A5016610664- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2017-2020
Abstract Nudix proteins catalyze the hydrolysis of pyrophosphate bonds in a variety substrates and are ubiquitous all domains life. The genome an important opportunistic human pathogen, Pseudomonas aeruginosa , encodes multiple proteins. To determine role nine hydrolases P. PAO1161 strain its fitness, virulence or antibiotic resistance mutants devoid individual enzymes were constructed analyzed for growth rate, motility, biofilm formation, pyocyanin production, susceptibility to oxidative...
Summary The PA0336 protein from Pseudomonas aeruginosa belongs to the family of widely distributed Nudix pyrophosphohydrolases, which catalyze hydrolysis pyrophosphate bonds in a variety nucleoside diphosphate derivatives. amino acid sequence is highly similar that RppH RNA pyrophosphohydrolase Escherichia coli , removes 5′‐end triphosphorylated transcripts. Trans‐complementation experiments showed P. enzyme can functionally substitute for E. cells indicating that, RppH, hydrolase mediates...