A5000520938- Complement system in diseases
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- COVID-19 Clinical Research Studies
- Renal Diseases and Glomerulopathies
- SARS-CoV-2 and COVID-19 Research
- Blood groups and transfusion
- Renal Transplantation Outcomes and Treatments
- Erythropoietin and Anemia Treatment
- Biomarkers in Disease Mechanisms
- Immune Cell Function and Interaction
- Kawasaki Disease and Coronary Complications
- Erythrocyte Function and Pathophysiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hemoglobinopathies and Related Disorders
- Pancreatitis Pathology and Treatment
- Iron Metabolism and Disorders
- Apelin-related biomedical research
- Peptidase Inhibition and Analysis
- COVID-19 Impact on Reproduction
- Multiple Myeloma Research and Treatments
- Thermal Regulation in Medicine
- T-cell and B-cell Immunology
- Pancreatic and Hepatic Oncology Research
- Hemophilia Treatment and Research
Semmelweis University
2014-2025
Hungarian Research Network
2021-2024
Emory University
2023
Aflac (United States)
2023
Children's Healthcare of Atlanta
2023
Hungarian Academy of Sciences
2018-2021
Third Way
2016
Objectives Uncontrolled thromboinflammation plays an important role in the pathogenesis of coronavirus disease (COVID-19) caused by SARS-CoV-2 virus. Complement was implicated as key contributor to this process, therefore we hypothesized that markers complement profile, indicative for activation state system, may be related severity and mortality COVID-19. Methods In prospective cohort study samples 102 hospitalized 26 outpatients with PCR-confirmed COVID-19 were analyzed. Primary outcome...
Vaccinations against SARS-CoV-2 reduce the risk of developing serious COVID-19 disease. Monitoring spike-specific IgG subclass levels after vaccinations may provide additional information on specific humoral immune response. Here, we examined presence and antibody subclasses in health-care coworkers vaccinated with vector- (Sputnik, AstraZeneca) or mRNA-based (Pfizer-BioNTech, Moderna) vaccines unvaccinated patients. We found that vector-based elicited lower total than mRNA vaccines. The...
Introduction While complement is a contributor to disease severity in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, all three pathways might be activated by the virus. Lectin pathway activation occurs through different pattern recognition molecules, including mannan binding lectin (MBL), protein shown interact with SARS-CoV-2 proteins. However, exact role of and its key molecule MBL COVID-19 still not fully understood. Methods We therefore investigated two...
Endothelial and complement activation were both associated with immunothrombosis, a key determinant of COVID-19 severity, but their interrelation has not yet been investigated. We aimed to determine von Willebrand factor (VWF) antigen (VWF:Ag) concentration, VWF collagen binding activity (VWF:CBA), disintegrin metalloproteinase thrombospondin type 1 motif, member 13 (ADAMTS13) (ADAMTS13:Ac), ratios in hospitalized patients, investigate how these parameters constellation relate disease...
Hematopoietic stem cell transplantation (HSCT)–associated thrombotic microangiopathy (TA-TMA) is a multifactorial complication, and its prediction largely unresolved. Our aim was to analyze changes of complement profile after HSCT identify potential markers TA-TMA development. Thirty-three consecutive pediatric patients (9.6 ± 4.4 years old) who underwent allogeneic due malignant (n = 17) or nonmalignant 16) indications were included in this study. Graft-versus-host disease (GVHD) diagnosed...
The most commonly used markers to assess complement activation are split products that produced through of all three pathways and located downstream C3. In contrast, C4d derives from the cleavage C4 indicates either classical (CP) or lectin pathway (LP) activation. Although is perfectly able distinguish between CP/LP alternative (AP) activation, no well-established available differentiate early CP LP Active enzymes both (C1s/C1r for CP, MASP-1/MASP-2 LP) regulated by C1 esterase inhibitor...
Aim: To investigate the serum circulating DPP4 activity in patients with COVID-19 disease. Materials & methods: Serum samples from 102 hospitalized and 43 post-COVID-19 plasma donors 39 SARS-CoV-2 naive controls their medical data were used. Circulating activities according to different disease peak severity (WHO) groups at sampling assessed. Results: A significant decrease (p < 0.0001) was found study of higher severity. When assessed as a prognostic marker, logistic regression = 0.0023)...
Neutrophil extracellular traps (NETs) can stimulate thrombosis, and their degradation is decreased in several autoimmune disorders. It was recently reported that some patients with haemolytic uraemic syndrome (HUS) also fail to degrade NETs neutrophils from Shiga toxin-associated HUS are primed form NETs.We used a well-characterized cohort of 74 thrombotic microangiopathy (TMA) patients, subset providing follow-up samples, 112 age-matched controls investigate NET serum nuclease activity TMA...
Abstract Multisystem inflammatory syndrome in children (MIS-C) is a rare, life-threatening complication of severe acute respiratory coronavirus 2 (SARS-CoV-2) infection. MIS-C develops with high fever, marked inflammation and shock-like picture several weeks after exposure to, or mild infection SARS-CoV-2. Deep immune profiling identified activated macrophages, neutrophils, B-plasmablasts CD8 + T cells as key determinants pathogenesis together multiple markers. The disease rapidly responds...
Background: The acquired form of idiopathic thrombotic thrombocytopenic purpura (TTP) is an autoimmune disease, in which the underlying deficiency ADAMTS13 protease caused by autoantibodies, predominantly IgG isotype. Certain HLA-DR-DQ haplotypes were associated with risk developing TTP. Objectives: To investigate development ADAMTS13-specific antibody response during course we analyzed concentration, subclass distribution, and inhibitory potential anti-ADAMTS13 autoantibodies samples TTP...
Hematopoietic stem cell transplantation (HSCT)-associated thrombotic microangiopathy (TA-TMA) is a multifactorial complication. Complement dysregulation may play an important role in the pathogenesis of TA-TMA. Our previous observations suggested that early increase soluble C5b-9 (sC5b-9), before development other complications, can predict later The present study aims to validate our earlier findings independent cohort enrolling 67 pediatric patients who underwent allogeneic HSCT during...
Biomarkers for monitoring COVID-19 disease course are lacking. Study aim was to identify biomarkers associated with severity, survival, long-term outcome, and Long COVID. As excessive macrophages activation is a hallmark of complement key in this, we selected the following proteins involved these processes: PTX3, C1q, C1-INH, C1s/C1-INH, sMR. EDTA-plasma concentrations were measured 215 patients 47 controls using ELISA. sMR, C1s/C1-INH levels severity. PTX3 sMR also survival immune recovery....
Abstract Anti-A Disintegrin and Metalloproteinase with a ThromboSpondin type 1 motif, member 13 (ADAMTS13) autoantibodies cause severe ADAMTS13 deficiency in immune-mediated thrombotic thrombocytopenic purpura (iTTP). consists of metalloprotease (M), disintegrin-like (D) domain, 8 thrombospondin repeats (T1-T8), cysteine-rich (C), spacer (S), 2 CUB domains (CUB1-2). We recently developed high-throughput epitope mapping assay based on small, nonoverlapping fragments (M, DT, CS, T2-T5, T6-T8,...
Introduction As the global pandemic continues, new complications of COVID-19 in pediatric population have turned up, one them being hemolytic uremic syndrome (HUS), a complement-mediated thrombotic microangiopathy (CM-TMA) characterized by triad thrombocytopenia, microangiopathic anemia and acute kidney injury (AKI). With both multisystem inflammatory children (MIS-C) HUS sharing complement dysregulation as key factors, aim this case report is to highlight differences between these two...
Background Mortalin/GRP75 is a ubiquitous mitochondrial chaperone related to the cytosolic heat shock protein 70. It protects cells from various types of damages and senescence. Our goal was determine whether COVID-19 patients have circulating mortalin in their blood assess its prognostic value anticipating disease severity. Methods Mortalin determined by ELISA sera 83 enrolled study. Patients were categorized into 4 groups: critical who died (FATAL) or required intensive care survived...
Severe clinical manifestations of multisystem inflammatory syndrome in children (MIS-C) are associated with the dysregulation immune response following SARS-CoV-2 infection. Therefore, we analyzed levels 10 selected cytokines at admission to estimate disease severity and predict length hospitalization. In remission samples, these mediators were followed after intravenous immunoglobulin (IVIG) treatment before discharge.