A5072346837- Plant Molecular Biology Research
- Plant and animal studies
- Plant Reproductive Biology
- Light effects on plants
- Botanical Research and Applications
- Postharvest Quality and Shelf Life Management
- Metabolomics and Mass Spectrometry Studies
- Agricultural pest management studies
- Plant Physiology and Cultivation Studies
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Algal biology and biofuel production
- Plant Disease Resistance and Genetics
- Plant Parasitism and Resistance
- Flowering Plant Growth and Cultivation
- Vocational Education and Training
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Plant Pathogens and Resistance
- Transgenic Plants and Applications
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Plant and soil sciences
- Greenhouse Technology and Climate Control
- Genetic and Environmental Crop Studies
Universidad Politécnica de Cartagena
2015-2025
Emmes (United States)
2022-2025
Leibniz Institute for Catalysis
2024
GESIS - Leibniz-Institute for the Social Sciences
2023
Aschaffenburg University of Applied Sciences
2023
Hôpital Civil, Strasbourg
2020
Université de Lorraine
2019
Instituto de Fisiología Vegetal
2009-2018
Paderborn University
2016-2018
Karlsruhe Institute of Technology
2017
Summary Late blight caused by the oomycete Phytophthora infestans is most destructive disease in potato cultivation worldwide. New, more virulent P. strains have evolved which overcome genetic resistance that has been introgressed conventional breeding from wild species into commercial varieties. R genes (for single‐gene resistance) and for quantitative to late are present germplasm of cultivated potato. The molecular basis unknown. We cloned R1, first gene blight, combining positional...
Petunia hybrida is a popular bedding plant that has long history as genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives its two wild parents, P. axillaris N inflata S6. The assemblies include 91.3% 90.2% coverage their diploid genomes (1.4 Gb; 2n = 14) containing 32,928 36,697 protein-coding genes, respectively. reveal lineage experienced at least rounds hexaploidization: older gamma event, which shared with most Eudicots, more recent Solanaceae...
Abstract Background Identification of genes with invariant levels gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally these should be independent the morphogenetic process or environmental condition tested as well methods used RNA purification and analysis. Results In an effort to identify endogenous meeting criteria nine reference (RG) were in two Petunia lines (Mitchell V30). Growth conditions differed Mitchell V30, different isolation...
Abstract We present an integrated analysis of the molecular repertoire Chlamydomonas reinhardtii under reference conditions. Bioinformatics annotation methods combined with GCxGC/MS-based metabolomics and LC/MS-based shotgun proteomics profiling technologies have been applied to characterize abundant proteins metabolites, resulting in detection 1069 159 metabolites. Of measured proteins, 204 currently do not EST sequence support; thus a significant portion proteomics-detected provide...
With its controlled environment, protected cultivation is advantageous and effective for breeding programs. This distinct setting also guarantees that fresh vegetables meet high quality standards. The environment allows precise monitoring tuning of efforts, a critical factor in continuously improving the vegetable production. Classical strategies include hybridization, pedigree selection, backcrossing, recombination, marker-assisted breeding. However, advanced techniques like phenomics...
In the era of fast genome sequencing a critical goal is to develop genome-wide quantitative molecular approaches. Here, we present metaproteogenomic strategy integrate proteomics and metabolomics data for systems level analysis in recently sequenced unicellular green algae Chlamydomonas reinhardtii. To achieve representative proteome coverage analysed different growth conditions with protein prefractionation shotgun proteomics. For identification, annotations as well new gene model...
Crop-plant-yield safety is jeopardized by temperature stress caused the global climate change. To take countermeasures breeding and/or transgenic approaches it essential to understand mechanisms underlying plant acclimation heat stress. this end proteomics are most promising, as largely mediated proteins. Accordingly, several studies, mainly based on two-dimensional gel-tandem MS approaches, were conducted in past. However, results often inconsistent, presumably attributable artifacts...
Ever since agriculture started, plants have been bred to obtain better yields, fruits, or sustainable products under uncertain biotic and abiotic conditions. However, a new way from plant cells emerged with the development of recombinant DNA technologies. This led possibility producing exogenous molecules in plants. Furthermore, chemodiversity has main source pharmacological molecules, opening field biotechnology directed produce high quality metabolites. The need for different by pharma,...
The reproductive biology of the climbing cacti Hylocereus polyrhizus (Weber) Britt. & Rose, H. undatus (Haworth) costaricensis and Selenicereus megalanthus (Schum. ex Vaupel) Moran (syn. Mediocactus ) was studied with aim cultivating in Israel as fruit crops. Flowering spp. occurred two to three waves during summer, whereas S. , flowering concentrated at end autumn. Flowers all species opened 1 1.5 hours before sunset closed ≈6 after sunrise. In spp., were self-unfruitful,...
Relative calculation of differential gene expression in quantitative PCR reactions requires comparison between amplification experiments that include reference genes and under study. Ignoring the differences their efficiencies may lead to miscalculation even with same starting amount template. Although there are several tools performing primer design, is no tool available predicts efficiency for a given amplicon pair. We have used statistical approach based on 90 pair combinations amplifying...
Abstract BACKGROUND: Cherry tomato fruits cv. Micro‐Tom, a model plant for genetics, were analysed in order to determine the main structural and chemical changes under optimal chilling storage conditions. The comparison of Micro‐Tom standard cultivars will give an insight into suitability this dwarf cultivar as studying influence low‐temperature conditions on fruits. RESULTS: During chilling, fruit tissue was progressively destroyed due collapse deep layers pericarp. Chilling lowered typical...
Full scent profiles emitted by living tissues can be screened using total ion chromatograms generated in full scan mode and gas chromatography-mass spectrometry technique Headspace Sorptive Extraction. This allows the identification of specific compounds their absolute quantification or relative abundance. Quantifications ideally should based on calibration curves standards for each compound. However, unpredictable composition Volatile Organic Compounds (VOCs) lack make this approach...
The prevalence and severity of latex allergy has increased dramatically in the last 15 years due to exposure natural rubber products. Although historically this health risk been elevated hospital personnel patients, a recent survey indicated significant potential for general population. To obtain wide-spread source exposure, we have considered tire debris. We searched presence allergens passenger car truck tread, debris deposited from atmosphere near freeway, airborne particulate matter...
Phenomics is a technology-driven approach with promising future to obtain unbiased data of biological systems. Image acquisition relatively simple. However handling and analysis are not as developed compared the sampling capacities. We present system based on machine learning (ML) algorithms computer vision intended solve automatic phenotype in plant material. growth-chamber able accommodate species various sizes. Night image requires near infrared lightning. For ML process, we tested three...
Multiple emulsions of the W1/O/W2 type are promising tools for encapsulating bioactive ingredients in inner aqueous droplets. It is necessary, however, to control factors influencing their encapsulation efficiency. One important factor particle size because it determines surface area available mass transport. Because coexistence water and oil droplets multiple emulsions, there numerous that have an impact on size, example, phase composition, interfacial properties, viscosity phases. The...
Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) specific Immunglobulin (Ig) E-levels. Isolated tomato total protein showed high SPT activity comparable or even higher than protein. For the molecular characterization of allergens, multidimensional fractionation strategy LC-MS/MS was used. Two legumin- vicilin-proteins purified strong IgE-reactivity in immunoblots....
The cactus Opuntia ficus-indica is a constitutive Crassulacean acid metabolism (CAM) species. Current knowledge of CAM suggests that the enzyme phosphoenolpyruvate carboxylase kinase (PPCK) circadian regulated at transcriptional level, whereas (PEPC), malate dehydrogenase (MDH), NADP-malic (NADP-ME), and pyruvate phosphate dikinase (PPDK) are posttranslationally controlled. As little transcriptomic data available from obligate plants, we created an expressed sequence tag database derived...
The transcriptional network topology of B function in Antirrhinum, required for petal and stamen development, is thought to rely on initial activation transcription DEFICIENS (DEF) GLOBOSA (GLO), followed by a positive autoregulatory loop maintaining gene expression levels. Here, we show that the mutant compacta (co), whose vegetative growth size are affected, plays role function. Late events morphogenesis such as development conical cell area scent emissions were reduced co def...