A5073529248- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
Instituto de Biología Molecular y Celular de Plantas
2020-2025
Consejo Superior de Investigaciones Científicas
2020-2025
Universitat Politècnica de València
2020-2025
Abstract The huge diversity of cultivated tomatoes is the result a long process domestication followed by intensive breeding. Breeding efforts have been focused on increasing fruit size and diversification phenotypes. formation seedless (parthenocarpic) fruits in tomato plants an interesting trait for growers, providing mechanism to overcome fertilization failure under unfavourable environmental conditions. Early anther or pollen ablation effective strategy promote parthenocarpy was proven...
CRISPR/Cas ability to target several loci simultaneously (multiplexing) is a game-changer in plant breeding. Multiplexing not only accelerates trait pyramiding but also can unveil traits hidden by functional redundancy. Furthermore, multiplexing enhances dCas-based programmable gene expression and enables cascade-like regulation. However, the design assembly of multiplex constructs comprising tandemly arrayed guide RNAs (gRNAs) requires scarless cloning still troublesome due presence...
The tapetum is a specialized layer of cells within the anther, adjacent to sporogenous tissue. During its short life, it provides nutrients, molecules and materials pollen mother microsporocytes, being essential during callose degradation wall formation. interaction between in Solanum lycopersicum (tomato) plants, despite importance for breeding purposes, poorly understood. To investigate this process, gene editing was used generate loss-of-function mutants that showed complete specific...
Argan trees (Argania spinosa) belong to a species native southwestern Morocco, playing an important role in the environment and local economy. oil extracted from kernels has unique composition properties. were introduced Tunisia, where hundreds of can be found nowadays. In this study, we examined reproductive development four sites Tunisia carried out functional characterization floral homeotic gene non-model species. Despite importance development, nothing is known about genetic network...
ABSTRACT CRISPR/Cas ability to target several loci simultaneously (multiplexing) is a game-changer in plant breeding. Multiplexing not only accelerates trait pyramiding but also can unveil traits hidden by functional redundancy polyploid crops. Furthermore, multiplexing enhances dCas-based programmable gene expression and enables cascade-like regulation. However, multiplex constructs comprising tandemly arrayed gRNAs are difficult assemble, this hampering more widespread use. Here we present...
ABSTRACT The tapetum is a specialized layer of cells within the anther adjacent to sporogenic tissue. During its short life, it provides nutrients, molecules and materials pollen mother microsporocytes being essential during callose degradation wall formation. However, acquisition tapetal cell identity in tomato plants process still poorly understood. We report here identification characterization SlTPD1 ( Solanum lycopersicum TPD1 ), gene specifically required for development plants. Gene...