A5007325258- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Toxin Mechanisms and Immunotoxins
- Microbial Metabolic Engineering and Bioproduction
- Insect and Arachnid Ecology and Behavior
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Biochemical and Structural Characterization
- Plant Pathogens and Resistance
- Neurobiology and Insect Physiology Research
- Postharvest Quality and Shelf Life Management
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Insect and Pesticide Research
- Microbial Natural Products and Biosynthesis
- Engineering and Information Technology
- Photosynthetic Processes and Mechanisms
Instituto de Biología Molecular y Celular de Plantas
2008-2023
Consejo Superior de Investigaciones Científicas
2008-2023
Universitat Politècnica de València
2012-2023
Unidades Centrales Científico-Técnicas
2012
John Innes Centre
2009
Wageningen University & Research
2002-2007
Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic parts in a standardized way. Here we present GoldenBraid (GB), system based on type IIS restriction enzymes that allows indefinite growth reusable gene modules made pieces. The GB consists set four destination plasmids (pDGBs) designed incorporate multipartite assemblies standard combine them binarily build increasingly complex multigene constructs....
The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety applications in plants, ranging from gene editing to construction transcriptional circuits, many which depend on technical ability compose transfer complex synthetic instructions into plant cell. principles standardization modularity applied DNA cloning are impacting genetic engineering, by increasing multigene assembly efficiency fostering exchange...
Modular DNA assembly simplifies multigene engineering in Plant Synthetic Biology. Furthermore, the recent adoption of a common syntax to facilitate exchange plant parts (phytobricks) is promising strategy speed up genetic engineering. Following this lead, here, we present platform for biodesign that incorporates functional descriptions phytobricks obtained under pre-defined experimental conditions, and systematically registers resulting information as metadata documentation. To handling...
Abstract Virus-induced gene silencing (VIGS) is a powerful tool for reverse genetics in tomato (Solanum lycopersicum). However, the irregular distribution of effects VIGS hampers identification and quantification nonvisual phenotypes. To overcome this limitation, visually traceable system was developed fruit, comprising two elements: (1) transgenic line (Del/Ros1) expressing Antirrhinum majus Delila Rosea1 transcription factors under control fruit-specific E8 promoter, showing...
Volatile organic compounds (VOCs) are major determinants of fruit flavor, a primary objective in tomato breeding. A recombinant inbred line (RIL) population consisting 169 lines derived from cross between Solanum lycopersicum and red-fruited wild species pimpinellifolium accession (SP) was characterized for VOCs three different seasons. Correlation hierarchical cluster analyses were performed on the 52 identified, providing tool putative assignation individual to metabolic pathways....
The current CoVid-19 crisis is revealing the strengths and weaknesses of world’s capacity to respond a global health crisis. A critical weakness has resulted from excessive centralization biomanufacturing capacities, matter great concern, if not source nationalistic tensions. On positive side, scientific data information have been shared at an unprecedented speed fuelled by preprint phenomena, this considerably strengthened our ability develop new technology-based solutions. In work, we...
Summary CRISPR/Cas has revolutionized genome engineering in plants. However, the use of anti‐CRISPR proteins as tools to prevent CRISPR/Cas‐mediated gene editing and activation plants not been explored yet. This study describes characterization two proteins, AcrIIA4 AcrVA1, Nicotiana benthamiana . Our results demonstrate that prevents site‐directed mutagenesis leaves when transiently co‐expressed with CRISPR/Cas9. In a similar way, AcrVA1 is able CRISPR/Cas12a‐mediated editing. Moreover,...
Abstract Antivenoms developed from the plasma of hyperimmunized animals are only effective treatment available against snakebite envenomation but shortage supply contributes to high morbidity and mortality toll this tropical disease. We describe a synthetic biology approach affordable cost‐effective antivenom production based on plant‐made recombinant polyclonal antibodies (termed pluribodies). The strategy takes advantage virus superinfection exclusion induce formation somatic expression...
Plant-based bioproduction of insect sex pheromones has been proposed as an innovative strategy to increase the sustainability pest control in agriculture. Here, we describe engineering transgenic plants producing (Z)-11-hexadecenol (Z11-16OH) and (Z)-11-hexadecenyl acetate (Z11-16OAc), two main volatile components many Lepidoptera pheromone blends. We assembled multigene DNA constructs encoding biosynthetic pathway stably transformed them into Nicotiana benthamiana plants. The contained...
CRISPR/Cas ability to target several loci simultaneously (multiplexing) is a game-changer in plant breeding. Multiplexing not only accelerates trait pyramiding but also can unveil traits hidden by functional redundancy. Furthermore, multiplexing enhances dCas-based programmable gene expression and enables cascade-like regulation. However, the design assembly of multiplex constructs comprising tandemly arrayed guide RNAs (gRNAs) requires scarless cloning still troublesome due presence...
Summary Expression profiling by cDNA‐AFLP is commonly used to display the transcriptome of a specific tissue, treatment or developmental stage. In this paper, has been study transcripts expressed in segregating populations from Arabidopsis thaliana and potato ( Solanum tuberosum ). The genetic differences between offspring genotypes are thus visualized as polymorphisms transcriptome. We show that polymorphic can be markers allow construction linkage map. resulting map shows that, contrast...
SQUAMOSA PROMOTER BINDING-LIKE (SPL) proteins constitute a large family of transcription factors known to play key roles in growth and developmental processes, including juvenile-to-adult vegetative-to-reproductive phase transitions. This makes SPLs interesting targets for precision breeding plants the Nicotiana genus used as e.g. recombinant biofactories. We report identification 49 SPL genes tabacum cv. K326 43 benthamiana LAB strain, which were classified into eight phylogenetic groups...
A combination of cDNA-amplified fragment length polymorphism (AFLP) and bulked segregant analysis (BSA) was used to identify genes co-segregating with earliness tuberization in a diploid potato population. This approach identified 37 transcript-derived fragments polymorphic segregation pattern between early late tuberizing bulks. Most the transcripts mapped chromosomes 5 (19 markers) 12 (eight paternal map. Quantitative trait locus (QTL) mapping time also QTLs on these two chromosomes....
The squalene synthase (SQS) gene encodes a key regulatory enzyme, farnesyl-diphosphate farnesyltransferase (EC 2.5.1.21), in sterol biosynthesis. SQS1 was isolated from subgenomic library of the industrially important yeast Yarrowia lipolytica, using PCR-generated probes. Probes were based on conserved regions homologues different organisms. complete nucleotide sequence coding region and corresponding amino acid determined. sequences showed extensive homologies with genes enzymes number...
Considering cells as biofactories, we aimed to optimize its internal processes by using the same engineering principles that large industries are implementing nowadays: lean manufacturing. We have applied reverse computational methods transcriptomic, metabolomic and phenomic data obtained from a collection of tomato recombinant inbreed lines formulate kinetic constraint-based model efficiently describes cellular metabolism expression minimal core genes. Based on predicted metabolic profiles,...
The production of neutralizing immunoglobulin A (IgA) in edible fruits as a means oral passive immunization is promising strategy for the inexpensive treatment mucosal diseases. This approach based on assumption that status remains unaltered immunoglobulin-expressing fruit, and therefore extensive purification not required delivery. However, unintended effects associated with IgA expression such toxic secondary metabolites protein allergens cannot be dismissed priori need to investigated....
Summary Higher dietary intakes of flavonoids may have a beneficial role in cardiovascular disease prevention. Additionally, supplementation branched‐chain amino acids (BCAAs) vegan diets can reduce risks associated to their deficiency, particularly older adults, which cause loss skeletal muscle strength and mass. Most plant‐derived foods contain only small amounts BCAAs, those plants with high levels are not eaten broadly. Here we describe the generation metabolically engineered cisgenic...
ABSTRACT The current CoVid-19 crisis is revealing the strengths and weaknesses of world’s capacity to respond a global health crisis. A critical weakness has resulted from excessive centralization biomanufacturing capacities, matter great concern, if not source nationalistic tensions. On positive side, scientific data information have been shared at an unprecedented speed fuelled by preprint phenomena, this considerably strengthened our ability develop new technology-based solutions. In work...