A5048659459- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- Endoplasmic Reticulum Stress and Disease
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2001-2020
Medical University of Warsaw
2001
Arabidopsis thaliana AtNUDT7 Nudix pyrophosphatase hydrolyzes NADH and ADP-ribose in vitro is an important factor the cellular response to diverse biotic abiotic stresses. Several studies have shown that loss-of-function Atnudt7 mutant plants display many profound phenotypes. However molecular mechanism of function remains elusive. To gain a better understanding this hydrolase role, proteins interacting with were identified. Using as bait binding assay derived from cultured cell extracts we...
Abstract Nudix proteins catalyze the hydrolysis of pyrophosphate bonds in a variety substrates and are ubiquitous all domains life. The genome an important opportunistic human pathogen, Pseudomonas aeruginosa , encodes multiple proteins. To determine role nine hydrolases P. PAO1161 strain its fitness, virulence or antibiotic resistance mutants devoid individual enzymes were constructed analyzed for growth rate, motility, biofilm formation, pyocyanin production, susceptibility to oxidative...
Summary The PA0336 protein from Pseudomonas aeruginosa belongs to the family of widely distributed Nudix pyrophosphohydrolases, which catalyze hydrolysis pyrophosphate bonds in a variety nucleoside diphosphate derivatives. amino acid sequence is highly similar that RppH RNA pyrophosphohydrolase Escherichia coli , removes 5′‐end triphosphorylated transcripts. Trans‐complementation experiments showed P. enzyme can functionally substitute for E. cells indicating that, RppH, hydrolase mediates...
Summary Nudix pyrophosphatases, ubiquitous in all organisms, have not been well studied. Recent implications that some of them may be involved response to stress and pathogenesis indicate they play important biological functions. We investigated NudC proteins from the plant pathogen P seudomonas syringae pv. tomato str. DC 3000 human aeruginosa PAO 1161. found these homologous enzymes are homodimeric vitro preferentially hydrolyse NADH . The mutant strain deficient accumulated displayed...