Chinese University Hong Kong, Shatin, Kong Shenzhen Research Institute, The Shenzhen, China Neurosurgery Division, Surgery, Neurosurgery, Huashan Hospital, Fudan University, Shanghai, Neuropathology, These authors have contributed equally to...
IDH-mutant glioblastoma is classified by the 2016 CNS WHO as a group with good prognosis. However, actual number of cases examined in literature relatively small. We hypothesize that not uniform and should be further stratified.We conducted methylation profiles estimated copy variations 57 glioblastomas.Our results showed 59.6% 40.4% tumors belonged to glioma-CpG island methylator phenotype (G-CIMP)-high G-CIMP-low subgroups, respectively. subgroup was associated significantly worse overall...
MicroRNA-137 (miR-137) expression has been reported to be decreased in astrocytic tumors two profiling studies but its role the pathogenesis of oligodendroglial is still limited. In this study, we demonstrate that miR-137 significantly downregulated a cohort 35 and nine glioma cell lines compared with normal brains. Lower associated shorter progressive-free survival overall survival. Restoration an cells TC620, also glioblastoma U87 U373 suppressed growth, anchorage-independent as well...
Anatomy is a basic science for health professions curricula. Recent research suggests that the innovative blended learning approach (classroom plus use of online learning) outperforms conventional didactic teaching by facilitating effective learning. This study explores feasibility adopting in anatomy and evaluates experiences students. Courseware called electronic Professional Study (ePS) was developed used cardiovascular system non-medical ePS composed three condensed, recorded course...
Slit-Robo GTPase-activating protein 1 (SRGAP1) functions as a GAP for Rho-family GTPases and downstream of signaling. We aim to investigate the biological function SRGAP1 reveal its regulation by deregulated microRNAs (miRNAs) in gastric cancer (GC). mRNA expression were examined quantitative reverse transcription PCR (qRT-PCR) western blot. The role was demonstrated through siRNA-mediated knockdown experiments. miR-340 miR-124 confirmed blot, dual luciferase activity assays rescue is...
IDH mutations frequently occur in WHO grade II and III diffuse gliomas have favorable prognosis compared to wild-type tumors. However, whether predict enhanced sensitivity adjuvant radiation (RT) or chemotherapy (CHT) is still being debated. Recent studies identified recurrent the promoter region of telomerase reverse transcriptase (TERT) gliomas. We previously demonstrated that TERT may be promising biomarkers glioma survival prognostication when combined with mutations. This study analyzed...
Clonal evolution drives cancer progression and therapeutic resistance. Recent studies have revealed divergent longitudinal trajectories in gliomas, but early molecular features steering posttreatment remain unclear. Here, we collected sequencing clinical data of initial-recurrent tumor pairs from 544 adult diffuse gliomas performed multivariate analysis to identify predictors three glioma subtypes. We found that CDKN2A deletion at initial diagnosis preceded necrosis microvascular...
Recurrence and progression to higher grade lesions are characteristic behaviorsof gliomas. Though IDH1 mutation frequently occurs is considered as an early event in gliomagenesis, little known about its role the recurrence of We therefore analysed IDH2 statusat codon 132 172 by direct sequencing anti-IDH1-R132H immunohistochemistry 53 paired samples their recurrences, including 29 low- gliomas, 16 anaplastic gliomas 8 Glioblastomas. IDH1/IDH2 was detected 32 primarytumors, with 25 6...
Although 1p/19q codeletion is the genetic hallmark defining oligodendrogliomas, approximately 30-40% of oligodendroglial tumors have intact in literature and they demonstrate a worse prognosis. This group frequently suggested to be astrocytic nature with TP53 ATRX mutations but actually remains under-investigated. In present study, we provided evidence that not all are through histologic molecular approaches. We examined status by FISH large cohort 337 identified 39.8% lacking which was...