A laccase-producing ascomycete fungus was isolated from soil collected around the premises of a textile dye factory and identified as Nectriella pironii. Efficient laccase production achieved via synergistic action 1 mM copper sulfate ferulic acid. Extracts rapeseed oil cake, grass hay, leaf litter in pocket urban park were used for enzyme production. The highest activity (3,330 U/L) observed culture grown on extract. This is first report biosynthesis by N. also study utilization naturally...

Metarhizium fungi, essential for ecosystem function and commonly utilised in pest control, often occupy ecological niches contaminated by toxic compounds of both anthropogenic microbiological origin. The present study reveals the potential anisopliae biodegradation Fusarium mycotoxin zearalenone (ZEN), a common contaminant crops that poses significant threat to human animal health due its oestrogenic toxicity. A key aspect pathway described is degradation ZEN cleaving lactone bond, which...

Chloroxylenol (PCMX) is applied as a preservative and disinfectant in personal care products, currently recommended for use to inactivate the SARS-CoV-2 virus. Its intensive application leads release of PCMX into environment, which can have harmful impact on aquatic soil biotas. The aim this study was assess mechanism chloroxylenol biodegradation by fungal strains Cunninghamella elegans IM 1785/21GP Trametes versicolor 373, investigate ecotoxicity emerging by-products. residues formed...

To identify the enzymes potentially useful for decolorization of azo dyes, secretome ascomycetous fungus Myrothecium roridum IM6482 was studied by using a bottom-up proteomic approach. Among identified proteins, most promising dye removal laccase, which decolorized respectively, 66, 91, 79, and 80% Acid Blue 113 (AB 113), Red 27 (AR 27), Direct 14 (DB 14), Orange 7 (AO 7). The degradation dyes enhanced at wide range pH from 4 to 8. addition redox mediators allowed eliminating AB in...

Abstract Textile industry effluents and landfill leachate contain chemicals such as dyes, heavy metals aromatic amines characterized by their mutagenicity, cytotoxicity carcinogenicity. The aim of the present study was investigation ascomycete fungus N. pironii isolated from urban postindustrial textile green space for its ability to grow retain metabolic activity in presence dye waste. Research focused mainly on amines, which had been detected via HPLC–MS/MS analysis. Presence all tested...

Background: Autoimmune thyroid diseases are multifactorial with a genetic susceptibility and environmental factors. A potential role of the protein tyrosine phosphatase non-receptor type 22 (PTPN22) gene, interferon-induced helicase domain 1 (IFIH1) thyroid-stimulating hormone receptor (TSHR) gene polymorphisms on autoimmune (AITDs) in adults has been established unequivocally, but there is still lack research articles including group children.Objective hypotheses: To estimate association...

In this study, the extracts of lignocellulosic materials such as sawdust, hay, and rapeseed press cake were used medium for production laccase non-ligninolytic fungus Myrothecium roridum. Media prepared from hay extract contributed to highest quantity enzyme produced (ranging 465 728 U/L after 24 h cultivation). It could have resulted high content reducing sugars phenolic compounds gallic or ferulic acid identified in media by liquid chromatography coupled with tandem mass spectrometry. The...

The aim of this study was to overproduce, identify and apply novel laccase-like multicopper oxidases (LMCOs) from Myrothecium roridum in a dye removal process. LMCOs' production enhanced by modifying the medium adding copper ions. After purification, two proteins, LMCO1 LMCO2, with molecular masses 46.7 66.3 kDa were discovered. Peptide analysis mass spectrometry revealed that they belong cupredoxin superfamily. Characteristic peptide sequences obtained for MCOs bilirubin oxidases. Crude...

Abstract Multicopper oxidases (MCOs) are a large family of blue copper proteins which contain from one to six atoms per molecule. Their catalytic centre consists three domains involve type I Cu, II Cu and pair III Cu’s. They include laccases, ferroxidases, ascorbate oxidase, bilirubin laccase-like multicopper oxidases. MCOs capable one-electron oxidizing aromatic as well non-aromatic compounds with concomitant four-electron reduction molecular oxygen water. These properties make them...

The ascomycete fungus Nectriella pironii, previously isolated from soil continuously contaminated by dye industry waste, was used for the biodegradation of phenanthrene (PHE), benz[a]anthracene (B[a]A), and benz[a]pyrene (B[a]P). degradation polycyclic aromatic hydrocarbons (PAHs) N. pironii accelerated in presence landfill leachate (LL) collected area isolation. rate cometabolic elimination PHE B[a]P LL was, respectively, 75% 94% higher than its absence. LC-MS/MS analysis revealed that PAHs...

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