A5103057842- Plant Gene Expression Analysis
- Horticultural and Viticultural Research
- Plant biochemistry and biosynthesis
- Light effects on plants
- Plant Molecular Biology Research
- Biochemical and biochemical processes
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
Pontificia Universidad Católica de Chile
2015-2023
Because of the vast range functions that phenylpropanoids possess, their synthesis requires precise spatiotemporal coordination throughout plant development and in response to environment. The accumulation these secondary metabolites is transcriptionally controlled by positive negative regulators from MYB basic helix-loop-helix protein families. We characterized four grapevine (Vitis vinifera) R2R3-MYB proteins C2 repressor motif clade, all which harbor ethylene factor-associated amphiphilic...
Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related UV-B responses have not been molecularly characterized. We cloned and studied the role of RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), HY5 HOMOLOGUE (HYH) from V. vinifera. performed gene functional characterizations, generated co-expression networks, tested them in different environmental conditions. These genes complemented Arabidopsis uvr8 hy5 mutants...
Summary Grapevine organs accumulate anthocyanins in a cultivar‐specific and environmentally induced manner. The MYBA 1 ‐ A2 genes within the berry color locus chromosome 2 represent major genetic determinants of fruit color. simultaneous occurrence transposon insertions point mutations these is responsible for most white‐skinned phenotypes; however, red pigmentation found vegetative suggests presence additional regulators. This work describes genomic region 14 containing three closely...
Variegation is a rare type of mosaicism not fully studied in plants, especially fruits. We examined red and white sections grape (Vitis vinifera cv. 'Béquignol') variegated berries found that accumulation products from branches the phenylpropanoid isoprenoid pathways showed an opposite tendency. Light-responsive flavonol monoterpene levels increased anthocyanin-depleted areas correlation with increasing MYB24 expression. Cistrome analysis suggested binds to promoters 22 terpene synthase...
Abstract We explored the effects of ultraviolet B radiation (UV-B) on developmental dynamics microRNAs and phased small-interfering-RNA (phasi-RNAs)-producing loci by sequencing small RNAs in vegetative reproductive organs grapevine (Vitis vinifera L.). In particular, we tested different UV-B conditions vitro-grown plantlets (high-fluence exposition) berries from field-grown (radiation filtering) greenhouse-grown (low- high-fluence expositions) adult plants throughout fruit development...
Flavonols constitute a group of flavonoids with important photoprotective roles in plants. In addition, flavonol content and composition greatly influences fruit quality. We previously demonstrated that the grapevine R2R3-MYB transcription factor (TF) VviMYBF1 promotes accumulation by inducing expression synthase (VviFLS1/VviFLS4), key step initial pathway. Despite this, gene networks underlying modification including both structural regulatory genes remain poorly understood. order to...
The woody nature of grapevine ( Vitis vinifera L.) has hindered the development efficient gene editing strategies to improve this species. lack highly transfer techniques, which, furthermore, are applied in multicellular explants such as somatic embryos, additional technical handicaps vine. inclusion geminivirus-based replicons regular T-DNA vectors can enhance expression clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) elements, thus...
Abstract The presence of naturally-occurring color mutants in plants has permitted the identification many regulatory genes implicated synthesis discrete metabolic compounds, mostly anthocyanins and carotenoids. Conversely, transcription factors that coordinate more than one specialized pathway seem challenging to screen from a forward genetics’ perspective. We explored relationship between different branches phenylpropanoid isoprenoid pathways while examining an infrequent berry skin...
Abstract UV-B regulation of anthocyanin biosynthesis in vegetative and grapevine berry tissues has been extensively described. However, its relation with UV-B-regulated microRNAs (miRNAs) not addressed before this species. We explored by deep sequencing small RNA libraries the developmental dynamics effects on miRNAs associated phased interfering (phasi-RNAs)-producing loci abundances vitro -grown plantlets, field-grown skins cv. Cabernet Sauvignon, low- high fluence treatments...