Raman spectroscopy is highly sensitive to the morphology and electronic structures of graphitic materials, but a convenient interpretation model has been lacking for multiwalled carbon nanotubes (MWCNTs), in particular discrimination spectral changes induced by covalent functionalization. The present work describes systematic investigation analysis covalently functionalized MWCNTs diazonium chemistry oxidation methodologies, with typically different mechanisms reaction sites. A multi-peak...

Abstract Peptide foldamers containing both cis ‐β‐aminocyclopentanecarboxylic acid and α‐amino residues combined in various sequence patterns (ααβ, αααβ, αβααβ, ααβαααβ) were screened using CD NMR spectroscopy for the tendency to form helices. ααβ‐Peptides found fold into an unprecedented well‐defined 16/17/15/18/14/17‐helix. By extending length of or shifting a fragment from one terminus another ααβ‐peptides, balance between left‐handed right‐handed helix populations present solution can be...

The incorporation of cyclopentane-based beta-amino acid in the sequence peptide forming coiled-coil induced formation nanofibrils.

CsgA is an aggregating protein from bacterial biofilms, representing a class of functional amyloids. Its amyloid propensity defined by five fragments (R1–R5) the sequence, non-perfect repeats. Gate-keeper amino acid residues, specific to each fragment, define fragment’s for self-aggregation and characteristics whole protein. We study secondary structures repeat Salmonella enterica Escherichia coli comparatively analyze their potential effects on these proteins in biofilm. Using...

NLR proteins are intracellular receptors constituting a conserved component of the innate immune system cellular organisms. In fungi, NLRs characterized by high diversity architectures and presence amyloid signaling. Here, we explore diverse world effector signaling domains fungal using state-of-the-art bioinformatic methods including MMseqs2 for fast clustering, probabilistic context-free grammars sequence analysis, AlphaFold2 deep neural networks structure prediction. addition to...

Amyloid proteins are often associated with the onset of diseases, including Alzheimer's, Parkinson's and many others. However, there is a wide class functional amyloids that involved in physiological functions, e.g., formation microbial biofilms or storage hormones. Recent studies showed an amyloid fibril could affect aggregation another protein, even from different species. This may result amplification attenuation process. Insight into cross-interactions be crucial for better understanding...

An α-helical PSMα3 considerably inhibits the fibrillation of human insulin in vitro . The anti-amyloidogenic effect is concentration-dependent. aggregation through suppression primary nucleation.

In this paper, we show that a hierarchical approach for the construction of nanofibrils based on α,β-peptide foldamers is rational method design novel self-assembled nanomaterials peptides. Incorporation trans-(1S,2S)-2-aminocyclopentanecarboxylic acid residue into outer positions model coiled-coil peptide led to formation helical foldamers, which was determined by circular dichroism (CD) and vibrational spectroscopy. The oligomerization state obtained peptides in water established...

To unveil and shape the molecular connectivity in (metallo)porphyrin–carbon nanotube hybrids are of main relevance for multiple medicinal, photoelectronic, catalytic, photocatalytic applications these materials. Multi-walled carbon nanotubes (MWCNTs) were modified through 1,3-dipolar cycloaddition reactions with azomethine ylides generated situ carrying pentafluorophenyl groups, followed by immobilization β-amino-tetraphenylporphyrinate Zn(II). The functionalities confirmed via XPS FTIR,...

Amyloid signaling motifs are a class of protein which share basic structural and functional features despite the lack clear sequence homology. They hard to detect in large databases either with alignment-based profile methods (due short length diversity) or generic amyloid- prion-finding tools insufficient discriminative power). We propose address challenge machine learning grammatical model capable generalizing over diverse collections unaligned yet related motifs.First, we introduce test...

The primary objective of this study was to implement a hierarchical approach enhance the conformational stability selected group peptides by incorporating

Abstract Peptide foldamers containing both cis ‐β‐aminocyclopentanecarboxylic acid and α‐amino residues combined in various sequence patterns (ααβ, αααβ, αβααβ, ααβαααβ) were screened using CD NMR spectroscopy for the tendency to form helices. ααβ‐Peptides found fold into an unprecedented well‐defined 16/17/15/18/14/17‐helix. By extending length of or shifting a fragment from one terminus another ααβ‐peptides, balance between left‐handed right‐handed helix populations present solution can be...

The key issue in the research on foldamers remains understanding of relationship between monomers structure and conformational properties at oligomer level. In peptidomimetic foldamers, main goal which is to mimic proteins, a challenge still better folding peptides factors that influence their stability. We probed impact modification peptide periphery with trans- cis-2-aminocyclopentanecarboxylic acid (ACPC) stability model coiled-coil using circular dichroism (CD), analytical...

In this paper, we report the obtaining of foldameric peptides by incorporation trans-(1S,2S)-2aminocyclopentanecarboxylic acid residues (trans-ACPC) into model coiled-coil structures and preliminary studies on their propensity for encapsulation small molecules.The secondary structure obtained was elucidated using circular dichroism (CD), were tested as nanocarriers ligand -1,6-diphenylehexatriene (DPH) fluorescence studies.The experimental results supported molecular modelling studies.Two...

The CsgA protein is the most studied functional amyloid produced by numerous strains of Enterobacteriaceae. Although sequences are highly conserved, they exhibit species diversity, which reflects specific evolutionary and adaptability major curli subunit. To understand differences in amyloidogenic properties R4 fragments Escherichia coli Salmonella enterica, based on bioinformatic analysis, we proposed four mutants: M1, M2, M3, M4. mutated were characterized various experimental techniques,...

Abstract NLR proteins are intracellular receptors constituting a conserved component of the innate immune system multicellular organisms. In fungi, NLRs characterized by high diversity architectures and presence amyloid signaling. Here, we explore diverse world effector signaling domains fungal using state-of-the-art bioinformatic methods including MMseqs2 for fast clustering, probabilistic context-free grammars sequence analysis, AlphaFold2 deep neural networks structure prediction....

ABSTRACT Amyloids are protein aggregates usually associated with their contribution to several diseases e.g., Alzheimer’s and Parkinson’s. However, they also beneficially utilized by many organisms in physiological roles, such as microbial biofilm formation or hormone storage. Recent studies showed that an amyloid aggregate can affect aggregation of another protein. Such cross-interactions may be crucial for understanding the comorbidity influence amyloids on human amyloidogenic proteins....

Abstract Background Amyloid signaling motifs are a class of protein which share basic structural and functional features despite lack apparent sequence homology. They hard to detect in large databases either with the alignment-based profile methods (due short length diversity) or generic amyloid- prion-finding tools insufficient discriminative power). We propose address challenge machine learning grammatical model capable generalizing over diverse collections unaligned yet related motifs....