A5043427910- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Genetically Modified Organisms Research
- CRISPR and Genetic Engineering
- Acoustic Wave Resonator Technologies
- Mycotoxins in Agriculture and Food
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
- Animal Disease Management and Epidemiology
- RNA Interference and Gene Delivery
- Identification and Quantification in Food
- Gas Sensing Nanomaterials and Sensors
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Coccidia and coccidiosis research
- Insect Resistance and Genetics
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana
2021-2024
National Agency for New Technologies, Energy and Sustainable Economic Development
2013-2015
University of Rome Tor Vergata
2014-2015
Screening strategies for GMO detection in food and feed are a crucial aspect testing laboratories streamlining the analytical workflow reducing turnaround time costs. These can be more or less complex even targeted according to ingredients product, but whatever choice, good basic approach is generally based on search 35S promoter (P35S), nos-terminator (T-nos) FMV (P-FMV). In this study, we compare singleplex real PCR method P35S, T-nos P-FMV currently adopted by Italian National Reference...
In this study, we have used a direct immunoassay where the simple binding between antigen and an antibody is detected.Immunoassays were performed in drop system, monitoring frequency decrease of quartz-crystal microbalance device because mass increasing during immunoreaction.The QCM sensor was coated on both sides by gold electrodes, only one side crystal (liquid side) contact with solution; other (contact always dry.We tested piezoelectric immunosensor for aflatoxin B1 (AFLA-B1) mycotoxin...
The act of selecting aptamers against blood serum leads to deep libraries oligonucleotide sequences that bind a range epitopes in blood. In this study we developed an enriched aptamer library by performing positive selection pool samples from transgenic mice (P301S) carrying the human tau gene and counter pooled negative segregant (wild type) mice. We demonstrated large proportion observed with next generation sequence (NGS) analysis were same round 5 6. As second step, applied aliquots 16...
This project focuses on the development of a DNA-aptamer based biosensor, which is referred to as an aptasensor, that would aid in detection mycotoxins foodstuffs. We developed aptamer biosensor for Aflatoxin B1 (AFB1), mycotoxin produced by Aspergillus flavus and parasiticus, species fungi. The characterization sensor aflatoxin was performed electrochemical impedance spectroscopy (EIS) cyclic voltammetry (CV). tested aptasensor AFB1 through immobilization DNA aptamers Poly (amido-amine)...
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Due to the increasing number of authorized events in European Union, it is crucial for official laboratories enforce market control detect and quantify genetically modified organisms. In this study, an in-house validation quantitative duplex ddPCR methods was performed involving MON87701, MON87769, MON89788 CV-127-9 assays with respect lectin reference gene. Since provide accurate quantification, show less sensitivity PCR inhibitors are more suitable multiplexing compared real-time PCR,...
Abstract Agrobacterium tumefaciens- mediated gene transfer—actually the most used method to engineer plants—may lead integration of multiple copies T-DNA in plant genome, as well chimeric tissues composed modified cells and wild type cells. A molecular characterization transformed lines is thus a good practice select best ones for further investigation. Nowadays, several quantitative semi-quantitative techniques are available estimate copy number (CN) genetically plants. In this study, we...
Abstract Background The analysis of genetically modified organisms (GMOs) in food and feed is broadly based on the detection target DNA sequences using PCR‐based methods. first step a typical workflow GMO testing laboratory extraction purification. Several methods have been described for this purpose so far. Whatever method adopted, yield quality extracted are essential factors success analysis. Results was variable between different methods, while efficiency appeared comparable. Each...