A5080264896- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant Micronutrient Interactions and Effects
- Photosynthetic Processes and Mechanisms
- Postharvest Quality and Shelf Life Management
- Mycorrhizal Fungi and Plant Interactions
- Legume Nitrogen Fixing Symbiosis
- Cassava research and cyanide
- Peroxisome Proliferator-Activated Receptors
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Genetic and Environmental Crop Studies
- SARS-CoV-2 and COVID-19 Research
- Fungal Biology and Applications
- Plant Surface Properties and Treatments
- Aluminum toxicity and tolerance in plants and animals
- Virus-based gene therapy research
- Plant tissue culture and regeneration
- Saffron Plant Research Studies
- CAR-T cell therapy research
- Seedling growth and survival studies
- Seed Germination and Physiology
- Plant Physiology and Cultivation Studies
- Horticultural and Viticultural Research
Instituto de Biología Molecular y Celular de Plantas
2015-2024
Consejo Superior de Investigaciones Científicas
2015-2024
Universitat Politècnica de València
2015-2024
Hospital Universitari i Politècnic La Fe
2005-2023
Hospital Universitario Reina Sofía
2023
University of Castilla-La Mancha
2012
Estación Experimental del Zaidín
1986-2011
Centro de Investigación en Materiales Avanzados
1996
Polytechnic University of Puerto Rico
1996
The effect of growing pea (Pisum sativum L.) plants with CdCl2 (0–50 μM) on different plant physiological parameters and antioxidative enzymes leaves was studied in order to know the possible involvement this metal generation oxidative stress. In roots Cd produced a significant inhibition growth as well reduction transpiration photosynthesis rate, chlorophyll content leaves, an alteration nutrient status both leaves. ultrastructural analysis from grown 50 μM CdCl2, showed cell disturbances...
ABSTRACT Cadmium is a toxic metal that produces disturbances in plant antioxidant defences giving rise to oxidative stress. The effect of this on H 2 O and ·− production was studied leaves from pea plants growth for weeks with 50 µ m Cd, by histochemistry diaminobenzidine (DAB) nitroblue tetrazolium (NBT), respectively. subcellular localization these reactive oxygen species (ROS) cytochemistry CeCl 3 Mn/DAB staining , respectively, followed electron microscopy observation. In grown CdCl six...
Abstract In pea ( Pisum sativum L.) leaves from plants grown in the presence of 50 µ m CdCl 2 oxidative production carbonyl groups proteins, rate protein degradation and proteolytic activity were investigated. leaf extracts content measured by derivatization with 2,4‐dinitrophenylhydrazine (DNPH), was two‐fold higher treated Cd than control plants. The identification oxidized proteins carried out sodium dodecyl sulphate‐polyacrylamide gel electrophoresis derivatized DNPH immunochemical...
Summary Successful plant survival depends upon the proper integration of information from environment with endogenous cues to regulate growth and development. We have investigated interplay between ambient temperature hormone action during regulation hypocotyl elongation, we found that gibberellins (GAs) auxin are quickly independently recruited by modulate rate, whereas activity brassinosteroids (BRs) seems be required later on. Impairment GA biosynthesis blocked increased elongation caused...
The tolerance of lettuce plants (Lactuca sativa L. cv. Romana) to drought stress differed with the arbuscular-mycorrhizal fungal isolate which were associated. Seven species belonging genus Glomus studied for their ability enhance plants. These fungi had different traits that affected resistance host ranking effects on tolerance, based relative decreases in shoot dry weight, was as follows: deserticola > fasciculatum mosseae etunicatum intraradices caledonium occultum. In this comparative...
Saffron, the processed stigma of <i>Crocus sativus</i>, is characterized by presence several apocarotenoids that contribute to color, flavor, and aroma spice. However, little known about synthesis compounds during development <i>C. sativus</i> stigma. The developing nearly odorless, but before at anthesis, aromatic compound β-ionone becomes principal norisoprenoid volatile in In this study, four carotenoid cleavage dioxygenase (CCD) genes, <i>CsCCD1a</i>, <i>CsCCD1b</i>, <i>CsCCD4a</i>,...
Improved salt tolerance of mycorrhizal plants is commonly attributed to their better mineral nutrition, particularly phosphorus. However, the effect arbuscular‐mycorrhizal (AM) fungi on may not be limited this mechanism. We investigated possibility that non‐nutritional effects AM fungi, based proline accumulation or increased photosynthesis and related parameters, can influence lettuce ( Lactuca sativa L.) salinity. Three levels (3, 4 5 g NaCl kg ‐1 dry soil) were applied maintained under...
Abscission is a cell separation process by which plants can shed organs such as fruits, leaves, or flowers. The takes place in specific locations termed abscission zones. In fruit crops like citrus, represents high percentage of annual yield losses. Thus, understanding the molecular regulation capital relevance to control production. To identify genes preferentially expressed within citrus zone (AZ-C), we performed comparative transcriptomics assay at type resolution level between AZ-C and...
Keywords: Ascorbate peroxidaseCadmiumGlutathione reductaseHydrogen peroxideOxidative stressPeroxisomesAbbreviations: APX, ascorbate peroxidaseCAT, catalaseDAB, diaminobenzidineEM, electron microscopyCOX, glyeolate oxidaseGR, glutathione reductaseG6PD11, glucose 6-phosphate dehydrogenaseIEF, isoelectric focusingICDII, isocitrate dehydrogenaseMDA, malonaldehydeMDHAK, monodehydroascorbate reductaseNUT, nitro blue tetrazoliumPAGE, polyacrylamide gel electrophoresis6PGDI I, b-phosphogluconate...
ABSTRACT 2,4‐dichlorophenoxyacetic acid (2,4‐D) is an analogue compound to the plant hormone indole‐3‐acetic (IAA), which used either as a growth‐promoting substance or herbicide, depending on its concentration. In this work, effect of 2,4‐D growth and ROS metabolism pea ( Pisum sativum L.) leaves reported. The herbicide considerably reduced negatively influenced several physiological parameters in dose‐dependent manner. At structural level, damage mesophyll cells enlargement dilation...
Summary Gibberellins ( GA s) play a critical role in fruit‐set and fruit growth. Gibberellin is perceived by its nuclear receptors INSENSITIVE DWARF 1s GID 1s), which then trigger degradation of downstream repressors DELLA s. To understand the three receptor genes 1A , 1B 1C ) Arabidopsis during initiation, we have examined their temporal spatial localization, combination with analysis mutant phenotypes. Distinct expression patterns are revealed for each 1: expressed throughout whole pistil,...
Ovule formation is a complex developmental process in plants with strong impact on the production of seeds. primordia initiation controlled by gene network, including components signaling pathways auxin, brassinosteroids and cytokinins. In contrast, gibberellins (GAs) their negative regulators DELLA proteins have never been shown to be involved ovule initiation. Here we provide molecular genetic evidences that point as novel players determination number Arabidopsis species agronomic interest...
Abstract This study compared standard of care testing (SOC) to BioFire® FilmArray® Pneumonia plus Panel (PN ). PN detects 15 bacteria with semiquantitative log bin values, 7 antibiotic resistance markers, three atypical (AB), and eight viral classes directly from bronchoalveolar lavage-like specimens (BLS) sputum-like (SLS). Fifty-two laboratories 13 European countries Israel tested 1234 BLS 1242 SLS SOC. Detection rates number pathogens/samples were for pathogens. values SOC quantities...
Abstract The effect of growing pea ( Pisum sativum L.) plants with a toxic CdCl 2 concentration (50 µ m ) on the metabolism and proteolytic activity leaf peroxisomes was studied. In purified from treated cadmium, an increase in total protein level photorespiratory enzyme glycolate oxidase found. glyoxylate cycle enzymes, malate synthase isocitrate lyase, whose is normally very low peroxisomes, were enhanced by Cd treatment. endogenous proteases determined. Two leucine‐aminopeptidase isozymes...
Abstract UGT707B1 is a new glucosyltransferase isolated from saffron (Crocus sativus) that localizes to the cytoplasm and nucleus of stigma tepal cells. transcripts were detected in tissue all Crocus species analyzed, but expression analysis tepals revealed its absence certain species. The glucosylated flavonoids present reveals presence two major flavonoid compounds saffron: kaempferol-3-O-β-d-glucopyranosyl-(1-2)-β-d-glucopyranoside...
Gibberellins (GAs) are plant hormones that regulate most life cycle aspects, including flowering and fruit development. Here, we demonstrate the implication of GAs in ovule DELLA proteins, negative GA response regulators, act as positive factors for integument development a mechanism involves transcription factor ABERRANT TESTA SHAPE (ATS). The seeds della global mutant, complete loss-of-function DELLA, ats-1 mutant remarkably similar, with round shape, disorganized testa, viviparism. These...
SUMMARY Ovule development is a key process for plant reproduction, helping to ensure correct seed production. Several molecular factors and hormones such as gibberellins are involved in ovule initiation development. Gibberellins control by the destabilization of DELLA proteins, whereas activity has been shown act positive factor primordia emergence. But mechanism which acts remained unknown. In this study we report that proteins participate formation protein complex with CUC2 transcription...
This paper describes the isolation and characterization of LRP , a new gene from tomato plants. The deduced amino acid sequence showed that encoded protein is enriched in leucine, contains interesting structural motifs. four tandem repeats canonical 24 leucine‐rich repeat (LRR) present different proteins mediates molecular recognition and/or interaction processes. Genomic organization intron‐exon arrangement favor hypothesis LRR domains evolved by exon duplication shuffling. expression...
summary Plants of Lactuca sativa L. cv. Romana were inoculated with two vesicular‐arbuscular (VA) mycorrhizal fungi, Glomus mosseae (Nicol. & Gerd.) Gerd. Trappe and fasciculatum (Thaxter sensu Trappe, or provided phosphorus. These three treatments plus a control supplemented 3.0 mM nitrogen as nitrate ammonium in 1 5 NO 3 − /NH 4+ ratios. Leaf root weights, N, P, K, Ca Mg absorption, well the percentage length colonized, evaluated. Physiological parameters such leaf area, photosynthetic...
Ovule primordia formation is a complex developmental process with strong impact on the production of seeds. In Arabidopsis this controlled by gene network, including components signalling pathways auxin, brassinosteroids (BRs) and cytokinins. Recently, we have shown that gibberellins (GAs) also play an important role in ovule initiation, inhibiting both tomato. Here reveal BRs participate control initiation tomato, promoting increase formation. Moreover, molecular genetic analyses...
During grape ripening, numerous transcriptional and metabolic changes are required in order to obtain colored, sweet, flavored berries. There is evidence that ethylene, together with other signals, plays an important role triggering the onset of ripening. Here, we report functional characterization a berry-specific Ethylene Responsive Factor (ERF), VviERF045, which induced just before véraison peaks at Phylogenetic analysis revealed it close SHINE clade ERFs, factors involved regulation wax...