A5071748004- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Nonlinear Optical Materials Research
- RNA and protein synthesis mechanisms
- Solid-state spectroscopy and crystallography
- Enzyme Structure and Function
- Crystallography and molecular interactions
- Plant Virus Research Studies
- Photoreceptor and optogenetics research
- Protein Structure and Dynamics
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Bacteriophages and microbial interactions
- Crystal structures of chemical compounds
- Biochemical and Molecular Research
- Genomics and Phylogenetic Studies
- Urinary Tract Infections Management
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Infections and bacterial resistance
- Virology and Viral Diseases
- Biosensors and Analytical Detection
- Mitochondrial Function and Pathology
- Glycosylation and Glycoproteins Research
- Plant and Fungal Interactions Research
Carilion Clinic
2014-2025
Virginia Tech
2014-2024
Biomedical Research Institute
2024
Carilion Roanoke Community Hospital
2024
Jefferson College of Health Sciences
2014-2019
Frederick National Laboratory for Cancer Research
1989-2015
National Cancer Institute
1991-2015
University of Virginia
2008
International Crops Research Institute for the Semi-Arid Tropics
2002
Indian Institute of Science Bangalore
1971-2000
In MRL mice, the mostly recessive lpr mutation results in both accumulation of CD4-, CD8-, CD3+ T cells lymphoid tissue and many features generalized autoimmune disease, including immune complex glomerulonephritis. To positionally clone analyze effects background genes, backcross offspring were examined from cross: (MRL/MpJ-lpr x CAST/Ei)F1 MRL/MpJ-lpr. The gene was found to be closely linked a mouse chromosome 19 marker defined by variation Fas restriction fragment. Our identified...
A prediction algorithm based on physical characteristics of the twenty amino acids and refined by comparison to proposed bacteriorhodopsin structure was devised delineate likely membrane-buried regions in primary sequences proteins known interact with lipid bilayer. Application method sequence carboxyl terminal one-third bovine rhodopsin predicted a helical hairpin structure. With use lipid-buried segments as well other membrane-bound proteins, hierarchical ranking their preferences be...
The crystal structure of rabbit muscle creatine kinase, solved at 2.35 Å resolution by X‐ray diffraction methods, clearly identified the active site with bound sulfates surrounded a constellation arginine residues. putative binding creatine, which is occupied sulfate group in this analysis, has been tentatively identified. dimeric interface enzyme held together small number hydrogen bonds.
Dry ashing and wet techniques are routinely used for preparing plant materials elemental analysis. The two generally give similar results the analysis of but differences have been observed in using methods elements such as calcium (Ca), iron (Fe), zinc (Zn). We made a study to compare dry triacid digestion procedures determining potassium (K), Ca, magnesium (Mg), Fe, manganese (Mn), copper (Cu), Zn sorghum rice samples having range concentration these elements. For sorghum, gave...
Three-dimensional crystal structures of the homologous retroviral proteinases from Rous sarcoma virus (RSV PR) and human immunodeficiency (HIV-1 are to a large extent similar bear close resemblance six known bilobal fungal mammalian aspartic proteinases. Systematic three-dimensional structural superpositions were carried out between eucaryotic Both enzymes found be similarly related their counterparts. The most strongly conserved parts correspond those regions in N- C-domains that by...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural stability of halophilic proteinsJ. K. Mohana Rao and Patrick ArgosCite this: Biochemistry 1981, 20, 23, 6536–6543Publication Date (Print):November 1, 1981Publication History Published online1 May 2002Published inissue 1 November 1981https://doi.org/10.1021/bi00526a004RIGHTS & PERMISSIONSArticle Views425Altmetric-Citations72LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since 2008 (both...
CsrA/RsmA homologs are an extensive family of ribonucleic acid (RNA)-binding proteins that function as global post-transcriptional regulators controlling important cellular processes such secondary metabolism, motility, biofilm formation and the production secretion virulence factors in diverse bacterial species. While direct messenger RNA binding by has been studied detail for some genes, it is anticipated there numerous additional, yet undiscovered, targets mediate its regulation. To...
Abstract Background Biofilms are three dimensional communities of microorganisms that encased in self-produced extracellular polymeric substances. play a key role device-associated infections, including catheter-associated urinary tract largely because they protect from standard antimicrobial therapies. Antisense synthetic peptide nucleic acids (PNAs) an emerging approach to inhibiting specific components gene expression. Several regulatory and motility genes relatively conserved across...
Abstract Background Wastewater-based epidemiology (WBE) detects and mitigates pathogen outbreaks by evaluating conditions in a specific community. Between January 2019 September 2022, Escherichia coli, Citrobacter freundii, Pseudomonas aeruginosa, Salmonella enterica comprised about one-third of the local health department’s reported healthcare-associated infections. This pilot study attempted to detect quantitatively assess prevalence these bacterial pathogens order report public data state...
Abstract Background Catheter-associated urinary infections (CAUTIs) are among the most common healthcare-associated in United States. The risk of CAUTI corresponds to duration catheterization; prolonged catheter uses impacts patient morbidity, mortality, and healthcare costs. Pseudomonas aeruginosa is a leading cause particularly adept at forming surface-associated biofilms that difficult treat. In present study, we developed coating using polydopamine (PD) embed gentamicin on surface....
<title>Abstract</title> Biofilms develop in sequential steps resulting the formation of three-dimensional communities microorganisms that are encased self-produced extracellular polymeric substances. play a key role device-associated infections, such as catheter-associated urinary tract infections (CAUTIs), because they protect from standard antimicrobial therapies. Current strategies to prevent biofilm catheter-related including prophylactic antibiotics and antibiotic-coated catheters, have...
The crystal structure of l ‐asparaginase from Erwinia chrysanthemi in the presence and absence ‐aspartate was determined at 1.8 Å resolution. Conserved residues a left‐handed crossover (a rare occurrence protein structures) link pairs dimers into catalytically active tetrameric form enzyme. ErA containing bound aspartic acid shows that this unusual strand connectivity is an essential part site architecture, responsible for releasing product enzymatic hydrolysis. orientation aspartate...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of the aspartic protease from Rous sarcoma retrovirus refined at 2-.ANG. resolutionMariusz Jaskolski, Maria Miller, J. K. Mohana Rao, Jonathan Leis, and Alexander WlodawerCite this: Biochemistry 1990, 29, 25, 5889–5898Publication Date (Print):June 26, 1990Publication History Published online1 May 2002Published inissue 26 June 1990https://pubs.acs.org/doi/10.1021/bi00477a002https://doi.org/10.1021/bi00477a002research-articleACS...
The yeast GDHl gene encodes NADP-dependent glutamate dehydrogenase.This was isolated by complementation of an Escherichia coli auxotroph.NADP-dependent dehydrogenase overproduced 6-10-fold in Saccharomyces cereuisiae bearing on a multicopy plasmid.The nucleotide sequence the 1362-base pair coding region and 5' 3' flanking sequences were determined.Transcription start sites located S1 nuclease mapping.Regulation not maintained when present plasmid.Protein secondary structure predictions...
Abstract The structure of a crystal complex recombinant human immunodeficiency virus type 1 (HIV‐1) protease with peptide‐mimetic inhibitor containing dihydroxyethylene isostere insert replacing the scissile bond has been determined. is Noa‐His‐Hch[CH(OH)CH(OH)]Vam‐Ile‐Amp (U‐75875), and its K i for inhibition HIV‐1 <1.0 nM (Noa = 1‐naphthoxyacetyl, Hch hydroxy‐modified form cyclohexylalanine, Vam valine, Amp 2‐pyridylmethylamine). refined to crystallographic R factor 0.169 at 2.0 Å...
Pseudomonas aeruginosa undergoes phase variation in the expression of phosphorylcholine (ChoP) epitope, a structure crucial for virulence several respiratory pathogens. In this study, ChoP analysis comparing organisms from acute and chronic infections revealed that at 37 degrees C was higher among strains infections. Coimmunoprecipitation experiments mass spectrometry demonstrated on protein elongation factor Tu. The presence surface confirmed by immunofluorescence flow cytometry intact...
A prediction algorithm, designed to detect lipid‐embedded helical regions in membrane proteins, was applied the amino acid sequence of a chloroplast thylakoid protein important photosynthesis. It is suggested that consists 7 transmembrane helices connected by exposed turn segments, similar current models for bacteriorhodopsin and bovine rhodopsin. This basic structural feature, 7‐helical bundle, may prove be shared many integral proteins.
<title>Abstract</title> Minimization of the use antimicrobials is a recommended strategy to reduce evolution and spread antibiotic resistance; however, challenges remain in assessing efficacy such interventions. Wastewater-based surveillance (WBS) provides promising means consider differences resistance profiles across communities as function We examined metagenomes sewage entering two neighboring wastewater treatment plants (WWTPs) serving university nearby community. compared gene (ARG)...
The predicted amino acid sequence of rbsA, a gene from the high affinity ribose transport operon (rbs) Escherichia coli K12, is homologous to products hi&', malK, and pstl3, components histidine, maltose, phosphate operons.The recent finding by Hobson et d.(Hobson, A.