In MRL mice, the mostly recessive lpr mutation results in both accumulation of CD4-, CD8-, CD3+ T cells lymphoid tissue and many features generalized autoimmune disease, including immune complex glomerulonephritis. To positionally clone analyze effects background genes, backcross offspring were examined from cross: (MRL/MpJ-lpr x CAST/Ei)F1 MRL/MpJ-lpr. The gene was found to be closely linked a mouse chromosome 19 marker defined by variation Fas restriction fragment. Our identified...

To investigate genetic factors in asthma and atopy, we sought allelic associations for 12 markers near candidate loci serum total, immunoglobulin E (IgE), bronchial hyperresponsiveness (BHR) to inhaled histamine 131 families comprising 685 individuals, selected randomly without regard atopy or asthma. Nonparametric linkage association analyses were performed with the Analysis of Lineage Association (NOPAR) program, parametric Complex Inheritance Diathesis Severity (COMDS) program on an...

A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse human chromosome 1. In situ hybridization mapped GMP-140 bands 21-24 consistent with chromosomal localization LHR. Gene linkage analysis in the indicated that these genes serum coagulation factor V (FV) all map region distal is syntenic 1, no crossovers identified...

The chemokine receptor CXCR3 and its ligands CXCL9, CXCL10 CXCL11 are involved in variety of inflammatory disorders including multiple sclerosis, rheumatoid arthritis, psoriasis sarcoidosis. Two alternatively spliced variants the human CXCR3-A have been described, termed CXCR3-B CXCR3-alt. Human binds CXCL10, as well an additional ligand CXCL4. In contrast, CXCR3-alt only CXCL11. We report that CXCL4 induces intracellular calcium mobilization Akt p44/p42 extracellular signal-regulated kinase...

We have previously shown that arginase inhibition alleviates hypertension associated with in a diabetic animal model. Here, we investigated the protective effect of on metabolic syndrome.Metabolic syndrome was induced rats by administration fructose (10% drinking water) for 12 weeks to induce vascular dysfunction. Three inhibitors (citrulline, norvaline and ornithine) were administered daily last 6 study before tail BP recorded conscious animals. Concentration response curves phenylephrine...

Our website uses cookies to enhance your experience. By continuing use our site, or clicking "Continue," you are agreeing Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications Network Open Cardiology Dermatology Health Forum Internal Medicine Neurology Oncology Ophthalmology Otolaryngology–Head & Neck Surgery Pediatrics Psychiatry Archives of (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning /...

An efficient test of deviation from Hardy–Weinberg frequencies with one degree freedom was applied to 44 marker loci in a genome scan, and 7 had significant excess apparent homozygotes (χ 2 (1) > 6) suggestive typing error. In this example evidence for linkage did not increase when outliers were censored. Statistical quality control is an essential part genotyping, the effect mistyping map error should be considered evaluating any scan.

The role of pro-inflammatory cytokines in an animal model allergic lung disease was examined by use interleukin-1 receptor antagonist (IL-1ra) and a specific bioassay for tumor necrosis factor (TNF). Ovalbumin-sensitized guinea pigs exhibit marked bronchial hyperreactivity (assessed airway responsiveness to intravenous histamine) pulmonary eosinophil accumulation bronchoalveolar lavage) 24 h after challenge with aerosolized antigen. Exposure animals aerosol IL-1ra (50 µg over 30 min)...

The reader is presumed to have a broad understanding of plastic surgical procedures and concepts. After studying this article, the participant should be able to: Physicians may earn 1 AMA PRA Category credit by successfully completing examination based on material covered in article. begins page 176. ASAPS members can also complete CME online logging onto Members-Only Web site (http://www.surgery.org/members) clicking "Clinical Education" menu bar. Little has been published about venous...

The production of chemokines by vascular smooth muscle cells (SMC) is implicated in the pathogenesis atherosclerosis, although factors regulating chemokine these are incompletely characterized. We describe differential stimulation interleukin‐(IL)‐8, monocyte chemoattractant protein (MCP)‐1 and regulated on activation normal T‐cell expressed secreted (RANTES) synthesis following treatment human SMC with IL‐1α or tumour necrosis factor α (TNFα). Under basal conditions, cultured release very...

The mouse W19H mutation is an x-ray-induced deletion of more than 2 centimorgans on chromosome 5 encompassing the white spotting W (encoded by Kit protooncogene), patch (Ph), and recessive lethal (l) loci. platelet-derived growth factor receptor alpha gene (PDGFRA) like encodes a transmembrane tyrosine kinase. By using mouse-Chinese hamster somatic cell hybrids haplotype analysis in interspecific backcross mice, Pdgfra was mapped to tight linkage with Kit. Hybridization PDGFRA probe DNAs...

While inadvertent perioperative hypothermia has received serious attention in many surgical specialties, few discussions of have been published the plastic surgery literature. This article reviews physiology thermoregulation, describes how both general and regional anesthesia alter normal thermoregulatory mechanisms, indicates risk factors particularly associated with hypothermia, discusses most effective current methods for maintaining normothermia. Hypothermia is typically defined as a...

The accumulation and activation of inflammatory cells has long been recognized as a key event in host defence tissue injury. Prior to 1987, leucocyte chemotaxis was attributed largely the local generation complement fragments, lipids such leukotriene B4, or bacterial-derived peptides. A number chemotactic activities biological fluids cell culture supernatants had also reported, but at that time these were often mistakenly assumed be caused by known cytokines interleukin (IL)-1. Two papers1,2...

The frequency of isolation Burkholderia cepacia from the sputum cystic fibrosis (CF) patients is increasing. Using human A549 lung epithelial cell line, we have investigated ability B. exoproducts to stimulate interleukin-8 (IL-8) release. Cell-free supernatants a panel CF clinical, non-CF and nonclinical isolates were found IL-8 release, with levels ranging 11.8 +/- 2.8 80.0 3.5 ng/ml. A similar pattern was seen at level mRNA. bioactivity confirmed by examining its effect on intracellular...