A5011760131- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Viral-associated cancers and disorders
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Metastasis and carcinoma case studies
- Circular RNAs in diseases
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Genetic factors in colorectal cancer
- Lymphoma Diagnosis and Treatment
- Kruppel-like factors research
- Cancer Cells and Metastasis
- Lung Cancer Research Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Medical Imaging and Pathology Studies
- Colorectal Cancer Treatments and Studies
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Ferroptosis and cancer prognosis
- Mechanisms of cancer metastasis
- Immune cells in cancer
Chinese University of Hong Kong
2013-2023
Prince of Wales Hospital
2007-2023
Sun Yat-sen University Cancer Center
2023
Sun Yat-sen University
2023
Air Force Medical University
2011
National Institutes of Health
2011
Xijing Hospital
2011
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2011
University of Hong Kong
2000-2006
Hong Kong University of Science and Technology
2000
Epstein-Barr virus (EBV) is a herpesvirus associated with nasopharyngeal carcinoma (NPC), gastric (GC), and other malignancies. EBV the first human found to express microRNAs (miRNAs), functions of which remain largely unknown. We report on regulation cellular protein named p53 up-regulated modulator apoptosis (PUMA) by an miRNA known as miR-BART5, abundantly expressed in NPC EBV-GC cells. Modulation PUMA expression miR-BART5 anti–miR-BART5 oligonucleotide was demonstrated EBV-positive In...
Activation of MET oncogene as the result amplification or activation mutation represents an emerging molecular target for cancer treatment. We comprehensively studied alterations and clinicopathologic correlations in a large cohort treatment-naïve non-small cell lung carcinoma (NSCLC).Six hundred eighty-seven NSCLCs were tested exon 14 splicing site (METΔ14), DNA copy number alterations, protein expression by Sanger sequencing, FISH, IHC, respectively.METΔ14 was detected 2.62% (18/687)...
Epstein–Barr virus (EBV) was the first human found to encode microRNAs (miRNAs), but function of these miRNAs has been obscure. Nasopharyngeal carcinoma (NPC) is associated with EBV infection, and EBV-encoded LMP1 believed be a key factor in NPC development. However, detection protein variable. Here, we report that BART target 3′ UTR gene negatively regulate expression. These also modulate LMP1-induced NF-κB signaling alleviate cisplatin sensitivity LMP1-expressing cells. Consistent previous...
Yes-associated protein 1 (YAP1) is a multifunctional that can interact with different transcription factors to activate gene expression. The role of YAP1 in tumorigenesis unclear. We aimed investigate the functional gastric cancer.YAP1 expression adenocarcinoma was evaluated. biological function determined by proliferation assay, colony formation, cell invasion, and flow cytometric analysis through knocking down or ectopic expressing cancer lines coupled vivo study. possible downstream...
Infection with the Epstein-Barr virus (EBV) is a strong predisposing factor in development of nasopharyngeal carcinoma (NPC). Many viral gene products including EBNA1, LMP1, and LMP2 have been implicated NPC tumorigenesis, although de novo control these oncoproteins remains largely unclear. The recent discovery EBV-encoded microRNA (miRNA) lymphoid malignancies has prompted us to examine NPC-associated EBV miRNA. Using large-scale cloning analysis on EBV-positive cells, two novel miRNA, now...
Abstract BACKGROUND: Aberrant methylation of tumor‐related genes has been reported in Epstein‐Barr virus (EBV)‐associated gastric cancers. This study sought to profile EBV‐driven hypermethylation EBV‐infected cells. METHODS: The EBV‐positive AGS cancer cell line (AGS‐EBV) and EBV‐negative cells were used this study. DNA methyltransferase‐3b (DNMT3b) activity was assessed by EpiQuick assay, genome‐wide profiles methyl‐DNA immunoprecipitation microarray assay. RESULTS: EBV infection confirmed...
Abstract miR-375 is a tumor-suppressive microRNA (miRNA) in gastric cancer (GC). However, its molecular mechanism remains unclear. The aim of this study to comprehensively investigate how involved Hippo pathway by targeting multiple oncogenes. expression cell lines and primary GC was investigated qRT-PCR. regulation YAP1, TEAD4, CTGF evaluated qRT-PCR, western blot, luciferase reporter assays, respectively. functional roles the related genes were examined siRNA-mediated knockdown or ectopic...
MicroRNAs (miRNAs) have been reported to play an important role in tumorigenesis. In this study, the of miR-15a and miR-16-1 gastric adenocarcinoma (GAC) was investigated. The expression cell lines primary tumors examined by miRNA qRT-PCR. Proliferative assays, colony formation, invasion migration, flow cytometry analysis vivo study were performed ectopic miR-16-1. putative target genes explored TargetScan further validated. We found that down-regulated GAC tumor samples compared with normal...
Hepatocellular carcinomas (HCC) exhibit distinct promoter hypermethylation patterns, but the epigenetic regulation and function of transcriptional enhancers remain unclear. Here, our affinity- bisulfite-based whole-genome sequencing analyses reveal global enhancer hypomethylation in human HCCs. Integrative epigenomic characterization further pinpoints a recurrent hypomethylated CCAAT/enhancer-binding protein-beta (C/EBPβ) which correlates with C/EBPβ over-expression poorer prognosis...
Stathmin1 (STMN1) is a candidate oncoprotein and prognosis marker in several kinds of cancers. This study was aimed to analyze its expression biological functions gastric cancer. The STMN1 evaluated by qRT-PCR, western blot immunohistochemistry. function determined MTT proliferation assays, monolayer colony formation cell invasion assays using small interference RNA technique cancer lines. We also explored the regulation microRNA-223. upregulated lines primary adenocarcinomas. STMN1-positive...
In our previous whole-transcriptome sequencing analysis, downregulation of a long non-coding RNA, maternally expressed gene 3 (MEG3), was identified in NPC samples. This finding suggests the possible role MEG3 as tumor suppressor this distinctive disease. present study, two variants, AF119863 (MEG3-AF) and BX247998 (MEG3-BX), were found abundantly normal nasopharyngeal epithelial cell line, NP69. Significant MEG3-AF further verified panel samples including xenografts primary biopsies. is an...
Patients with colorectal cancer (CRC) have a high incidence of regional and distant metastases. Although metastasis is the main cause CRC-related death, its molecular mechanisms remain largely unknown. Using array-CGH expression microarray analyses, changes in DNA copy number mRNA levels were investigated human CRC samples. The level RASAL2 was validated by qRT-PCR, protein evaluated western blot as well immunohistochemistry cell lines primary tumors. functional role determined MTT...
Abstract Nasopharyngeal carcinoma (NPC) is closely associated with Epstein–Barr virus (EBV) infection. The EBV‐encoded latent membrane protein 1 (LMP1), which commonly expressed in NPC, engages multiple signaling pathways that promote cell growth, transformation, and metabolic reprogramming. Here, we report a novel function of LMP1 promoting de novo lipogenesis. increases the expression, maturation activation sterol regulatory element‐binding (SREBP1), master regulator lipogenesis, its...
Abstract Background Cancer‐associated fibroblasts (CAFs), integral to the tumour microenvironment, are pivotal in cancer progression, exhibiting either pro‐tumourigenic or anti‐tumourigenic functions. Their inherent phenotypic and functional diversity allows for subdivision of CAFs into various subpopulations. While several classification systems have been suggested different types, a unified molecular on single‐cell pan‐cancer scale has yet be established. Methods We employed comprehensive...
Human Disabled-2 (DAB2), is a multi-function signalling molecule that it frequently down-regulated in human cancers. We aimed to investigate the possible tumour suppressor effect of DAB2 nasopharyngeal carcinoma (NPC). studied expression NPC cell lines, xenografts and primary samples. The status promoter methylation was assessed by specific PCR bisulfite sequencing. functional role investigated re-introducing into line C666-1. Decrease or absent transcript observed lines xenografts. Loss...
Background The biological pathways and functional properties by which misexpressed microRNAs (miRNAs) contribute to liver carcinogenesis have been intensively investigated. However, little is known about the upstream mechanisms that deregulate miRNA expressions in this process. In hepatocellular carcinoma (HCC), hepatitis B virus (HBV) X protein (HBx), a transcriptional trans-activator, frequently expressed truncated form without carboxyl-terminus but its role expression HCC development...
KRAS mutational status has been shown to be a predictive biomarker of resistance anti-EGFR monoclonal antibody (mAb) therapy in patients with metastatic colorectal cancer. We report the spectrum mutation 1506 cancer and identification characterization rare insertion mutations within functional domain KRAS. are found 44.5% (670/1506) patients. Two cases harbor double involving both codons 12 13. The frequencies at its 12, 13, 61, 146 75.1%, 19.3%, 2.5%, 2.7%, respectively. most abundant codon...
Oncogenic driver mutations activating receptor tyrosine kinase pathways are promising predictive markers for targeted treatment. We investigated the mutation profile of an updated events list on kinase/RAS/PI3K axis and clinicopathologic implications in a cohort never-smoker predominated Chinese lung adenocarcinoma.We tested 154 adenocarcinomas adenosquamous carcinomas EGFR, KRAS, HER2, BRAF, PIK3CA, MET, NRAS, MAP2K1, RIT1 by polymerase chain reaction-direct sequencing. MET amplification...
Nasopharyngeal carcinoma (NPC) is a highly invasive epithelial malignancy that prevalent in southern China and Southeast Asia. It consistently associated with latent Epstein-Barr virus (EBV) infection. In NPC, miR-BARTs, the EBV-encoded miRNAs derived from BamH1-A rightward transcripts, are abundantly expressed contribute to cancer development by targeting various cellular viral genes. this study, we establish comprehensive transcriptional profile of panel NPC patient-derived xenografts an...
Nasopharyngeal carcinoma (NPC) is a distinct type of head and neck cancer which prevalent in southern China, south-east Asia northern Africa. The development stepwise progression NPC involves accumulation multiple gross genetic changes during the clonal expansion Epstein-Barr virus (EBV)-infected nasopharyngeal epithelial cell population. Here, using paired-end whole-transcriptome sequencing, we discovered number chimeric fusion transcripts panel EBV-positive tumour lines. Among these...