A5046808910- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Phytochemicals and Antioxidant Activities
- Plant biochemistry and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Nuts composition and effects
- Plant Physiology and Cultivation Studies
- Essential Oils and Antimicrobial Activity
- Tea Polyphenols and Effects
- Plant Pathogens and Fungal Diseases
- Antioxidant Activity and Oxidative Stress
- Edible Oils Quality and Analysis
- Pomegranate: compositions and health benefits
- Diet and metabolism studies
- Mycotoxins in Agriculture and Food
- Food Quality and Safety Studies
- Botanical Research and Chemistry
- Berry genetics and cultivation research
- Advanced Neuroimaging Techniques and Applications
- Sirtuins and Resveratrol in Medicine
- Spectroscopy and Chemometric Analyses
- Traditional Chinese Medicine Analysis
- Analytical Chemistry and Chromatography
Fondazione Edmund Mach
2016-2025
Casa di Cura San Michele
2017
Joint Research Centre
2006
Compelling evidence of the health benefits phenolic compounds and their impact on food quality have stimulated development analytical methods for identification quantification these in different matrices recent years. A targeted metabolomics method has been developed 135 phenolics, such as benzoates, phenylpropanoids, coumarins, stilbenes, dihydrochalcones, flavonoids, fruit tea extracts wine using UPLC/QqQ-MS/MS. Chromatography was optimized to achieve separation over a period 15 min, MRM...
The main metabolites of caffeic and ferulic acids (ferulic acid-4'-O-sulfate, acid-3'-O-sulfate), the most representative phenolic in fruits vegetables, acyl glucuronide acid were synthesized, purified, tested for their antioxidant activity comparison with those parent compounds other related phenolics. Both ferric reducing power (FRAP) assay 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging method used. Ferulic acid-4'-O-sulfate acid-4'-O-glucuronide exhibited...
Background and Aims: Red wine is a very rich source of flavanols, consisting catechins oligomeric polymeric proanthocyanidins (PAs). The aim this study was to investigate the composition fraction which extractable from grape into wine. Methods Results: Using HPLC-DAD-MS analysis carried out before after thioacidolysis wine-like extracts obtained skins seeds, flavanol 20 samples derived Cabernet Sauvignon, Carmenere, Marzemino, Merlot, Pinot Noir, Syrah Teroldego determined. Conclusions: It...
Different polyphenol compounds are ingested when consuming a serving of fruits rich in polyphenols, spanning from one-phenol hydroxybenzoic acid to more complex polymeric compounds. Only minor quantity the polyphenols (5-10%) is absorbed. The remainder reaches colon and extensively metabolized by gut microbiota low-molecular weight metabolites. Their subsequent tissue distribution still undefined, although these microbial metabolites currently believed play role human health disease states....
Accurate quantification and structural characterization of ellagitannins ellagic acid conjugates in food, beverages, food supplements are essential starting points for studying their effect on human health. However, accuracy is hindered both by the lack pure standard compounds methods that maintain native form, avoiding any chemical modification structure. The objective this work was to develop a new method purification, chromatographic separation, accurate provide thorough diversity...
Gibberellins (GAs) are involved in the regulation of flowering and fruit-set grapes (Vitis vinifera L.), but molecular mechanisms behind this process mostly unknown. In work, family grapevine GA oxidases biosynthesis deactivation GAs was characterized. Six putative 20-oxidase (GA20ox), three 3-oxidase (GA3ox), eight 2-oxidase (GA2ox) proteins, latter further divided into five C19-GA 2ox C20-GA2ox were identified. Phylogenetic analyses suggest a common origin GA3ox C19-GA2ox groups challenge...
In the Vitaceae, viniferins represent a relatively restricted group of trans-resveratrol oligomers with antifungal properties, thus enabling plants to cope pathogen attack. The aim this study was perform isolation and structural characterization whole class accumulating in leaves hybrid Vitis vinifera (Merzling × Teroldego) genotypes infected Plasmopara viticola . Infected resistant were collected 6 days after infection, extracted methanol, prepurified by flash chromatography using ENV+...
In terms of the quality minimally processed fruit, flesh browning is fundamentally important in development an aesthetically unpleasant appearance, with consequent off-flavours. The depends on enzymatic action polyphenol oxidase (PPO). 'Golden Delicious' apple genome ten PPO genes were initially identified and located three main chromosomes (2, 5 10). Of these genes, one element particular, here called Md-PPO, chromosome 10, was further investigated genetically mapped two progenies ('Fuji x...
Wine micro-oxygenation is a globally used treatment and its effects were studied here by analysing untargeted LC-MS the wine metabolomic fingerprint. Eight different procedural variations, marked addition of oxygen (four levels) iron (two applied to Sangiovese wine, before after malolactic fermentation. Data analysis using supervised unsupervised multivariate methods highlighted some known candidate biomarkers, together with number metabolites which had never previously been considered as...
In this study, we assessed the main bioactive compounds of a broad apple germplasm collection, composed by 247 accessions wild (97) and domesticated (150) species. Among stilbenes, trans- cis-piceid were found to be ubiquitary components both cultivated apples. Apple was suggested second dietary source resveratrols. Results confirmed that selection pressure breeding domestication did not uniformly affect all phytochemicals contained in For instance, organic acids (malic ascorbic acid) some...
Olive pomace is a major waste product of olive oil production but remains rich in polyphenols and fibres. We measured the potential an pomace-enriched biscuit formulation delivering 17.1 ± 4.01 mg/100 g hydroxytyrosol its derivatives, to modulate composition metabolic activity human gut microbiota. In double-blind, controlled parallel dietary intervention 62 otherwise healthy hypercholesterolemic (total plasma cholesterol 180–240 mg/dl) subjects were randomly assigned eat 90 (olive-enriched...
Downy mildew (Plasmopara viticola) is one of the most destructive diseases cultivated species Vitis vinifera. The use resistant varieties, originally derived from backcrosses North American spp., a promising solution to reduce disease damage in vineyards. To shed light on type and timing pathogen-triggered resistance, this work aimed at discovering biomarkers for defense response variety Bianca, using leaf discs after infection with suspension P. viticola. We investigated primary secondary...
The development of new resistant varieties to the oomycete Plasmopara viticola (Berk.& Curt) is a promising way combat downy mildew (DM), one major diseases threatening cultivated grapevine (Vitis vinifera L.). Taking advantage segregating population derived from "Merzling" (a mid-resistant hybrid) and "Teroldego" susceptible landrace), 136 F1 individuals were characterized by combining genetic, phenotypic, gene expression data elucidate genetic basis DM resistance polyphenol biosynthesis...
Cutibacterium acnes (C. acnes) is recognized as one of the main triggers cutaneous inflammatory response in acne vulgaris, a chronic skin disorder with multifactorial origin. Witch hazel (Hamamelis virginiana L.) plant widely used for conditions, some preliminary anti-inflammatory evidence on skin, but lacking data conditions. This study aimed to evaluate effect glycolic extract from Hamamelis bark (HVE) versus C. acnes-induced inflammation human keratinocytes (HaCaT). Phytochemical...
The reduction of pesticide treatments is paramount importance for the sustainability viticulture, and it can be achieved through a combination strategies, including cultivation vines ( Vitis vinifera ) that are resistant or tolerant to diseases such as downy mildew (DM). In many crops, knock-out Downy Mildew Resistant 6 DMR6 proved successful in controlling DM-resistance, but effect mutations genes not yet known grapevine. Today, gene editing serves crop improvement with small specific while...
Citrus fruits are among the most economically important crops in world. In global market, peel is often considered a byproduct but substitutes an phenotypic characteristic of fruit and valuable source essential oils, flavonoids, carotenoids, phenolic acids with variable concentrations. The Mediterranean basin particularly dense area autochthonous genotypes that known for being healthy foods, which can be repertoires genes molecular breeding focus on plant resistance quality improvement....
Ellagitannins and ellagic acid conjugates are polyphenols present in the human diet, particular strawberries (Fragaria spp.). The first aim of this study was isolation structural characterization casuarictin 3-O-methyl 3'-O-α-rhamnopyranoside, which were found to be abundant Fragaria spp., along with agrimoniin. second accurate profiling quantification 26 ellagitannins six x ananassa cultivars two vesca species. third describe behavior during fruit ripening from green stage over-ripeness. It...
Botrytis cinerea is an important necrotroph in vineyards. Primary infections are mostly initiated by airborne conidia from overwintered sources around bloom, then the fungus remains quiescent bloom till maturity and egresses at ripeness. We previously described detail process of flower infection quiescence initiation. Here, we complete characterization studying cross-talk between two organismsplant during pathogen egression integrated transcriptomic metabolic analysis host pathogen. Flowers...
Juglans regia (L.) is cultivated worldwide for its nutrient-rich nuts. In Italy, despite the growing demand, walnut cultivation has gone through a strong decline in recent decades, which led to Italy being among top five net importing countries. To promote development of local high-quality Italian production, we devised multidisciplinary project highlight distinctive traits three varieties grown mountainous region Trentino (northeast Italy): heirloom 'Bleggiana', second accession called...