A5079175092- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cancer Research and Treatments
- Genetic factors in colorectal cancer
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Bone and Dental Protein Studies
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Epigenetics and DNA Methylation
- Gastric Cancer Management and Outcomes
- Peptidase Inhibition and Analysis
- Lung Cancer Treatments and Mutations
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Protease and Inhibitor Mechanisms
- Garlic and Onion Studies
- Trace Elements in Health
- Virus-based gene therapy research
- Helicobacter pylori-related gastroenterology studies
- Bioinformatics and Genomic Networks
- DNA Repair Mechanisms
- Colorectal Cancer Treatments and Studies
Peking University Cancer Hospital
2016-2025
Peking University
2016-2025
Chinese PLA General Hospital
2008-2025
Molecular Oncology (United States)
1987-2025
Beijing Hospital
2000-2020
Ministry of Education
2013-2020
Dana-Farber Cancer Institute
2020
BGI Group (China)
2003-2018
State Key Laboratory of Molecular Oncology
2008-2013
Huazhong University of Science and Technology
2013
Abstract Purpose: Both CD44 and CD133 were reported as putative markers for isolating colorectal cancer stem cells (CSC). It remains to be resolved if both of these are functional importance CSC. Experimental Design: The expression in normal colonic tissues primary was assessed by immunohistochemistry a series 60 patients on tissue microarray sections. vitro clonogenic vivo tumorigenic assay applied measure CSC activities from the isolated patients. Lentiviral RNA interference used stably...
Although aerobic glycolysis (the Warburg effect) is a hallmark of cancer, key questions, including when, how, and why cancer cells become highly glycolytic, remain less clear. For largely unknown regulatory mechanism, rate-limiting glycolytic enzyme pyruvate kinase M2 (PKM2) isoform exclusively expressed in embryonic, proliferating, tumor cells, plays an essential role metabolism growth. Because the receptor tyrosine kinase/PI3K/AKT/mammalian target rapamycin (RTK/PI3K/AKT/mTOR) signaling...
Significance In a few milliliters of blood from cancer patient, one can isolate circulating tumor cells (CTCs). Originating the primary tumor, CTCs seed metastases, which account for majority cancer-related deaths. We demonstrate analyses whole genome single CTCs, are highly needed personalized treatment. discovered that copy number variations (CNVs), major genomic variations, specific to types, reproducible cell cell, and even patient patient. hypothesize CNVs at certain loci selected lead...
Helicobacter pylori infection is mostly a family-based infectious disease. To facilitate its prevention and management, national consensus meeting was held to review current evidence propose strategies for population-wide H. control management reduce the related disease burden.Fifty-seven experts from 41 major universities institutions in 20 provinces/regions of mainland China were invited modify statements using Delphi process grading recommendations assessment, development evaluation...
<h3>Background</h3> Characterisation of colorectal cancer (CRC) genomes by next-generation sequencing has led to the discovery novel recurrently mutated genes. Nevertheless, genomic data not yet been used for CRC prognostication. <h3>Objective</h3> To identify recurrent somatic mutations with prognostic significance in patients CRC. <h3>Method</h3> Exome was performed tumour tissues 22 CRC, followed validation 187 and pathway-related genes using targeted capture additional 160 cases....
STAT3 signaling plays the pivotal role in tumorigenesis through EZH2 epigenetic modification, which enhanced activity by increased tyrosine phosphorylation of STAT3. Here, another possible feedback mechanism and clinical significance were investigated gastric cancer (GC). STAT3, p-STAT3 (Tyr 705) expression examined 63 GC specimens with matched normal tissues IHC staining. also identified five cell lines using RT-PCR western blot analyses. protein was detected blotting. In order to...
Abstract Accumulating evidence shows that microRNAs, functioning as either oncogenes or tumour suppressors by negatively regulating downstream target genes are actively involved in initiation and progression, may be promising biomarkers therapy targets. Data mining through a microRNA chip database indicated let‐7c associated with metastasis. Here, we confirmed down‐regulation of primary cancer tissues was significantly metastases, advanced TNM stages poor survival colorectal patients....
Abstract Purpose: CD44 is of functional importance for tumor initiation and progression in colorectal cancer, but how this molecule benefits cancer cells from the microenvironment, especially tumor-associated macrophages (TAM), remains poorly defined. Experimental Design: In vivo tumorigenic assays were conducted to assess role murine TAMs tumorigenesis human cells. Both vitro osteopontin (OPN) expression levels examined by immunohistochemistry, quantitative PCR, Western blotting. Soft agar...
Gastric cancer is not a single disease, and its subtype classification still evolving. Next-generation sequencing studies have identified novel genetic drivers of gastric cancer, but their use as molecular classifiers or prognostic markers disease outcome has yet to be established. In this study, we integrated somatic mutational profiles clinicopathologic information from 544 patients previous genomic identify significantly mutated genes (SMG) with relevance. were classified into regular...
Abstract Genome-wide analysis of genomic signatures might reveal novel mechanisms for gastric cancer (GC) tumorigenesis. Here, we structural variations (SVs) and mutational via whole-genome sequencing 168 GCs. Our data demonstrates diverse models complex SVs operative in GC, which lead to high-level amplification oncogenes. We find varying proportion tandem-duplications (TDs) among individuals identify 24 TD hotspots involving well-established genes such as CCND1, ERBB2 MYC . Specifically,...
As a newly identified and characterized gene, p42.3 is associated with cell proliferation tumorigenicity. The expression of upregulated in human gastric cancer (GC), but its underlying mechanisms action are not well understood. MicroRNAs (miRNAs) known to play vital regulatory roles many cellular processes. Here we utilized bioinformatics experimental approaches investigate the relationship between miRNAs gene. We showed that miR-29a could repress at both mRNA protein levels via directly...
Abstract Cancer stem cells are implicated in tumor progression, metastasis, and recurrence, although the exact mechanisms remain poorly understood. Here, we show that expression of cellular prion protein (PrPc, PRNP) is positively correlated with an increased risk metastasis colorectal cancer. PrPc defines a subpopulation CD44-positive cancer contributes to metastatic capacity. PrPc+CD44+ displayed high liver capability, unlike PrPc−CD44+ cells, was inhibited by RNAi-mediated attenuation...
The gene, collagen triple helix repeat containing 1 (CTHRC1), has been reported to increase in several kinds of human solid cancers and is associated with tumor invasion metastasis. To date, the expression function CTHRC1 gastric cancer (GC) have not reported. aim this study was investigate levels regulatory transcription mechanisms GC. Immunohistochemical analysis revealed that markedly increased carcinoma compared normal mucosa, chronic atrophic gastritis, intestinal metaplasia (P < 0.05...
Abstract Background Increasing evidence suggests that cancer is a metabolic disease. Here, we investigated the potential role of fructose-1,6-bisphosphatase-2 (FBP2), enzyme catalyses hydrolysis fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate in glucose metabolism, gastric (GC) development. Results Our data indicated FBP2 was downregulated GC tissues (86.2%, 100/116), absent or low expression correlated with poor survival patients ( P = 0.019). Conversely, ectopic...
Accumulating evidence suggests that long noncoding RNAs (lncRNAs) play important roles in transcriptional regulation, whereas the extent to which lncRNAs also function at posttranscriptional level is less known. In present study, we report a lncRNA named MT1JP acts as tumor suppressor through mechanism. We found differentially expressed tissues by analyzing data from customized microarray applied 76 pairs of matched normal and cancer tissue samples. By associating with RNA-binding protein...
The treatment of pancreatic cancer frequently fails due to local recurrence and hepatic metastasis. Our previous study found that Gd@C82(OH)22 can suppress by inhibiting MMP-2/9 expression. In this study, we further explored the epigenetic mechanism in human suppressed tumor metastasis through down-regulation metastasis-associated protein 1 (MTA1), HDAC1, HIF-1α, up-regulation reversion-cysteine with Kazal motif (RECK). level acetylation was increased promoter region RECK gene after...
Papillary thyroid carcinoma (PTC) is the most common adult malignancy and often presents with multiple anatomically distinct foci within thyroid, known as multifocal papillary (MPTC). The widespread application of next-generation sequencing technologies in cancer genomics research provides novel insights into determining clonal relationship between tumours same gland. For eight MPTC patients, we performed whole-exome targeted region to identify non-synonymous point mutations gene...
Abstract In vitro models coupled with multimodal approaches are needed to dissect the dynamic response of local tumor immune microenvironment (TIME) immunotherapy. Here patient‐derived primary lung cancer organoids (pLCOs) generated by isolating cell clusters, including infiltrated cells. A function‐associated single‐cell RNA sequencing (FascRNA‐seq) platform allowing both phenotypic evaluation and scRNA‐seq at single‐organoid level is developed TIME individual pLCOs. The analysis 171 pLCOs...
Abstract Purpose: Recent studies have highlighted that the p53 codon 72 polymorphism plays a crucial role in modulating wild-type apoptotic capacity, and as such may influence response to chemotherapy. Thus, purpose of this study was investigate whether might pathologic neoadjuvant chemotherapy primary breast cancer. Experimental Design: One hundred ten operable cancer patients received anthracycline-based status analyzed by PCR-RFLP. Results: The distribution initial clinical stage, tumor...
Metallothionein 2A (MT2A) and nuclear factor-kappaB (NF-κB) are both involved in carcinogenesis cancer chemosensitivity. We previously showed decreased expression of MT2A IκB-α human gastric (GC) associated with poor prognosis GC patients. The present study investigated the effect diallyl trisulfide (DATS), a garlic-derived compound, docetaxel (DOC) on regulation relation to NF-κB cells.DATS attenuated signaling cells, resulting G2/M cell cycle arrest apoptosis, culminating inhibition...