A5000732290- Reproductive Biology and Fertility
- Effects and risks of endocrine disrupting chemicals
- Birth, Development, and Health
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Ovarian function and disorders
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Circadian rhythm and melatonin
- Growth Hormone and Insulin-like Growth Factors
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Reproductive Physiology in Livestock
- Sirtuins and Resveratrol in Medicine
- Hydrogen's biological and therapeutic effects
- Biomedical Ethics and Regulation
- Retinal Diseases and Treatments
- Virus-based gene therapy research
- Immune responses and vaccinations
- Plant Stress Responses and Tolerance
- Adenosine and Purinergic Signaling
- Sexual Differentiation and Disorders
- Endometrial and Cervical Cancer Treatments
- Renal and related cancers
- Antioxidant Activity and Oxidative Stress
Jilin Medical University
2014-2025
Shanxi Datong University
2020
University of Minnesota
2018-2020
University of Science and Technology of China
2020
Minneapolis Heart Institute Foundation
2018
Nanjing Maternity and Child Health Care Hospital
2018
Nanjing Medical University
2018
Jining Medical University
2015
Fujian Normal University
2015
Jilin University
2014
Age-related macular degeneration (AMD) is the leading cause of severe visual loss in aged people. Melatonin has been shown to have capacity control eye pigmentation and thereby regulate amount light reaching photoreceptors, scavenge hydroxyradicals protect retinal pigment epithelium (RPE) cells from oxidative damage. Therefore, it reasonable think that physiological decrease melatonin people may be an important factor RPE dysfunction, which a well known for initiation AMD. Our purpose...
Abstract This study is to investigate whether astaxanthin could alleviate the oxidative stress damages of follicles induced by BPA and improve development cultured oocytes. Compared with group, survival rate, antrum formation oocyte maturation rate adherence area D8 D10 BPA+Asta group were significantly higher. The estrogen progesterone in culture medium PCNA granulosa cells ERα levels malondialdehyde follicle medium, ROS oocytes, expression caspase 3 cathepsin B oocytes lower. However,...
We investigated the effect of melatonin on bisphenol A (BPA)-induced oxidative stress damage in testicular tissue and Leydig cells. Mice were gavaged with 50 mg/kg BPA for 30 days, concurrently, injected (10 20 mg/kg). cells treated 10 μmol/L melatonin. The morphology organ index testis epididymis observed calculated. sperm viability density determined. expressions receptor 1A luteinizing hormone receptor, levels malonaldehyde, antioxidant enzymes, glutathione, steroid synthases, aromatase,...
This study investigates the effects of astaxanthin on oxidative stress, mitochondrial function, and follicular development in mouse preantral follicles, with a focus involvement adenosine monophosphate-activated protein kinase (AMPK) signaling pathway. Astaxanthin (2.5 nM) significantly enhanced both antrum formation (from 85.96% control group to 94.38% group) maturation rates 79.15% 85.12%) oocytes (p < 0.05). From day 4 vitro culture, notably increased area follicle attachment 0.06 µm2...
Bisphenol A (BPA) is an established environmental endocrine disruptor and can interfere with the development of female germ cells. However, underlying mechanisms are still unclear. We investigated effects BPA on granulosa cell meiosis oocytes using in vitro culture system mouse preantral follicles. Preantral follicles from D14 ovary were treated 10 μg/mL for 11 days. The adherent area was measured. On D11, cumulus expansion observed. recovery rate calculated. Western blot detected P53,...
Vascular endothelial growth factor (VEGF) is vital in normal and abnormal angiogenesis the ovary, particularly during early development of corpus luteum ovary. However, molecular regulation expression VEGF luteal vivo remains to be fully elucidated. As mediated by hypoxia‑inducible (HIF)‑1α cells cultured vitro, determined our previous study, present study was performed confirm hypothesis that HIF‑1α induced then regulates VEGF‑dependent development/function vivo. This investigated using a...
The aim of the present study was to investigate potential protective effects new Wenshen Shengjing Decoction (new WSSJD; including Cornu Cervi Nippon Parvum, Panax ginseng, Cynomorium songaricum, Cistanche deserticola, Radix Astragali, Epimedium brevicornum and Angelica sinensis) on cyclosporine‑induced impairment testosterone synthesis spermatogenic apoptosis in mice. A total 90 adult male Kunming mice were divided into following 6 groups: Control (no intervention), dimethylsulfoxide (DMSO;...
The corpus luteum is a temporary endocrine structure in mammals that plays an important role the female reproductive cycle and formed from ruptured ovulated follicle with rapid angiogenesis.Vascular endothelial growth factor (VEGF) thought to be vital normal abnormal angiogenesis ovary, but molecular regulation of luteal VEGF expression during development vivo still poorly understood at present.Therefore, we examined whether hypoxia-inducible factor-1a (HIF-1a) induced regulates function...
The aim of this study was to investigate the effect and mechanism bone morphogenetic protein-6 (BMP-6) on growth maturation mouse follicles in vitro. Preantral isolated from mice were incubated with recombinant human BMP-6 (rhBMP-6) before analysis. expression detected by immunofluorescence western blot. Maturation oocytes observed microscopically. Estradiol (E2) progesterone (P4) levels measured enzyme-linked immunosorbent assay. Expression steroidogenesis-related genes reverse...
Abstract Objective Efficiency of preantral follicle culture in vitro is low and dependent on species, development stage, follicle‐stimulating hormone (FSH) concentration. Here, we optimized the system mice. Methods The primary follicles (PM follicles, 80‐100 μm diameter ) early secondary (ES 110‐130 diameter) isolated from 14‐day female mice were cultured mediums containing 10 mIU/mL or 100 r‐FSH. growth oocyte maturation observed. Estradiol (E2) was detected by ELISA. FSH receptor (FSHR),...
Abstract Interspecies nuclear transfer has already achieved success in several species, which shows great potential recovery and conservation of endangered animals. The study was conducted to establish an efficient system for vitro argali ( Ovis ammon )‐sheep embryo reconstruction via interspecies somatic cell (iSCNT). competence domestic sheep cytoplasts reprogram the adult fibroblast nuclei evaluated, effects enucleation methods donor passage state on development argali‐sheep cloned...
Background. New Wenshen Shengjing Decoction (NWSSJD), a traditional Chinese compound medicine, has significant effect on spermatogenesis disorder and can significantly improve sperm quality. Many components in NWSSJD induce epigenetic modifications of different types cells. It is not yet known whether they cause or early embryos. Objective. This study investigated the mouse embryonic development its regulation H3K4me3 Methods. Spermatogenesis was induced male mice with CPA...
Many ingredients in Wenshen Shengjing Decoction (WSSJD) can cause epigenetic changes the development of different types cells. It is not yet known whether they sperms or early embryos. Here, we investigated role WSSJD modifications embryos and embryo development. A mouse model with spermatogenesis disorders was established cyclophosphamide (CPA). administrated for 30 days. The male mice after treatment were mated female treated superovulation. rate each stage calculated. Immunofluorescence...
We have investigated the mechanism for embryo development block in vitro and to improve rate of golden hamster embryos vitro. Intracytoplasmic sperm injection (ICSI) technique was used produce ICSI embryos. The changes histone acetylation expression deacetylase related genes were analyzed by immunocytochemical staining real-time PCR both vivo Aged oocytes significantly increased oocyte spontaneous activation rate. In cultured suffered from severe M199TE medium. Expression 1 (HDAC1) decreased...
Bisphenol A (BPA) is a commonly used phenolic environmental estrogen. Long-term exposure of female mammalians to BPA can lead endocrine disorders, followed by the morphological and functional changes in ovary, uterus, vagina, oviducts. The interactions with various target molecules or tissues will cause different effects. To further elucidate effects on reproductive system, we review structure functions reproduction system after their possible mechanisms.
To determine the effects of bisphenol-A (BPA) on blastocyst development and implantation.According to completely randomized grouping method, 90 pregnant mice were divided into 100, 300, 600 mg/(kg·d)BPA groups control group. BPA-treated orally administered with BPA at concentrations 300 mg/(kg·d) from day 0.5 3.5 their pregnancy. Blastocyst implantation studied.In group, number sites rate significantly decreased. In no observed among inhibited embryo implantation. 4 was examined, findings...
Melatonin,an endocrine hormone synthesized by the pineal gland,plays an important role in reproduction.The growth and development of follicles is basis female mammalian fertility.Follicles have a high concentration melatonin.Melatonin receptors exist on ovarian granulosa cells,follicle cells,and oocytes.It regulates these cells maturation atresia follicles,affecting fertility.This paper reviews protective effects regulatory mechanisms melatonin follicles,granulosa oocytes makes outlook...
To verify whether FN1 and ZNF438 are the androgen receptor(AR) target genes in LNCaP-AI cells investigate effects of AR exerts on gene expression.ChIP-PCR agarose gel electrophoresis were performed to fact that acted gene. Subsequently, treated with dihydrotestosterone(DHT)and lentivirus transfection down-regulate expression. The expression mRNA protein levels analyzed by RT-qPCR Western blot.FN1 enrichment AR-ChIP group 7.274±0.290 6.843±0.078, significantly higher than IgG-ChIP 1.004±0.113...