A5081210798- RNA Research and Splicing
- Protein Kinase Regulation and GTPase Signaling
- Neurobiology and Insect Physiology Research
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Blood Coagulation and Thrombosis Mechanisms
- Signaling Pathways in Disease
- Cellular transport and secretion
- Vitamin K Research Studies
- Enzyme Structure and Function
- Plant Molecular Biology Research
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
- Biochemical and Structural Characterization
- Chemical Synthesis and Analysis
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Protein Structure and Dynamics
- S100 Proteins and Annexins
- Plant and animal studies
- Insect behavior and control techniques
- Hypothalamic control of reproductive hormones
- Lipid metabolism and biosynthesis
- Supramolecular Self-Assembly in Materials
AGH University of Krakow
2017-2024
Wrocław University of Science and Technology
2011-2024
Helmholtz Zentrum München
2020
Saint Louis University
2016
The clotting factor prothrombin exists in equilibrium between closed and open conformations, but the physiological role of these forms remains unclear. As for other allosteric proteins, elucidation linkage molecular transitions function is facilitated by reagents stabilized each alternative conformations. form has been characterized structurally, little known about architecture that predominates solution under conditions. Using X-ray crystallography single-molecule FRET, we characterize a...
Human transthyretin (TTR) is a tetrameric protein transporting thyroid hormones and retinol. TTR neuroprotective factor sensor of oxidative stress which stability diminished due to mutations aging, leading amyloid deposition. Adverse environmental conditions, such as redox metal ion imbalances, induce destabilization the structure. We have previously shown that was disturbed by Ca2+ other factors, including DTT, led formation an intrinsic fluorophore(s) emitting blue light, termed deep...
Abstract Nucleoplasmins are a nuclear chaperone family defined by the presence of highly conserved N-terminal core domain. X-ray crystallographic studies isolated nucleoplasmin domains revealed β-propeller structure consisting set five monomers that together form stable pentamer. Recent on from Drosophila 39-kDa FK506-binding protein (FKBP39) and other chromatin-associated proteins showed analogous, nucleoplasmin-like (NPL) pentameric structures. Here, we report NPL domain full-length FKBP39...
Abstract The Drosophila melanogaster Germ cell-expressed protein (GCE) is a paralog of the juvenile hormone (JH) receptor - Methoprene tolerant (MET). Both proteins mediate JH function, preventing precocious differentiation during D. development. Despite that GCE and MET are often referred to as equivalent receptors, their functions not fully redundant show tissue specificity. belong family bHLH-PAS transcription factors. similarity primary structure limited defined bHLH PAS domains, while...
In this paper, we show that a hierarchical approach for the construction of nanofibrils based on α,β-peptide foldamers is rational method design novel self-assembled nanomaterials peptides. Incorporation trans-(1S,2S)-2-aminocyclopentanecarboxylic acid residue into outer positions model coiled-coil peptide led to formation helical foldamers, which was determined by circular dichroism (CD) and vibrational spectroscopy. The oligomerization state obtained peptides in water established...
Nucleobindin-2 (Nucb2) is a protein that has been suggested to play roles in variety of biological processes. Nucb2 contains two Ca2+/Mg2+-binding EF-hand domains separated by an acidic amino acid residue-rich region and leucine zipper. All these are located within the C-terminal half protein. At N-terminal half, also possesses putative Zn2+-binding motif. In our recent studies, we observed underwent Ca2+-dependent compaction formed mosaic-like structure consisting intertwined disordered...
Abstract Nesfatin-1 and -2 are produced from a reaction in which the N-terminus of human Nucleobindin-2 undergoes proteolytical processing. To date, and/or nesfatin-1 have only been shown to act as peptide hormones. On other hand, purpose nesfatin-2 remains unknown. Since Nucleobindin-2/nesfatin-1 is thought impact control wide range physiological processes, including energy homeostasis, neurodegenerative processes carcinogenesis, its ligands/interactions deserve special studies attention....
Abstract Background Nucleobindin-2 (Nucb2) and nesfatin-1 (N1) are widely distributed hormones that regulate numerous physiological processes, from energy homeostasis to carcinogenesis. However, the role of nesfatin-2 (N2), second product Nucb2 proteolytic processing, remains elusive. To elucidate relationship between structure function nesfatins, we investigated properties chicken human homologs N1, as well a fragment consisting N1 N2 conjoined in head-to-tail manner (N1/2). Results Our...
The primary objective of this study was to implement a hierarchical approach enhance the conformational stability selected group peptides by incorporating
Nucleobindin-2 (Nucb2) is a multidomain protein that, due to its structure, participates in many physiological processes. It was originally identified several regions of the hypothalamus. However, more recent studies have redefined and extended function Nucb2 far beyond initially observed role as negative modulator food intake.Previously, we described structurally divided into two parts: Zn2+-sensitive N-terminal half Ca2+-sensitive C-terminal half. Here, investigated structural biochemical...
Nucleobindin-2 is a multidomain protein. can be cleaved into three peptide products: nesfatin-1, nesfatin-2 and nesfatin-3. It has been also shown that both nesfatin-1 exhibit anorexigenic effect in rodents. In this review, we focused on systematic characteristic of Nucleobindin-2, its discussed possible mechanisms action. The first one associated with melanocortin system leptin independent. second – involves neurons produce orexigenic neuropeptide Y. This allowed integrating key findings...
Abstract The Drosophila melanogaster Germ cell-expressed protein (GCE) is a paralog of the juvenile hormone (JH) receptor - Methoprene tolerant (MET). Both proteins mediate JH function, preventing precocious differentiation during D. development. Despite that GCE and MET are often referred to as equivalent receptors, their functions not fully redundant show tissue specificity. belong family bHLH-PAS transcription factors. similarity primary structure limited defined bHLH PAS domains, while...
Abstract The Drosophila melanogaster Germ cell-expressed protein (GCE) is a paralog of the juvenile hormone (JH) receptor - Methoprene tolerant (MET). Both proteins mediate JH function, preventing precocious differentiation during D. development. Despite that GCE and MET are often referred to as equivalent receptors, their functions not fully redundant show tissue specificity. belong family bHLH-PAS transcription factors. similarity primary structure limited defined bHLH PAS domains, while...