Atrazine, is one of major concern pesticides contaminating agricultural areas and ground water. Its microbial biodegradation seems to be the most efficient in terms economic environmental benefits. In present work cometabolic atrazine by fungus Metarhizum robertsii IM 6519 during 10-day batch cultures was characterized. The herbicide transformed several hydroxy-, dechlorinated or dealkylated metabolites with involvement cytochrome P450 monooxygenases. obtained metabolomics data revealed that...

To screen environmental Bacillus strains for detection of genes encoding the enzymes involved in biosurfactant synthesis and to evaluate their products e.g. surfactin, iturin fengycin.The taxonomic identification isolated from environment was performed by Microgene ID panel GEN III Biolog system. The polymerase chain reaction (PCR) strategy screening set up. Liquid chromatography-mass spectrometry (LC-MS/MS) method used lipopeptides (LPs). All studied exhibited presence srfAA gene produced...

Abstract The influence of 50 and 100 μ m Ni on membrane integrity, hydrogen peroxide content, lipid peroxidation as well total fatty acid composition was studied in shoots roots wheat seedlings. In both organs, treatment with led to significant increase electrolyte leakage reflecting the metal‐induced enhancement permeability. shoots, it accompanied by peroxidation. Accumulation peroxide, more pronounced also found Ni‐treated wheat. Exposure seedlings altered composition, leading decrease...

Urinary tract infections are a common disease in humans. Therefore, new methods needed to destroy biofilms that formed by uropathogens. Iturin A lipopeptides (LPs) C14 and C15 potent biosurfactants synthetized the Bacillus subtilis I'1a strain. The biological activity of extracted LPs was confirmed examining extracts from cultures against uropathogenic bacteria had been isolated on urinary catheters. Compared with DSM 3257, which produce surfactin at relatively low level, extract obtained...

The study reports the response to herbicide of 2,4-dichlorophenoxyacetic acid (2,4-D)–degrading fungal strain Umbelopsis isabellina. A comparative analysis covered 41 free amino acids as well 140 lipid species fatty acids, phospholipids, acylglycerols, sphingolipids, and sterols. 2,4-D presence led a decrease in catalase activity, associated with higher amount thiobarbituric acid-reactive substances (TBARS). Damage cells treated resulted increased membrane permeability decreased fluidity....

Due to low efficacy of classic antimicrobial drugs, finding new active preparations attracts much attention. In this study an innovative, cost-effective and environmentally friendly method was applied produce silver nanoparticles (AgNPs) using filamentous fungi Metarhizium robertsii biomass waste. It shown that these NPs possess prominent antifungal effects against C. albicans, glabrata parapsilosis reference strains. Further detailed studies were performed on albicans ATCC 90028. AgNPs kill...

Candida albicans is an opportunistic fungal pathogen of humans. Treatment C. infections relies on azoles, which target the lanosterol 14α-demethylase (Erg11p) encoded by ERG11 gene. Our results show that targeted gene disruption can result in resistance to ergosterol-dependent drugs (azoles and amphotericin B), auxotrophy aerobically viable erg11Δ/Δ cells. Abnormal sterol deposition lack ergosterol strain leads reduced plasma membrane (PM) fluidity, as well dysfunction vacuolar mitochondrial...

Silver nanoparticles (AgNPs) are used in a variety of different fields due to their excellent antimicrobial potential. Despite clear advantages, concerns about toxicity have arisen, also concerning biogenic nanoparticles. Simultaneously, global healthcare is facing problem spreading resistance towards existing antibiotics. Using combined therapies involving AgNPs and antibiotics seems be promising solution the above problems. The aim this study was evaluate enhancement effectiveness AgNPs,...

To investigate the response of tributyltin-degrading fungal strain Cunninghamella elegans to organotin, a comparative lipidomics strategy was employed using an LC/MS-MS technique. A total 49 lipid species were identified. Individual phospholipids then quantified multiple reaction monitoring method. Tributyltin (TBT) caused decline in amounts many molecular phosphatidylethanolamine or phosphatidylserine and increase levels phosphatidic acid, phosphatidylinositol phosphatidylcholine. In...

The chemical 2,4-dichlorophenoxyacetic acid (2,4-D) is used in agriculture as a herbicide. Its intensive use has an adverse effect on the environment. This study involved examining degradation of 2,4-D compound by filamentous fungus Umbelopsis isabellina. After 5 days incubation, 98% herbicide (added at 25 mg L-1) was found to be removed. elimination U. isabellina connected with formation 2,4-dichlorophenol (2,4-DCP), which resulted 60% decrease sample toxicity toward Artemia franciscana...

Adherence of the fungus, Candida albicans, to biotic (e.g. human tissues) and abiotic catheters) surfaces can lead emergence opportunistic infections in humans. The process adhesion further biofilm development depends, part, on cell surface hydrophobicity (CSH). In this study, we compared resistance C. albicans strains with different CSH most commonly prescribed antifungal drug, fluconazole, newly described synergistic combination, fluconazole gentamicin. hydrophobic strain was more...

While there has been intensive research on the influence of microplastics (MPs) aquatic organisms and humans, their effect microorganisms is relatively little-known. The present study describes response Trichoderma harzianum strain to low-density polyethylene (LDPE) microparticles. MPs, either separately or with metolachlor (MET), were added cultures. Initially, MP was not found have a negative fungal growth MET degradation. After 72 h cultivation, content biomass in samples MPs almost three...

In this work we compared the effect of five heavy metals: Zn, Pb, Cd, Ni and Cu on phospholipid composition ubiquitous soil fungus Paecilomyces marquandii, originating from a strongly metal polluted area characterized by high tolerance to these elements. caused an increase in phosphatidylcholine (PC). Only Pb decreased PC content, which was accompanied significant rise phosphatidic acids (PA) level, probably due activation phospholipase D hydrolyzes PA. This could result membrane fluidity...

The opportunistic pathogen Candida albicans is responsible for life-threating infections in immunocompromised individuals. Azoles and polyenes are two of the most commonly used antifungals target ergosterol biosynthesis pathway or itself. A limited number clinically employed correspond to development resistance mechanisms. One mechanism observed clinical isolates azole-resistant C. introduction point mutations ERG11 gene, which encodes a key enzyme (lanosterol 14-α-demethylase) on pathway....

Opportunistic pathogen Candida albicans causes systemic infections named candidiasis. Due to the increasing number of multi-drug resistant clinical isolates sp., currently employed antifungals (e.g., azoles) are insufficient for combating fungal infection. One resistance mechanisms toward azoles is increased expression plasma membrane (PM) transporters Cdr1p), and such an effect was observed in C. isolates. At same time, it has been proven that a decrease PMs sphingolipids (SLs) content...

Abstract Textile industry effluents and landfill leachate contain chemicals such as dyes, heavy metals aromatic amines characterized by their mutagenicity, cytotoxicity carcinogenicity. The aim of the present study was investigation ascomycete fungus N. pironii isolated from urban postindustrial textile green space for its ability to grow retain metabolic activity in presence dye waste. Research focused mainly on amines, which had been detected via HPLC–MS/MS analysis. Presence all tested...