A5059497649- Pancreatic function and diabetes
- Organ Transplantation Techniques and Outcomes
- Plant Molecular Biology Research
- Renal Transplantation Outcomes and Treatments
- Diabetes and associated disorders
- Plant Reproductive Biology
- Cannabis and Cannabinoid Research
- Diabetes Management and Research
- Plant Gene Expression Analysis
- Liver physiology and pathology
- Clinical Nutrition and Gastroenterology
- Liver Disease and Transplantation
- Organ Donation and Transplantation
- Legume Nitrogen Fixing Symbiosis
- Xenotransplantation and immune response
- Pancreatitis Pathology and Treatment
- Plant Physiology and Cultivation Studies
- Appendicitis Diagnosis and Management
- DNA Repair Mechanisms
- Mycorrhizal Fungi and Plant Interactions
- Plant tissue culture and regeneration
- Minimally Invasive Surgical Techniques
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Abdominal Trauma and Injuries
- CRISPR and Genetic Engineering
Laboratoire Reproduction et Développement des Plantes
2013-2024
Centre National de la Recherche Scientifique
2008-2024
Université Claude Bernard Lyon 1
2008-2024
École Normale Supérieure de Lyon
2004-2024
Centre Hospitalier Universitaire Amiens-Picardie
2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2013-2023
University Hospital of Geneva
2008-2019
Hôpital Beau-Séjour
1993-2018
University of Geneva
1995-2017
Geneva College
2004-2017
Petunia hybrida is a popular bedding plant that has long history as genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives its two wild parents, P. axillaris N inflata S6. The assemblies include 91.3% 90.2% coverage their diploid genomes (1.4 Gb; 2n = 14) containing 32,928 36,697 protein-coding genes, respectively. reveal lineage experienced at least rounds hexaploidization: older gamma event, which shared with most Eudicots, more recent Solanaceae...
There is a crucial need for noninvasive assessment tools after cell transplantation. This study investigates whether magnetic resonance imaging (MRI) strategy could be clinically applied to islet The purest fractions of seven human preparations were labeled with superparamagnetic iron oxide particles (SPIO, 280 microg/mL) and transplanted into four patients type 1 diabetes. MRI studies (T2*) performed prior at various time points Viability in vitro vivo functions islets similar those control...
We report a comparative analysis of cell proliferation patterns during Arabidopsis flower development. Cell division was evaluated by direct method, i.e. the 5-bromo-2'-deoxyuridine (BrdU) incorporation/immunodetection procedure. BrdU in wild-type plants were correlated with expression profiles both several cycle genes involved control G(1)/S transition and cycle-related repressor genes, MSI4 MSI5, encoding WD-repeat proteins. To evaluate how arise respect to boundaries vice versa, boundary...
Abstract In Arabidopsis thaliana, flowers are determinate, showing a fixed number of whorls. Here, we report on three independent genes, novel gene REBELOTE (RBL; protein unknown function), SQUINT (SQN; cyclophilin), and ULTRAPETALA1 (ULT1; putative transcription factor) that redundantly influence floral meristem (FM) termination. Their mutations, combined with each other or crabs claw, the genetic background in which they were isolated, trigger strong FM indeterminacy reiterations extra...
Plant biology in general, and plant evo-devo particular would strongly benefit from a broader range of available model systems. In recent years, technological advances have facilitated the analysis comparison individual gene functions multiple species, representing now fairly wide taxonomic kingdom. Because genes are embedded networks, studying evolution function ultimately should be put context entire since changes single will normally go together with further its network environment. For...
Arbuscular mycorrhiza (AM) is a mutual symbiosis that involves complex symbiotic interface over which nutrients are exchanged between the plant host and AM fungus. Dozens of genes in required for establishment functioning interaction, among them nutrient transporters mediate uptake mineral delivered by fungal arbuscules. We have isolated genetic mutant screen petunia (Petunia hybrida) GIBBERELLIC ACID INSENSITIVE, REPRESSOR SCARECROW (GRAS)-type transcription factor, ATYPICAL ARBUSCULE...
Bacterial lipo-chitooligosaccharides (LCOs) are key mediators of the nitrogen-fixing root nodule symbiosis (RNS) in legumes. The isolation LCOs from arbuscular mycorrhizal fungi suggested that also signaling molecules mycorrhiza (AM). However, corresponding plant receptors have remained uncharacterized. Here we show petunia and tomato mutants LysM receptor-like kinases LYK10 impaired AM formation. Petunia proteins a high affinity for (Kd nM range) comparable to previously reported legume LCO...
Transplantation is the treatment of choice for children with end-stage renal disease. However, long-term quality life and socioprofessional outcome those successful transplants have not previously been reported. We studied these factors in patients transplanted when<18 years old who currently ≥10 graft function. A total 57 questionnaires were sent out; (100%) responded [24 female 33 male patients; average (±SD) age at tx = 10±5 (0.9–17.7); f/u 15.6±3 (10–26); current 26±5 (12–38); 26 had < 1...
High-level expression of a chimeric virA gene was obtained by replacing the first 524 codons with half trpE. The encoded fusion protein isolated and found to exhibit autokinase activity. Therefore, kinase domain is in C-terminal portion VirA, phosphorylation may be an important feature VirA function.
Higher plants continuously and iteratively produce new above-ground organs in the form of leaves, stems flowers. These arise from shoot apical meristems whose homeostasis depends on coordination between self-renewal stem cells their differentiation into organ founder cells. This is stringently controlled by central transcription factor WUSCHEL (WUS), which both necessary sufficient for cell specification Arabidopsis thaliana ULTRAPETALA1 (ULT1) was previously identified as a plant-specific,...
To attract insects, flowers produce nectar, an energy-rich substance secreted by specialized organs called nectaries. For Arabidopsis thaliana, a rosid species with stamen-associated nectaries, the floral B-, C-, and E-functions were proposed to redundantly regulate nectary development. Here, we investigated molecular basis of carpel-associated development in asterid petunia (Petunia hybrida). We show that its euAGAMOUS (euAG) PLENA (PLE) C-lineage MADS box proteins are essential for...
Members of SEPALLATA (SEP) and APETALA1 (AP1)/SQUAMOSA (SQUA) MADS-box transcription factor subfamilies play key roles in floral organ identity determination meristem determinacy the rosid species Arabidopsis (Arabidopsis thaliana). Here, we present a functional characterization seven SEP/AGL6 four AP1/SQUA genes distant asterid petunia (Petunia × hybrida). Based on analysis single higher order mutants, report that SEP1/SEP2/SEP3 orthologs together with AGL6 encode classical SEP termination...
PURPOSE There is no consensus on the treatment of patients with Waldenström's macroglobulinemia (WM) who develop primary or secondary resistance to frontline therapies. We report our experience activity and toxicity fludarabine in 71 WM resistant prior chemotherapy regimens. PATIENTS AND METHODS From January 1991 June 1995, were included this retrospective study. The median age, time from diagnosis treatment, immunoglobulin M (IgM) level, number previous treatments 68 years (range, 42 81),...
The aim of this study was to assess the efficiency and safety Edmonton immunosuppression protocol in recipients islet-after-kidney (IAK) grafts. Fifteen islet infusions were administered 8 patients with type 1 diabetes a functioning kidney graft. Immunosuppression switched on day transplantation regimen associating sirolimus-tacrolimus-daclizumab. Insulin-independence achieved all for at least 3 months, an actual rate 71% year after (5 7 patients). After 24-month mean follow-up, five have...
The product of the uvrD gene Escherichia coli, UvrD (helicase II), is known to be involved in methyl-directed mismatch repair, transposon excision and uvrABC repair. In conjugational crosses, various mutants have been reported result higher, lower or unaffected recombination frequencies. an attempt clarify role recombination, we studied vitro its effects on two key reactions driven by RecA, homologous pairing strand exchange. We show here that efficiently prevents reverses RecA-mediated...
Summary The virulence regulon of the Agrobacterium tumefaciens TiC58 plasmid is composed six operons, virA, virB, virG, virC, virD and virE , which direct transfer T‐DNA into plant cells. 9.5kbp virB operon largest these operons its entire nucleotide sequence was determined found to contain eleven open reading frames (ORFs). Gene fusions each VirB ORF T7φ10 were made over‐expressed in Escherichia coli confirm that they encode proteins predicted size. Hydrophobic analysis peptide sequences...
Rigorous assessment of health-related quality life (HRQL) is mandatory to establish the benefits islet transplantation.The 36-Item Short Form Health Survey (SF-36) and Diabetes Quality Life (DQOL) scales were completed by patients included in an Islet Transplantation Alone (ITA) trial (n = 10) After Kidney (IAK) 10).The two populations differed HRQL scores at baseline, with poorer ITA patients. SF-36 for physical limitations, bodily pain, general health perception, social functioning,...