A5072545959- Chromatin Remodeling and Cancer
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Phagocytosis and Immune Regulation
- Cellular transport and secretion
- Plant tissue culture and regeneration
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Mechanisms of cancer metastasis
- RNA regulation and disease
- Genetics, Aging, and Longevity in Model Organisms
- Plant Molecular Biology Research
Instituto de Biología Molecular y Celular de Plantas
2019-2025
Consejo Superior de Investigaciones Científicas
2019-2025
Universitat Politècnica de València
2019-2025
The current CoVid-19 crisis is revealing the strengths and weaknesses of world’s capacity to respond a global health crisis. A critical weakness has resulted from excessive centralization biomanufacturing capacities, matter great concern, if not source nationalistic tensions. On positive side, scientific data information have been shared at an unprecedented speed fuelled by preprint phenomena, this considerably strengthened our ability develop new technology-based solutions. In work, we...
Abstract Background CRISPR-based programmable transcriptional activators (PTAs) are used in plants for rewiring gene networks. Better tuning of their activity a time and dose-dependent manner should allow precise control expression. Here, we report the optimization Copper Inducible system called CI-switch conditional activation Nicotiana benthamiana . In presence copper, copper-responsive factor CUP2 undergoes conformational change binds DNA motif named copper-binding site (CBS). Results...
Abstract Eukaryotes have evolved multiple ATP-dependent chromatin remodelers to shape the nucleosome landscape. We recently uncovered an evolutionarily conserved SWItch/Sucrose Non-Fermentable (SWI/SNF) remodeler complex in plants reminiscent of mammalian BAF subclass, which specifically incorporates MINUSCULE (MINU) catalytic subunits and TRIPLE PHD FINGERS (TPF) signature subunits. Here we report experimental evidence that establishes functional relevance TPF proteins for activity. Our...
Over millions of years, eukaryotes evolved from unicellular to multicellular organisms with increasingly complex genomes and sophisticated gene expression networks. Consequently, chromatin regulators support this increased complexity. The ATP-dependent remodelers the SWI/SNF family are multiprotein complexes that modulate nucleosome positioning appear under different configurations, which perform distinct functions. While composition, architecture, activity these subclasses well understood...
The SWItch/Sucrose Non-Fermenting (SWI/SNF) complexes are evolutionarily conserved, ATP-dependent chromatin remodelers crucial for multiple nuclear functions in eukaryotes. Recently, plant BCL-DOMAIN HOMOLOG (BDH) proteins were identified as shared subunits of all SWI/SNF complexes, significantly impacting accessibility and various developmental processes Arabidopsis. In this study, we performed a comprehensive characterization bdh mutants, revealing the role BDH hypocotyl cell elongation....
Abstract The SWItch/Sucrose Non-Fermenting (SWI/SNF) complexes are evolutionarily conserved, ATP-dependent chromatin remodelers crucial for multiple nuclear functions in eukaryotes. Recently, plant BCL-Domain Homolog (BDH) proteins were identified as shared subunits of all SWI/SNF complexes, significantly impacting accessibility and various developmental processes Arabidopsis. In this study, we performed a comprehensive characterization bdh mutants, revealing previously overlooked impact on...
ABSTRACT The current CoVid-19 crisis is revealing the strengths and weaknesses of world’s capacity to respond a global health crisis. A critical weakness has resulted from excessive centralization biomanufacturing capacities, matter great concern, if not source nationalistic tensions. On positive side, scientific data information have been shared at an unprecedented speed fuelled by preprint phenomena, this considerably strengthened our ability develop new technology-based solutions. In work...
Abstract CRISPR-based programmable transcriptional activators (PTAs) are used in plants for rewiring gene networks. Better tuning of their activity a time and dose-dependent manner should allow precise control expression. Here, we report the optimization Copper Inducible system called CI-switch conditional activation Nicotiana benthamiana . In presence copper, copper-responsive factor CUP2 undergoes conformational change binds DNA motif named copper-binding site (CBS). this study, tested...
ABSTRACT Eukaryotes have evolved multiple ATP-dependent chromatin remodelers to shape the nucleosome landscape. We recently uncovered an evolutionarily conserved SWItch/Sucrose Non-Fermentable (SWI/SNF) remodeler complex in plants reminiscent of mammalian BAF subclass, which specifically incorporates MINUSCULE (MINU) catalytic subunits and TRIPLE PHD FINGERS (TPF) signature subunits. Here we report experimental evidence that establishes functional relevance TPF proteins for activity. Our...