A5055959433- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Agricultural Practices and Plant Genetics
- Gamma-ray bursts and supernovae
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- SAS software applications and methods
- Plant and soil sciences
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Resistance
- Postharvest Quality and Shelf Life Management
- Plant Molecular Biology Research
- Plant and animal studies
- Plant Physiology and Cultivation Studies
- Plant Parasitism and Resistance
- Banana Cultivation and Research
- RNA and protein synthesis mechanisms
- Plant Surface Properties and Treatments
- Plant pathogens and resistance mechanisms
- Spectroscopy and Chemometric Analyses
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Astronomy and Astrophysical Research
- Insect and Pesticide Research
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"
2014-2024
Universidad de Málaga
2014-2024
Consejo Superior de Investigaciones Científicas
2012-2023
Unidades Centrales Científico-Técnicas
2012
Hospital Universitario Ramón y Cajal
2002
Rockefeller University
1977
Walter Reed Army Institute of Research
1975
Columbia University
1974
Centro de Investigaciones Biológicas Margarita Salas
1971
Pretty or Sweet The grocery-store tomato that looks beautiful but tastes like tart cardboard arises from selection processes favoring phenotypes make commercial production more reliable. Significant in process was a mutation reduced the mottled color variations of unripe green tomatoes, leaving them uniform, pale, green. Powell et al. (p. 1711 ) analyzed molecular biology mutation. uniform ripening turns out to disable transcription factor called Golden 2-like ( GLK2 ). expression increases...
In Escherichia coli there is one binding site per ribosome for lincomycin, chloramphenicol and erythromycin. These antibiotics act at closely related sites on the 50‐S subunit. There are, however, some interesting differences in properties of three sites, such as: (a) apparent possibility obstructing lincomycin without closing site, (b) asymmetry competition effects between erythromycin, (c) differential responses to ethanol (d) other (puromycin, macrolides, streptogramins A B others also...
Multiple viral infections frequently are found in single plants of cultivated and wild hosts nature, with unpredictable pathological consequences. Synergistic reactions were observed mixed tomato doubly infected the positive-sense phloem-limited single-stranded RNA (ssRNA) crinivirus Tomato chlorosis virus (ToCV) negative-sense ssRNA tospovirus spotted wilt (TSWV). Synergism a cultivar susceptible to both viruses resulted rapid death plants. A pronounced enhancement ToCV accumulation...
Breeding of tomato genotypes that limit whitefly (Bemisia tabaci) access and feeding might reduce the spread Tomato yellow leaf curl virus (TYLCV), a begomovirus (genus Begomovirus, family Geminiviridae) is causal agent disease. TYLCV restricted to phloem transmitted in persistent manner by B. tabaci. The breeding line ABL 14-8 was developed introgressing type IV glandular trichomes secretion acylsucroses from wild Solanum pimpinellifolium accession TO-937 into genetic background whitefly-...
A new method was developed for isolation of intracellular forms simian virus 40 (SV40) nucleoprotein complexes from SV40-infected CV-1 cells late in the infectious cycle. In contrast to Triton extraction method, which yields only a 60-70S complex, this procedure yielded three SV40 complexes: complex I, II, and nature virion (V). The differed physical as well biochemical properties. Complex is small portion total SV42 during infection, active synthesizing both SV40-specific DNA RNA....
Volatile organic compounds (VOCs) are major determinants of fruit flavor, a primary objective in tomato breeding. A recombinant inbred line (RIL) population consisting 169 lines derived from cross between Solanum lycopersicum and red-fruited wild species pimpinellifolium accession (SP) was characterized for VOCs three different seasons. Correlation hierarchical cluster analyses were performed on the 52 identified, providing tool putative assignation individual to metabolic pathways....
Abstract Tomato (Solanum lycopersicum) fruit ripening is accompanied by an increase in CHALCONE SYNTHASE (CHS) activity and flavonoid biosynthesis. Flavonoids accumulate the cuticle, giving its characteristic orange color that contributes to eventual red of ripe fruit. Using virus-induced gene silencing fruits, we have down-regulated expression SlCHS during compared cuticles derived from silenced nonsilenced regions. Silenced regions showed a pink due lack flavonoids incorporated cuticle....
Background The whitefly Bemisia tabaci (Genn.) causes dramatic damage to plants by transmitting yield-limiting virus diseases. Previous studies proved that the tomato breeding line ABL 14-8 was resistant B. tabaci, vector of yellow leaf curl disease (TYLCD). This resistance is based on presence type IV glandular trichomes and acylsucrose production. These deter settling probing in 14-8, which reduces primary secondary spread TYLCD. Methodology/Principal Findings Whitefly settlement...
Whiteflies damage tomatoes mostly via the viruses they transmit. Cultivated lack many of resistances their wild relatives. In order to increase protection its major pest, whitefly Bemisia tabaci and transmitted Tomato Yellow Leaf Curl Virus (TYLCV), we introgressed a trichome-based resistance trait from tomato Solanum pimpinellifolium into cultivated tomato, lycopersicum. The backcross line BC5S2 contains acylsucrose-producing type-IV trichomes, unlike tomatoes, exhibits increased, yet...
We have studied a genomic library of introgression lines from the S. pimpinellifolium accession TO-937 into genetic background "Moneymaker" cultivar in order to evaluate accession's breeding potential. Overall, no deleterious phenotypes were observed, and plants fruits phenotypically very similar those "Moneymaker", which confirms feasibility translating current results elite programs. identified chromosomal regions associated with traits that both vegetative (plant vigor, trichome density)...
Tomato yellow leaf curl disease (TYLCD) causes severe damage to tomato crops in warm regions of the world, and is associated with infections several whitefly (Bemisia tabaci)-transmitted single-stranded (ss)DNA begomoviruses (genus Begomovirus, family Geminiviridae). The most widespread begomovirus isolates TYLCD are those type strain virus species, known as Israel (TYLCV-IL). Ty-1 gene widely used commercial cultivars control TYLCV-IL damage, providing resistance by restricting viral...
Tomato chlorosis virus (ToCV) (genus Crinivirus, family Closteroviridae) is an emerging threat to tomato crops worldwide. Although symptoms on fruits are not obvious, yield losses occur through decreased fruit size and number. Control of ToCV epidemics difficult because the transmitted by several whitefly vector species its relatively wide host range facilitates establishment in local wild reservoirs. Therefore, breeding for resistance offers best control alternative. However, no sources...
Abstract Background L-ascorbic acid (AsA; vitamin C) is essential for all living plants where it functions as the main hydrosoluble antioxidant. It has diverse roles in regulation of plant cell growth and expansion, photosynthesis, hormone-regulated processes. AsA also an component human diet, being tomato fruit one sources this vitamin. To identify genes responsible content fruit, transcriptomic studies followed by clustering analysis were applied to two groups fruits with contrasting...
Increasing L-ascorbic acid (AsA, vitamin C) content in fruits is a common goal current breeding programs due to its beneficial effect on human health. Attempts increase AsA by genetic engineering have resulted variable success likely AsA's complex regulation. Here, we report the of ectopically expressing tomato D-galacturonate reductase (FaGalUR) gene from strawberry, involved biosynthesis, either under control constitutive 35S or fruit-specific polygalucturonase (PG) promoters. Although...
Tomato chlorosis virus (genus Crinivirus, family Closteroviridae) (ToCV) is rapidly emerging, causing increased damage to tomato production worldwide. The transmitted in a semipersistent manner by several whitefly (Hemiptera: Aleyrodidae) species and expanding its geographical host ranges associated with the emergence of whiteflies Bemisia tabaci complex. Control based essentially on intensive insecticide applications against insect vector but largely ineffective. No virus-resistant or...