A5052733580- CRISPR and Genetic Engineering
- Insect-Plant Interactions and Control
- Plant and Fungal Interactions Research
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Insect Pheromone Research and Control
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Insect symbiosis and bacterial influences
- Neurobiology and Insect Physiology Research
- Insect and Pesticide Research
- Horticultural and Viticultural Research
- Plant and animal studies
- Phytoplasmas and Hemiptera pathogens
- Forest Insect Ecology and Management
- Insect behavior and control techniques
- Olfactory and Sensory Function Studies
- Plant Physiology and Cultivation Studies
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Plant biochemistry and biosynthesis
Fondazione Edmund Mach
2016-2023
Free University of Bozen-Bolzano
2016-2017
University of Padua
2017
Abstract Genome editing via CRISPR/Cas9 is a powerful technology, which has been widely applied to improve traits in cereals, vegetables and even fruit trees. For the delivery of components into dicotyledonous plants, Agrobacterium tumefaciens mediated gene transfer still prevalent method, although often accompanied by integration bacterial T-DNA host genome. We assessed two approaches order achieve excision from plant genome, minimizing extent foreign DNA left behind. The first based on...
The application of New Breeding Techniques (NBTs) in
The reduction of pesticide treatments is paramount importance for the sustainability viticulture, and it can be achieved through a combination strategies, including cultivation vines ( Vitis vinifera ) that are resistant or tolerant to diseases such as downy mildew (DM). In many crops, knock-out Downy Mildew Resistant 6 DMR6 proved successful in controlling DM-resistance, but effect mutations genes not yet known grapevine. Today, gene editing serves crop improvement with small specific while...
Abstract Olfaction plays a dominant role in the mate-finding and host selection behaviours of codling moth ( Cydia pomonella ), an important pest apple, pear walnut orchards worldwide. Antennal transcriptome analysis revealed number abundantly expressed genes related to olfactory system, including those encoding receptors (ORs) CpomOR1, CpomOR3 CpomOR6a, which belong pheromone receptor (PR) lineage, co-receptor (CpomOrco). Using heterologous expression, both Drosophila sensory neurones human...
Abstract Fire blight disease, caused by the bacterium Erwinia amylovora ( E. ), is responsible for substantial losses in cultivated apples worldwide. An important mechanism of plant immunity based on recognition conserved microbial molecules, named pathogen-associated or microbe-associated molecular patterns (PAMPs MAMPs), through pattern receptors (PRRs), leading to pattern-triggered (PTI). The interspecies transfer PRRs represents a promising strategy engineer broad-spectrum and durable...
Summary Herbivorous insects use olfactory cues to locate their host plant within a complex landscape. One such example is the European grapevine moth Lobesia botrana, key pest of grape in Palearctic region, which recently expanded both its geographical and range. Previous studies have showed that synthetic blend three terpenoids, ( E )‐β‐caryophyllene, )‐β‐farnesene )‐4,8‐dimethyl‐1,3,7‐nonatriene (DMNT), was as attractive for complete odour profile laboratory conditions. The same also...
Abstract CRISPR-Cas technology has widely extended the application fields of genome editing in plant breeding, making possible specific and minimal mutations within a genetic pool. With respect to standard technologies, machinery can be introduced form ribonucleoproteins (RNPs), thus avoiding introduction exogenous DNA into cells. The interest on DNA-free delivery cells is constantly increasing, especially case valuable woody plants elite varieties where CRISPR-Cas9 would preserve their...
Transient receptor potential (TRP) channels are an ancient family of cation channels, working as metabotropic triggers, which respond to physical and chemical environmental cues. Perception signals mediate reproductive behaviors is therefore important target for sustainable management tactics against the codling moth Cydia pomonella L. (Lepidoptera: Tortricidae). However, olfactory behavior strongly depends on diel periodicity correlation with cues, like temperature, cues thus essentially...
In vascular plants the cell-to-cell interactions coordinating morphogenetic and physiological processes are mediated, among others, by action of hormones, which also short mobile peptides were recognized to have roles as signals. Such peptide hormones (PHs) involved in defense responses, shoot root growth, meristem homeostasis, organ abscission, nutrient signaling, hormone crosstalk other developmental act both long distant ligands. this work, function CTG134, a peach gene encoding ROOT...
ABSTRACT The production and cultivation of vines ( Vitis vinifera ) tolerant or resistant to diseases such as downy mildew (DM) is a promising strategy reduce fungicides help viticulture sustainability. In many crops, generation knock-out mutants in host genes controlling susceptibility DM, Downy Mildew Resistant 6 DMR6 proven success obtain plants, while the effect mutations has yet be demonstrated grapevine. addition, small governing important traits can obtained by gene-editing...
Root chicory (Cichorium intybus L. var. sativum) is used to extract inulin, a fructose polymer as natural sweetener and prebiotic. However, bitter tasting sesquiterpene lactones, giving its known flavour, need be removed during inulin extraction. To avoid this extraction associated costs, recently variants with lower lactone content were created by inactivating the four copies of germacrene A synthase gene (CiGAS-S1, -S2, -S3, -L) which encode enzyme initiating biosynthesis in chicory. In...
The European grapevine moth, Lobesia botrana, is one of the most important pests grape. Feeding activity by caterpillars leads to direct damage reproductive plant tissues (flower buds and berries), also indirect promoting secondary infections microorganisms. Current control systems are based either on use insecticides or mating disruption: while first not environmentally friendly, second particularly suitable for non-delimited areas where pest population high. Previous studies have shown...
Abstract Agrobacterium tumefaciens- mediated gene transfer—actually the most used method to engineer plants—may lead integration of multiple copies T-DNA in plant genome, as well chimeric tissues composed modified cells and wild type cells. A molecular characterization transformed lines is thus a good practice select best ones for further investigation. Nowadays, several quantitative semi-quantitative techniques are available estimate copy number (CN) genetically plants. In this study, we...
Abstract Peach is a climacteric species whose ripening regulated by the plant hormone ethylene. A crosstalk mechanism with auxin necessary to support ethylene synthesis. The homeostasis control of also activity peptide hormones (PHs), acting both as short and long distant ligands. In this work, we investigated role CTG134 , peach gene encoding GOLVEN-like PH isolated in mesocarp at onset ripening. fruit, was expressed during transition its mRNA level induced 1-methylcyclopropene (1-MCP)...