A5023674283- Glioma Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- MicroRNA in disease regulation
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Urological Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Brain Tumor Detection and Classification
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroblastoma Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Lipid Membrane Structure and Behavior
- CAR-T cell therapy research
- Urologic and reproductive health conditions
- Renal cell carcinoma treatment
- Pediatric Urology and Nephrology Studies
- Ureteral procedures and complications
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Neurological Disease Mechanisms and Treatments
- Genital Health and Disease
- Antioxidants, Aging, Portulaca oleracea
Huashan Hospital
2015-2024
Fudan University
2015-2024
Air Force Medical University
2016-2024
State Key Laboratory of Medical Neurobiology
2020-2024
Shanghai Center for Brain Science and Brain-Inspired Technology
2023-2024
Tang Du Hospital
2023-2024
Shanghai Clinical Research Center
2023
Xi'an Medical University
2022
Nanchang University
2015-2020
Shanghai Medical College of Fudan University
2013-2019
Cushing's disease, also known as adrenocorticotropic hormone (ACTH)-secreting pituitary adenomas (PAs) that cause excess cortisol production, accounts for up to 85% of corticotrophin-dependent syndrome cases. However, the genetic alterations in this disease are unclear. Here, we performed whole-exome sequencing DNA derived from 12 ACTH-secreting PAs and matched blood samples, which revealed three types somatic mutations a candidate gene, USP8 (encoding ubiquitin-specific protease 8),...
Engineered extracellular vesicles (EVs) are considered excellent delivery vehicles for a variety of therapeutic agents, including nucleic acids, proteins, drugs, and nanomaterials. Recently, several studies have indicated that clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) delivered by EVs enable efficient DNA editing. However, an RNA editing tool is still unavailable. Here, signal peptide-optimized EVs-delivered guide (gRNA) CRISPR/CasRx...
The morphology and surroundings of cells have been routinely used by pathologists to diagnose the pathological subtypes gliomas assess malignancy tumors. Thanks advent development digital pathology technology, it is possible automatically analyze whole slides tissue focus on nucleus in order derive a quantitative assessment that can be for grading, classification, diagnosis. During process computer-assisted diagnosis, accurate location segmentation nuclei from hematoxylin eosin (H&E)-stained...
To maintain brain homeostasis, a unique interface known as the blood-brain barrier (BBB) is formed between blood circulation and central nervous system (CNS). Major facilitator superfamily domain-containing 2a (Mfsd2a) specific marker of BBB. However, mechanism by which Mfsd2a influences BBB poorly understood. In this study, we demonstrated that essential for sphingosine-1-phosphate (S1P) export from endothelial cells in brain. We found Spinster homolog 2 (Spns2) form protein complex to...
Polystyrene nanoparticles (PS-NPs) are organic pollutants that widely detected in the environment and organisms, posing potential threats to both ecosystems human health. PS-NPs have been proven penetrate blood-brain barrier increase incidence of neurodegenerative diseases. However, information relating pathogenic molecular mechanism is still unclear. This study investigated neurotoxicity regulatory mechanisms neuroblastoma SHSY-5Y cells. The results show caused obvious mitochondrial...
Background: Stress-related obesity might be related to the suppression of hypothalamic-pituitary-adrenocortical axis and dysregulation metabolic system.Chronic stress also induces reward system increases risk food addiction, according recent clinical findings.However, few studies have tested effect chronic on addiction in animal models.Purpose: The objective this study was identify whether promotes or not explore possible mechanisms.Method: We applied adaily 2 hrsflashing LED irradiation...
Tumor location served as an important prognostic factor in glioma patients was considered to postulate molecular features according cell origin theory. However, anatomic distribution of unique subtypes not widely investigated. The relationship between phenotype and histological subgroup were also vague based on tumor location. Our group focuses the study distinctive subgroups histology subtypes, explores possibility their consistency clinical background. We retrospectively reviewed 143 cases...
Angiogenesis is a necessary process for solid tumor growth. Cellular markers endothelial cell proliferation are potential targets identifying the vasculature of tumors in homeostasis. Here we customize behaviors engineered cells to recognize Apj, surface marker neovascular endothelium, using synthetic Notch (synNotch) receptors. We designed apelin-based synNotch receptors (AsNRs) that can specifically interact with Apj and then stimulate pathways. Cells AsNRs have ability sense (ECs)....
Gliomas are the most common and recalcitrant intracranial tumors, approximately a quarter of which classified as lower-grade gliomas (WHO II–III). Although prognosis (LGGs) is significantly better than that higher-grade gliomas, highly heterogeneous tumor type, LGGs varies greatly based on molecular diagnosis. IDH wild-type used to be regarded dismal prognostic biomarker in LGGs; however, several studies revealed might not always equivalent glioblastoma IV). Hence, we hypothesize underlying...
BackgroundMolecular diagnosis is crucial for biomarker-assisted glioma resection and management. However, some limitations of current molecular diagnostic techniques prevent their widespread use intraoperatively. With the unique advantages ultrasound, this study developed a rapid intraoperative method based on ultrasound radio-frequency signals.MethodsWe built brain tumor bank with 169 cases enrolled since July 2020, which 43483 RF signal patches from 67 pathological were retrospective...
The blood–brain barrier (BBB) and drug resistance present challenges for chemotherapy of glioblastoma (GBM). A microneedle (MN) patch with excellent biocompatibility biodegradability was designed to bypass the BBB release temozolomide (TMZ) PLCG1-siRNA directly into tumor site synergistic treatment GBM. codelivery TMZ enhanced DNA damage apoptosis. potential mechanism behind this enhancement is knockdown PLCG1 expression, which positively regulates expression signal transducer activator...
Abstract Background Intracerebral hemorrhage (ICH) is a devastating neurological disease causing severe sensorimotor dysfunction and cognitive decline, yet there no effective treatment strategy to alleviate outcomes of these patients. The Mas axis-mediated neuroprotection involved in the pathology various diseases, however, role receptor setting ICH remains be elucidated. Methods C57BL/6 mice were used establish model by injection collagenase into striatum. agonist AVE0991 was administered...
Fewer than 5 % glioblastoma (GBM) patients survive over five years and are termed long-term survivors (LTS), yet their molecular background is unclear. The present cohort included 72 isocitrate dehydrogenase (IDH)-wildtype GBM patients, consisting of 35 LTS 37 short-term (STS), we employed whole exome sequencing, RNA-seq DNA methylation array to delineate this largest date. Although STS demonstrated analogous clinical characters classical biomarkers, CASC5 (P = 0.002) SPEN 0.013) mutations...
MicroRNA-543 (miR-543) has been suggested as an important regulator of the development and progression various cancer types. However, role biological function miR-543 in clear cell renal carcinoma (ccRCC) remains unclear. Here, we found that expression was significantly increased tumor tissues from ccRCC patients lines. We overexpression markedly promoted proliferation invasion cells, whereas suppression had opposite effects. Krüppel-like factor 6 (KLF6) identified a target gene miR-543....
Radiosensitivity of prostate cancer (PCa) cells promotes the curative treatment for PCa. The present study was designed to investigate synergistic effect genistein and AG1024 on radiosensitivity PCa cells. optimal X‑irradiation dose (4 Gy) concentration (30 µM) were selected by using CCK‑8 assay. Before Gy), PC3 DU145 treated with µM), (10 their combination. All treatments significantly reduced cell proliferation enhanced apoptosis. Using flow cytometric analysis, we found that arrested...
Direct reprogramming of human fibroblasts into functional neurons in vitro by defined factors provides an invaluable resource for regenerative medicine. However, clinical applications must consider the risk immune rejection, thus patient-specific induced neuronal cells (iNCs) may serve as ideal source autologous cell replacement. In this study, we report a robust process from patients' scalp lentiviral gene delivery Ascl1, Myt1l, and Sox2. These three-factor iNCs are similar to morphology,...
Although 1p/19q codeletion is the genetic hallmark defining oligodendrogliomas, approximately 30-40% of oligodendroglial tumors have intact in literature and they demonstrate a worse prognosis. This group frequently suggested to be astrocytic nature with TP53 ATRX mutations but actually remains under-investigated. In present study, we provided evidence that not all are through histologic molecular approaches. We examined status by FISH large cohort 337 identified 39.8% lacking which was...