Unique biosynthetic abilities revealed by plants determine in vitro cultures of hairy roots as a suitable source pharmaceutically relevant bioactive compounds. The basic aim the study was to examine applicability aerogel composed methyltrimethoxysilane (MTMS) for immobilization Rindera graeca identifying quantitative effects biomass proliferation and naphthoquinones extracellular secretion aerogel-supported culture system. R. were simultaneously cultured 28-days, (i) nonimmobilized...

Rocking single-use bioreactors (SUBs) adopt an effective mixing mechanism based on the rocking movement of a platform which disposable bag-like container is placed. Non-typical liquid flow patterns make hydrodynamical properties SUBs not yet well systematized. The aim study was to determine quantitative characteristic time in ReadyToProcess WAVE 25 system equipped with 2-litre Cellbag container, use sensor method and Design Experiment (DoE) methodology. DoE-based approach has been used...

Nanobubbles can enhance both the proliferation and metabolic activity of microorganisms (mainly bacteria) growth whole higher organisms such as mice, fish, or plants. The critical fact is that nanobubbles different gases affect given cells differently. As animal cell cultures are used in industry research studies, investigations their interactions with should be carried out. This study aims to uncover whether presence improves rate L929 fibroblasts HL60 leukemia exemplary lines adherent...

Wave mixed single-use bioreactors (also called rocking bioreactors) are a group of devices in which mixing is caused by characteristic wave-like movement liquid inside pillow-shaped bag mounted on continuously oscillating platform. This study aimed to investigate the influence phase viscosity and presence an antifoaming agent mass transfer 2 liter used ReadyToProcess WAVE 25 bioreactor system. The rate was quantified measuring volumetric liquid-side coefficient kLa using gassing-out method....

Abstract Growth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions 2-D wave-assisted agitation has been compared and discussed. Influence Reynolds number for liquid (Re L ) the k a coefficient, as key parameters characterized bioprocessing cells ReadyToProcess WAVE TM 25 system, on reached values apparent maximal specific growth rate ( μ max yield biomass Y * X/S identified. The Re (i.e., 510–10,208), well coefficient 2.83–13.55...

For millennia, plants have been a source of natural medicines used by humankind. In vitro cultures plant organs, e.g., transgenic roots, provide suitable environment for maintaining biomass and continuously producing plant-derived bioproducts. The in can be efficiently scaled up using disposable bioreactors with wave-type agitation conditions. study aimed to investigate the influence conditions supported WAVE 25 bioreactor on proliferation secondary metabolite production offered system...

The growth of CP5 chondrocytes on microcarriers beads in a disposable bioreactor under various conditions wave-assisted agitation has been compared and discussed. Influences Reynolds number for liquid (ReL) the kLa coefficient intensification rate (μ) specific yield biomass (Y*X/S) have identified. Culture system established ReadyToProcess WAVETM25 with 2-litre flexible bag-like container. Cytodex 3 identified as most suitable microcarrier fulfilled requirements. Based simplified...

In vitro plant cell and tissue culture systems allow for controlling a wide range of environmental factors selectively influencing biomass growth the yield secondary metabolites. Among most efficient methods, complex supplementation medium with elicitors, precursors, other functional substances may significantly enhance valuable metabolite productivity through stress induction mechanism. search novel techniques in experimental biotechnology, goal study was to evaluate stress-inducing...

Typically applied static (i.e.non-agitated) cultures do not provide sufficient conditions for efficient propagation of suspended non-adherent cells, in general.Feasibility small-scale wave-type agitated single-use bioreactors gentle agitation underlies applicability such systems scaling-up fragile biomass animal cells.The basic aim the study was to compare results HL-60 cell performed referentially as batch culture typical disposable flasks (50 cm 3 medium) and ReadyToProcess WAVE TM 25...

The research has been conducted to obtain scaffolds for cancellous bone regeneration. Polylactide were made by the phase inversion method with a freeze-extraction variant, including gelling polylactide in its non-solvent. Substitutes of are hydrophobic, which limits cell adhesion. For this reason, modified using chitosan and folic acid forming gel-like coatings on surface. modification aimed improve material’s surface properties increase Analyses obtained confirmed effectiveness performed...

In situ extraction is a method for separating plant secondary metabolites from in vitro systems of biomass cultures. The study aimed to investigate the MTMS-based xerogels morphology effect on growth kinetics and deoxyshikonin productivity xerogel-supported culture Rindera graeca hairy root. Cultures were supplemented with three types xerogel, i.e., mesoporous gel, microporous agglomerated precipitate, disintegrated or monolithic form. Structure, oil sorption capacity, SEM analyses...

Cells‐containing scaffolds made of biomaterial are modern hybrid constructs which allow regeneration or reconstruction damaged connective tissue, e.g. cartilage bone. The basic aim the study was to recognize impact scaffold production parameters (i.e. type applied pore precursor, its concentration) on morphology and mechanical properties polylactide (PLLA) membrane‐based constructs, as well their biocompatibility exhibited cellular response. Design experiments (DoE) strategy has been...

Secondary metabolites derived from plants are recognized as valuable products with several successful applications in the pharmaceutical, cosmetic, and food industries. The major limitation to broader implementation of these compounds is their low manufacturing efficiency. Current efforts overcome unprofitability depend mainly on biotechnological methods, especially through application plant vitro cultures. This concept allows unprecedented bioengineering opportunities for culture system...

Biomaterials containing citric acid as a building unit show potential for use blood vessel and skin tissue substitutes. The success in commercializing implants polymer matrix of poly(1,8-octanediol citrate) provides rationale exploring polycitrates based on other diols. Changing the aliphatic chain length diol allows functional design strategies to control implant's mechanical properties, degradation profile surface energy. In present work, poly(1,2-ethanediol was synthesized used an...

The study aims to approach a coating layer based on alginate functionalized with albumin nanocoated oxygenating factor, constituting safe and even contact surface epithelial cells in system components for medical devices support maintaining the human lung cells' function. incorporated albumin-coated element was designed (ALG_Alb-PFD). As an perfluorodecalin applied at three saturation levels oxygen: 0%, 21%, 100%. studies were performed adenocarcinoma A549 cell lines derived from cultured...

Polymers are of great interest for medical and cosmeceutical applications. The current trend is to combine materials natural synthetic origin in order obtain products with appropriate mechanical strength good biocompatibility, additionally biodegradable bioresorbable. Citric acid, being an important metabolite, interesting substance the synthesis biomedical Due high functionality molecule, it commonly used biomaterials chemistry as a crosslinking agent. Among citric acid-based biopolyesters,...

Systems of two immiscible liquid phases—aqueous phase (i.e., distilled water (dH2O) or phosphate-buffered saline (PBS)) and perfluorochemical perfluorodecalin (PFD))—were subjected to wave-assisted agitation, i.e., oscillatory rocked, in a disposable bag-like container ReadyToProcess WAVETM25 bioreactor, recognize oxygen transfer effects effectivity the surface aeration. According DoE methodology, values volumetric liquid-side mass (kLa) coefficient for dH2O, PBS, dH2O-PFD, PBS-PFD systems...

The aim of the study was to determine characteristics mixing time in a rocking singleuse bioreactor equipped with 2-litre bag-like container by sensor method.Results have shown that frequency movement has biggest influence on values time.Кеуwordssingle-use bioreactor, wave-type agitation, motion, time, method, design experiments.

Plant biomass is a commonly known source of pharmaceutically valuable compounds. The study aimed to investigate the influence wave-type agitation conditions on proliferation Ringera graeca hairy roots in disposable bioreactor. Transgenic morphology, fresh increases, and yield extracellularly secreted bioactive compounds have been recognized.Кеуwordsdisposable (single-use) bioreactor, wave-assisted agitation, transgenic (hairy) roots, aeration, bioprocess intensification

Electrospinning is a process that has attracted significant interest in recent years. It provides the opportunity to produce nanofibers mimic extracellular matrix. As result, it possible use nonwovens as scaffolds characterized by high cellular adhesion. This work focused on synthesis of poly(glycerol itaconate) (PGItc) and preparation based PGItc gels polylactide. were synthesized reaction between itaconic anhydride glycerol. The mixture PLA allowed us obtain material with different...