A5020620137- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Potato Plant Research
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Microbial Metabolic Engineering and Bioproduction
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Microbial Natural Products and Biosynthesis
- Horticultural and Viticultural Research
- Bioinformatics and Genomic Networks
- Plant Surface Properties and Treatments
- Plant tissue culture and regeneration
- Insect-Plant Interactions and Control
- Antioxidant Activity and Oxidative Stress
- Biofuel production and bioconversion
- Plant and animal studies
- Advances in Cucurbitaceae Research
- Histone Deacetylase Inhibitors Research
- 14-3-3 protein interactions
- Light effects on plants
Columbia University
2025
Royal Horticultural Society
2024
Michigan State University
2012-2022
Robert W. Holley Center for Agriculture & Health
2012
United States Department of Agriculture
2005-2012
Cornell University
2001-2012
Ithaca College
2001-2008
University of Nottingham
1993-2005
Research International (United States)
2005
University of Warwick
2005
Abstract 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is one of the key regulatory enzymes involved in synthesis hormone ethylene and encoded by a multigene family containing at least eight members tomato (Lycopersicon esculentum). Increased production accompanies ripening tomato, this coincides with change regulation from auto-inhibitory to autostimulatory. The signaling pathways that operate bring about transition so-called system-1 system-2 are unknown, we have begun address these...
Fruit constitutes a major component of human diets, providing fiber, vitamins, and phytonutrients. Carotenoids are class compounds found in many fruits, nutritional benefits as precursors to essential vitamins antioxidants. Although recent gene isolation efforts metabolic engineering have primarily targeted genes involved carotenoid biosynthesis, factors that regulate flux through the pathway remain largely unknown. Characterization tomato high-pigment mutations ( hp1 hp2 ) suggests...
Pretty or Sweet The grocery-store tomato that looks beautiful but tastes like tart cardboard arises from selection processes favoring phenotypes make commercial production more reliable. Significant in process was a mutation reduced the mottled color variations of unripe green tomatoes, leaving them uniform, pale, green. Powell et al. (p. 1711 ) analyzed molecular biology mutation. uniform ripening turns out to disable transcription factor called Golden 2-like ( GLK2 ). expression increases...
The tomato ACC oxidase gene family is comprised of three members designated ACO1, ACO2 and ACO3 . These are highly homologous throughout the protein coding regions but do show a degree sequence divergence within 3′ untranslated regions. have been cloned used as gene‐specific probes to analyse differential expression in various tissues at different stages development. Results indicate that all genes transcriptionally active display high inducibility number organs life cycle. Both ACO1...
Petunia hybrida is a popular bedding plant that has long history as genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives its two wild parents, P. axillaris N inflata S6. The assemblies include 91.3% 90.2% coverage their diploid genomes (1.4 Gb; 2n = 14) containing 32,928 36,697 protein-coding genes, respectively. reveal lineage experienced at least rounds hexaploidization: older gamma event, which shared with most Eudicots, more recent Solanaceae...
Abstract Color changes often accompany the onset of ripening, leading to brightly colored fruits that serve as attractants seed-dispersing organisms. In many fruits, including tomato (Solanum lycopersicum) and pepper (Capsicum annuum), there is a sharp decrease in chlorophyll content concomitant increase synthesis carotenoids result conversion chloroplasts into chromoplasts. The green-flesh (gf) retainer (cl) mutations pepper, respectively, are inhibited their ability degrade during...
To achieve full ripening, climacteric fruits, such as tomato require synthesis, perception and signal transduction of the plant hormone ethylene. The nonripening phenotype dominant Green-ripe ( Gr ) Never-ripe 2 Nr-2 mutants is result reduced ethylene responsiveness in fruit tissues. In addition, a subset responses associated with floral senescence, abscission, root elongation are also impacted mutant plants, but to lesser extent. Using positional cloning, we have identified an identical...
In this paper we describe a novel, dominant pleiotropic tomato (Lycopersicon esculentum)-ripening mutation, Cnr (colorless nonripening). This mutant occurred spontaneously in commercial population. has phenotype that is quite distinct from of the other tomato-ripening mutants and characterized by fruit show greatly reduced ethylene production, an inhibition softening, yellow skin, nonpigmented pericarp. The ripening-related biosynthesis carotenoid pigments was abolished pericarp tissue. also...
Abstract We show that above a certain threshold concentration, ozone leads to leaf injury in tomato (Lycopersicon esculentum). Ozone-induced damage was preceded by rapid increase 1-aminocyclopropane-1-carboxylic acid (ACC) synthase activity, ACC content, and ethylene emission. Changes mRNA levels of specific synthase, oxidase, receptor genes occurred within 1 5 h. Expression the encoding components biosynthesis perception, biochemistry synthesis suggested ozone-induced is under biphasic...
Abstract Functional gene clusters, containing two or more genes encoding different enzymes for the same pathway, are sometimes observed in plant genomes, most often when specify synthesis of specialized defensive metabolites. Here, we show that a cluster tomato (Solanum lycopersicum; Solanaceae) contains terpene synthases (TPSs) monoterpenes and diterpenes from cis-prenyl diphosphates, substrates synthesized by encoded transferase (CPT) also located within cluster. The monoterpene synthase...
Plant epidermal cells have evolved specialist functions associated with adaptation to stress. These include the synthesis and deposition of specialized metabolites such as waxes cutin together flavonoids anthocyanins, which important roles in providing a barrier water loss protection against UV radiation, respectively. Characterization sticky peel (pe) mutant tomato (Solanum lycopersicum) revealed several phenotypes indicative defect cell function, including reduced anthocyanin accumulation,...
The chlorophyll content of unripe fleshy fruits is positively correlated with the nutrient and flavor ripe fruit. In tomato (Solanum lycopersicum) fruit, uniform ripening (u) locus, which encodes a GOLDEN 2-LIKE transcription factor (SlGLK2), influences gradient chloroplast development that extends from stem end fruit surrounding calyx to base With exception u factors influence formation this developmental are unknown. study, characterization positional cloning gray-green (ug) locus reveals...
Abstract Tropinone is the first intermediate in biosynthesis of pharmacologically important tropane alkaloids that possesses 8-azabicyclo[3.2.1]octane core bicyclic structure defines this alkaloid class. Chemical synthesis tropinone was achieved 1901 but mechanism has remained elusive. In study, we identify a root-expressed type III polyketide synthase from Atropa belladonna (AbPYKS) catalyzes formation 4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoic acid. This catalysis proceeds through...
The role of ethylene in regulating the expression three lipoxygenase (LOX) genes TomloxA, TomloxB and TomloxC during fruit ripening has been investigated. study used a combination low-ethylene containing an ACC oxidase (ACO1) sense-suppressing transgene tomato mutants deficient perception case Never-ripe (Nr) or ripening-inhibitor (rin). results indicate that are differentially regulated developmental factors influence their expression. TomloxA declines this is delayed ACO1 transgenic Nr...
Abstract The ripening of a fleshy fruit represents the summation an array biochemical processes that are regulated by interactions between developmental programs and environmental inputs. Analysis tomato (Solanum lycopersicum) mutants inhibitor studies indicate ethylene is necessary for full development program climacteric such as tomato, yet alone not sufficient. This suggests interaction nonethylene (or developmental) pathways mediates ripening. In this study, we have examined...
Abstract Acylsugars are polyesters of short- to medium-length acyl chains on sucrose or glucose backbones that produced in secretory glandular trichomes many solanaceous plants, including cultivated tomato (Solanum lycopersicum). Despite their roles biotic stress adaptation and wide taxonomic distribution, there is relatively little information about the diversity these compounds genes responsible for biosynthesis. In this study, acylsugar was assessed 80 accessions wild species Solanum...
Abstract The chloroplast is the site of photosynthesis in higher plants but also functions as center synthesis for primary and specialized metabolites including amino acids, fatty starch, diverse isoprenoids. Mutants that disrupt aspects function represent valuable tools defining structural biochemical regulation its interplay with whole-plant structure function. lutescent1 (l1) l2 mutants tomato (Solanum lycopersicum) possess a range chlorophyll-deficient phenotypes reduced rates...
A systematic screen of volatile terpene production in the glandular trichomes 79 accessions Solanum habrochaites was conducted and revealed presence 21 mono- sesquiterpenes that exhibit a range qualitative quantitative variation. Hierarchical clustering identified distinct phenotypic modules with shared patterns accumulation across accessions. Several could be assigned to previously synthase (TPS) activities included members TPS-e/f subfamily utilize unusual cis-prenyl diphosphate substrates...
The tropane alkaloids, hyoscyamine and scopolamine, are medicinal compounds that the active components of several therapeutics. Hyoscyamine scopolamine synthesized in roots specific genera Solanaceae a multistep pathway is only partially elucidated. To facilitate greater understanding alkaloid biosynthesis, de novo transcriptome assembly was developed for Deadly Nightshade (Atropa belladonna). Littorine key intermediate biosynthesis produced by condensation tropine phenyllactic acid....
Abstract Pollination of many flowers leads to an increase in ethylene synthesis and flower senescence. We have investigated the regulation pollination-induced tomato (Lycopersicon esculentum) using dialytic(dl) mutant, which pollination can be manipulated experimentally, with aim developing a model system study Ethylene increased rapidly indl pistils following pollination, leading accelerated petal senescence, was delayed ethylene-insensitiveNever-ripe (Nr) pistils. However,Nr eventually...