A5002674451- Intraocular Surgery and Lenses
- Corneal surgery and disorders
- Atomic and Molecular Physics
- Retinal and Macular Surgery
- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Ophthalmology and Visual Impairment Studies
- Particle Accelerators and Free-Electron Lasers
- Nuclear physics research studies
- Plant Pathogens and Fungal Diseases
- Horticultural and Viticultural Research
- Cold Atom Physics and Bose-Einstein Condensates
- Plant Gene Expression Analysis
- Retinal Diseases and Treatments
- Scientific Measurement and Uncertainty Evaluation
- Advanced Frequency and Time Standards
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Fermentation and Sensory Analysis
- Laser-Plasma Interactions and Diagnostics
- Advanced X-ray Imaging Techniques
- Crystallography and Radiation Phenomena
- Circular RNAs in diseases
- Biochemical and biochemical processes
- Ocular and Laser Science Research
Apple (Germany)
2019-2024
Heidelberg University
2012-2024
University Hospital Heidelberg
2009-2024
Dienstleistungszentrum Ländlicher Raum
2014-2022
Dienstleistungszentrum Ländlicher Raum Rheinpfalz
2014-2022
Augenklinik Heidelberg
2017-2022
Creative Commons
2021
Klaus Tschira Foundation
2021
Davao Doctors Hospital
2021
Minnesota Lions Eye Bank
2020
Using a new bunched-beam technique in the GSI heavy-ion experimental storage ring (ESR), we performed precision laser spectroscopy on relativistic heavy ions hitherto inaccessible infrared optical region. We determined wavelength of $M1$ transition between $F\phantom{\rule{0ex}{0ex}}=\phantom{\rule{0ex}{0ex}}1$ $(\ensuremath{\tau}\ensuremath{\approx}50\mathrm{ms})$ and $F\phantom{\rule{0ex}{0ex}}=\phantom{\rule{0ex}{0ex}}0$ hyperfine states $1s$ ground state hydrogenlike...
The stilbenoid pathway is responsible for the production of resveratrol in grapevine (Vitis vinifera L.). A few transcription factors (TFs) have been identified as regulators this but extent control has not deeply studied. Here we show how DNA affinity purification sequencing (DAP-Seq) allows genome-wide TF-binding site interrogation grape. We obtained 5190 and 4443 binding events assigned to 4041 3626 genes MYB14 MYB15, respectively (approximately 40% peaks located within −10 kb start...
Grapevine ( Vitis vinifera ssp. ) is one of the most important fruit species; however, it highly susceptible to various pathogens, which can cause severe crop losses in viticulture. It has been shown that several WRKY class transcription factors (TFs) are part signal transduction cascade, leads activation plant defense reactions against pathogens. In present investigation, a full‐length cDNA was isolated from V. leaf tissue encoding predicted protein, designated VvWRKY33, shows...
The purpose of this study was to describe a novel device that may serve as an alternative Descemet membrane endothelial keratoplasty (DMEK) for the treatment chronic corneal edema.The EndoArt (EyeYon Medical, Israel) is flexible, 50-μm thin artificial layer matches cornea's posterior curvature and functions fluid barrier at stroma, replacing diseased endothelium. Similar DMEK approach, it implanted into anterior chamber, carefully positioned on secured using air-gas mixture. Two patients...
Stilbene synthase is a key enzyme for the production of phytoalexin resveratrol. Some clones Vitis sylvestris, wild European grapevine species which almost extinct, have been shown to accumulate more resveratrol in response different forms stress. In current study, we asked whether induction stilbene transcripts Hoe29, one V. sylvestris with elevated inducibility, might result from transcription factor MYB14. The MYB14 promoter Hoe29 and Ke83 (a second stilbene-inducible genotype) harboured...
With recent advances in technology and introduction of new intraocular lens (IOL) models, surgeons today have the opportunity to choose from various optical designs, which can influence postoperative quality vision. In our laboratory study, we compared three different IOLs that use identical platform are produced by same manufacturer. The study included two diffractive multifocal IOLs, a bifocal trifocal one, as well monofocal IOL. Three IOL models: CT ASPHINA 409 M, AT LISA 809 Tri 839MP...
Foldable hydrophobic acrylic intraocular lenses (IOLs) are prone to develop a long-term postoperative material change called glistenings. The aim of this study was investigate the changes in predisposition for glistening formation one type IOL from its introduction present day. In laboratory setup, an vitro model used induce glistenings IOLs manufactured by company (Alcon, Fort Worth, TX, USA) different years: 23 1990s-manufacture three-piece (MA30BA/MA60AC) that were explanted 1996 and...
While therapeutic success of the limbal tissue or cell transplantation to treat severe cases stem (LSC) deficiency (LSCD) strongly depends on percentage LSCs within transplanted cells, prospective LSC enrichment has been hampered by intranuclear localization previously reported marker p63. The recent identification ATP-binding cassette transporter ABCB5 as a plasma membrane-spanning that are capable restoring cornea and development an antibody directed against extracellular loop molecule...
Purpose Ray propagation visualization and optical performance analysis of four different intraocular lenses (IOLs) Methods In this laboratory study, IOLs with designs were assessed: a monofocal AcrySof IQ SN60WF [Alcon], diffractive-refractive bifocal Restor SN6AD1 diffractive trifocal PanOptix TFNT00 extended-depth-of-focus (EDOF) Symfony ZXR00 [Johnson&Johnson]. An experimental set-up water bath containing 0.01% fluorescein solution monochromatic green laser light (532 nm) was used to...
Abstract Background The formation of fluid-filled microvacuoles, termed glistenings, is a common complication intraocular lenses (IOLs) made from hydrophobic acrylate. Using our well-established in-vitro laboratory method, we evaluated new IOL material’s resistance to glistening formation. Methods An stress test for induction was performed on 20 samples acrylic IOLs: ten the Eyecryl ASHFY600 (Biotech Vision Care, Ahmedabad, India) compared with AcrySof IQ SN60WF (Alcon, Fort Worth, USA)....
Ingrowth of newly formed blood and lymph vessels (angiogenesis) from the limbus region into cornea can be treated successfully by subconjunctival application antiangiogenic agents. Currently, there are several angiogenesis inhibitors various manufacturers available, such as vascular endothelial growth factor (VEGF) antibodies. The aim study was to investigate potential cytotoxic effects two anti-VEGF agents, ranibizumab (Lucentis®) bevacizumab (Avastin®) on human corneal endothelium.Human...
Analysis of explanted intraocular lenses (IOLs) from pseudophakic eyes with supplementary sulcus-supported IOLs.In this laboratory investigation, ten and capsular bag IOLs were analyzed. All received between January 2012 March 2018. Explants examined morphologically histological electron microscopic techniques patients' medical history was evaluated. Additionally, we used a technique new to field: Transmission Electron Microscopy diffraction pattern analysis performed investigate the...
Abstract Background Glistenings describe small, refractile microvacuoles that may arise within the intraocular lens (IOL) material and reduce patients’ quality of vision. Lenses composed hydrophobic acrylic are particularly affected by glistening formation. In this study, we compared tendency formation in six different types lenses (IOLs). Methods We used a well-established accelerated laboratory method to develop glistenings following IOLs: Vivinex XY1 (Hoya), AcrySof SN60WF (Alcon), Tecnis...
Purpose: To compare 1 new intraocular lens (IOL) injector system against 3 standard systems in porcine eyes. Setting: David J Apple Center for Vision Research, Department of Ophthalmology, University Hospital Heidelberg, Germany. Design: In vitro laboratory study. Methods: 70 eyes, +20.0 diopter IOLs were implanted with the following systems: multiSert, UltraSert, iTec, and RayOne, that is, S1.8 (incision size: 1.8 mm), S2.0 (2.0 S2.2P (2.2 mm, push mode), S2.2S screw U2.2 iT2.2 R2.0 mm)....
Sensitive differential proteomic analysis is challenging and often limited by distinct labeling or tagging strategies. In this study, we have examined the sensitivity, linearity, photophysical properties of novel protein DY-maleimide dyes (DY-505-MAL, DY-555-MAL DY-635-MAL). All MS compatible exhibited excellent emission spectra, high when applied to standard 1-DE analysis. Correspondingly, 2-DE DY-635-MAL DY-505-MAL maximal-labeled human keratinocyte proteins displayed remarkably...
During phacoemulsification, the corneal endothelium is protected by an ophthalmic viscosurgical device (OVD). In this in vitro study, we assessed six different OVDs for their effectiveness protecting endothelium.Phacoemulsification was performed cadaver eyes of young pigs. Five syringe units were tested (Healon EndoCoat, Viscoat, Methylvisc, Healon, Healon GV, ProVisc). After surgery, area coated with OVD determined relation to total endothelial surface. Additionally, cell count obtained. As...
Abstract During intraocular lens (IOL) implantation it is not uncommon for the injector’s nozzle-tip to get damaged. However, damage has been systematically described or evaluated using an objective scale. In this study we developed our own system—the Heidelberg Score IOL Injector Damage (“HeiScore”), which was used grade 60 injectors from four generations of injector models (Monarch III D, AcrySert C, UltraSert, AutonoMe) made by same manufacturer. (Alcon Laboratories Inc.) HeiScore six...
Purpose: The eye can see pulsed near-infrared (IR) radiation with the color corresponding to half of wavelength used. Until recently, technology required for measuring IR vision was confined optical laboratories and not studied clinically. current investigation sought determine values thresholds in a healthy population. Methods: IR-light threshold measured 45 participants, aged from 21 70 years. Ten patients retinal pathology were included comparison. Ocular media clarity assessed straylight...
Silicone oil endotamponades provide a reservoir for drugs in the eye. Following vitrectomy surgery to treat retinal detachments, extensive diabetic retinopathy or endophthalmitis, they can be used as long-term lipophilic depots. This study aimed investigate physicochemical properties of intravitreally applied different lipophilicity, namely vancomycin, ceftazidime and voriconazole. For this purpose, an vitro model silicone-oil-filled eye compared porcine vitreous bodies (PVBs) was used. In...
Smaller corneal incisions in cataract surgery are linked with a better visual outcome and less frequent postoperative endophthalmitis. The insertion of intraocular lens (IOL) injector systems into the anterior chamber eye to implant an IOL is associated incision enlargement (IE) impeding these positive effects. aim this study was compare manufacturers' recommended sizes (IS) 13 different regard intraoperative IE IS. In total, 499 ex vivo porcine eyes were analyzed. preoperative ISs depended...