A5034979393- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Hemoglobin structure and function
- Antimicrobial Resistance in Staphylococcus
- Erythrocyte Function and Pathophysiology
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Hemoglobinopathies and Related Disorders
- Neonatal Health and Biochemistry
- Infective Endocarditis Diagnosis and Management
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Heme Oxygenase-1 and Carbon Monoxide
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Mass Spectrometry Techniques and Applications
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Sepsis Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Listeria monocytogenes in Food Safety
- Otolaryngology and Infectious Diseases
- Genomics and Phylogenetic Studies
- Adipose Tissue and Metabolism
- Reproductive tract infections research
Hôpital Cochin
2016-2025
Assistance Publique – Hôpitaux de Paris
2016-2025
Groupe Hospitalier Cochin - Port-Royal, Hôtel-Dieu, Broca - La Collégiale
2014-2024
Laboratoire National de Référence
2015-2024
Inserm
2015-2024
Université Paris Cité
2015-2024
Institut Cochin
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2022-2024
Sorbonne Paris Cité
2012-2024
Summary Streptococcus agalactiae is a commensal bacterium colonizing the intestinal tract of significant proportion human population. However, it also pathogen which leading cause invasive infections in neonates and causes septicaemia, meningitis pneumonia. We sequenced genome serogroup III strain NEM316, responsible for fatal case septicaemia. The 2 211 485 base pairs long contains 2118 protein coding genes. Fifty‐five per cent predicted genes have an ortholog pyogenes genome, representing...
Immunoproliferative small intestinal disease (also known as alpha chain disease) is a form of lymphoma that arises in mucosa-associated lymphoid tissue (MALT) and associated with the expression monotypic truncated immunoglobulin heavy without an light chain. Early-stage responds to antibiotics, suggesting bacterial origin. We attempted identify causative agent.We performed polymerase reaction (PCR), DNA sequencing, fluorescence situ hybridization, immunohistochemical studies on...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotophysics and reactivity of heme proteins: a femtosecond absorption study hemoglobin, myoglobin, protohemeJ. W. Petrich, C. Poyart, J. L. MartinCite this: Biochemistry 1988, 27, 11, 4049–4060Publication Date (Print):May 31, 1988Publication History Published online1 May 2002Published inissue 31 1988https://pubs.acs.org/doi/10.1021/bi00411a022https://doi.org/10.1021/bi00411a022research-articleACS PublicationsRequest reuse permissionsArticle...
Streptococcus agalactiae (group B streptococcus; GBS) is a normal constituent of the intestinal microflora and major cause human neonatal meningitis. A single clone, GBS ST-17, strongly associated with deadly form infection called late-onset disease (LOD), which characterized by meningitis in infants after first week life. The pathophysiology LOD remains poorly understood, but our epidemiological histopathological results point to an oral route infection. Here, we identify novel...
Abstract Streptococcus agalactiae (Group B , GBS) is a commensal of the digestive and genitourinary tracts humans that emerged as leading cause bacterial neonatal infections in Europe North America during 1960s. Due to lack epidemiological genomic data, reasons for this emergence are unknown. Here we show by comparative genome analysis phylogenetic reconstruction 229 isolates rise human GBS corresponds selection worldwide dissemination only few clones. The parallel expansion clones preceded...
Abstract The vaginal microbiota refers to the microorganisms that reside in vagina. These contribute significantly a woman’s reproductive and general health. A healthy is typically low-diversity environment with predominance of lactic acid-producing Lactobacillus species. Factors such as antibiotic use, sexual activity, hormonal changes can disrupt balance microbiota, leading conditions bacterial vaginosis. composition takes on added importance during pregnancy, serving barrier against...
ABSTRACT We have used a PCR assay based on the use of degenerate primers in order to characterize an internal fragment ( sodA int ) representing approximately 85% genes encoding manganese-dependent superoxide dismutase various streptococcal type strains S. acidominimus , agalactiae alactolyticus anginosus bovis constellatus canis cricetus downei dysgalactiae equi subsp. zooepidemicus equinus gordonii iniae intermedius mitis mutans oralis parasanguis pneumoniae porcinus pyogenes salivarius...
ABSTRACT Simple PCR and sequencing assays that utilize a single pair of degenerate primers were used to characterize 429-bp-long DNA fragment internal ( sodA int ) the gene encoding manganese-dependent superoxide dismutase in 40 coagulase-negative staphylococcal (CNS) type strains. The topology phylogenetic tree obtained was general agreement with which inferred from an analysis their 16S rRNA or hsp60 sequences. Sequence revealed genes exhibit higher divergence than does corresponding...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHemoglobin as a receptor of drugs and peptides: x-ray studies the stereochemistry bindingMax F. Perutz, Giulio. Fermi, Donald J. Abraham, Claude. Poyart, E. BursauxCite this: Am. Chem. Soc. 1986, 108, 5, 1064–1078Publication Date (Print):March 1, 1986Publication History Published online1 May 2002Published inissue 1 March 1986https://pubs.acs.org/doi/10.1021/ja00265a036https://doi.org/10.1021/ja00265a036research-articleACS PublicationsRequest reuse...
The dlt operon of Gram-positive bacteria comprises four genes (dltA, dltB, dltC and dltD) that catalyse the incorporation D-alanine residues into cell wall-associated lipoteichoic acids (LTAs). In this work, we characterized Listeria monocytogenes constructed a D-Ala-deficient LTA mutant by inactivating first gene (dltA) operon. DltA- did not show any morphological alterations its growth rate was similar to wild-type strain. However, it exhibited an increased susceptibility cationic peptides...
We developed a simple, specific, and sensitive two-multiplex-PCR assay that enabled the detection of all known group B streptococcal (GBS) capsular polysaccharides. This test is well adapted for GBS polysaccharide typing in large-scale epidemiological studies.
We have identified an unusual group of viridans streptococci that resemble Streptococcus pneumoniae. DNA-DNA homology studies suggested a subset these isolates represent novel species may be included in the S. oralis-S. mitis streptococci. suggest this termed pseudopneumoniae. A combination phenotypic and genetic reactions allows its identification. pseudopneumoniae strains do not pneumococcal capsules, are resistant to optochin (inhibition zones, less than 14 mm) when they incubated under...
Summary Streptococcus agalactiae [group B streptococcus (GBS)] is the leading cause of neonatal pneumonia, sepsis and meningitis. An in silico genome analysis indicated that GBS strain NEM316 encodes five putative sortases, including major class A sortase enzyme four C sortases. The genes encoding sortases are tandemly arranged two different loci, srtC1‐C2 srtC3‐C4 , with a similar genetic organization thought to be involved pilus biosynthesis. Each pair flanked by LPXTG protein genes,...
FixL's are chimeric heme protein kinases from symbiotic nitrogen-fixing Rhizobia. We have overexpressed three FixL variants in Escherichia coli. Bradyrhizobium japonicum FixL, a soluble dimeric protein, is the first full-length to be purified. The other two proteins truncations of Rhizobium meliloti which membrane protein. One contains both and kinase domains dimeric; has only domain monomeric. find that all bind oxygen carbon monoxide non-cooperatively, with very low affinities due entirely...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLigand binding and protein relaxation in heme proteins: a room temperature analysis of nitric oxide geminate recombinationJ. W. Petrich, J. C. Lambry, K. Kuczera, Martin Karplus, Poyart, L. MartinCite this: Biochemistry 1991, 30, 16, 3975–3987Publication Date (Print):April 23, 1991Publication History Published online1 May 2002Published inissue 23 April...
Summary In this study, we carried out a detailed structural and functional analysis of Streptococcus agalactiae (GBS) two‐component system which is orthologous to the CovS/CovR (CsrS/CsrR) regulatory pyogenes . GBS, covR covS are part seven gene operon transcribed from two promoters that not regulated by CovR. A ΔcovSR mutant was found display dramatic phenotypic changes such as increased haemolytic activity reduced CAMP on blood agar. Adherence epithelial cells greatly transmission electron...
Human beta-globin was synthesized in Escherichia coli as a cleavable fusion protein, using the expression vector pLcIIFX [Nagai, K. & Thøgersen, H. C. (1984) Nature (London) 309, 810-812]. The protein cIIFX purified to homogeneity and cleaved at junction by blood coagulation factor Xa; authentic liberated. Beta-globin folded vitro reconstituted with heme alpha subunits form 2 beta tetramers. oxygen binding properties of Hb are essentially same those human native Hb. Two mutant Hbs (Hb...