A5103012167- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Horticultural and Viticultural Research
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Plant biochemistry and biosynthesis
- Fungal Plant Pathogen Control
- Plant Physiology and Cultivation Studies
- RNA and protein synthesis mechanisms
- Plant Disease Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Phytoplasmas and Hemiptera pathogens
- Plant pathogens and resistance mechanisms
- Photosynthetic Processes and Mechanisms
- Banana Cultivation and Research
- Insect symbiosis and bacterial influences
- Nuts composition and effects
- Plant Gene Expression Analysis
- Genetically Modified Organisms Research
- Fermentation and Sensory Analysis
Fondazione Edmund Mach
2015-2024
Stockholm University
2020-2021
The bacterium Erwinia amylovora, the causal agent of fire blight disease in apple, triggers its infection through DspA/E effector which interacts with apple susceptibility protein MdDIPM4. In this work, MdDIPM4 knockout has been produced two Malus × domestica susceptible cultivars using CRISPR/Cas9 system delivered via Agrobacterium tumefaciens. Fifty-seven transgenic lines were screened to identify CRISPR/Cas9-induced mutations. An editing efficiency 75% was obtained. Seven edited a...
Abstract Genome editing via CRISPR/Cas9 is a powerful technology, which has been widely applied to improve traits in cereals, vegetables and even fruit trees. For the delivery of components into dicotyledonous plants, Agrobacterium tumefaciens mediated gene transfer still prevalent method, although often accompanied by integration bacterial T-DNA host genome. We assessed two approaches order achieve excision from plant genome, minimizing extent foreign DNA left behind. The first based on...
Epidermal Patterning Factor Like 9 (EPFL9), also known as STOMAGEN, is a cysteine-rich peptide that induces stomata formation in vascular plants, acting antagonistically to other epidermal patterning factors (EPF1, EPF2). In grapevine there are two EPFL9 genes, EPFL9-1 and EPFL9-2 sharing 82% identity at protein level the mature functional C-terminal domain. this study, CRISPR/Cas9 system was applied functionally characterize VvEPFL9-1 ‘Sugraone’, highly transformable genotype. A set of...
Breeding for resistance against the destructive fire blight disease of apples is most sustainable strategy to control menace this disease, and has become increasingly important in European apple breeding programs. Since cultivars are susceptible, wild accessions have been explored with quantitative trait loci detected a few species. Fire Malus fusca was described following phenotypic evaluations C-type strain Erwinia amylovora, Ea222_JKI, detection major QTL on chromosome 10 (Mfu10)...
Abstract Fire blight disease, caused by the bacterium Erwinia amylovora ( E. ), is responsible for substantial losses in cultivated apples worldwide. An important mechanism of plant immunity based on recognition conserved microbial molecules, named pathogen-associated or microbe-associated molecular patterns (PAMPs MAMPs), through pattern receptors (PRRs), leading to pattern-triggered (PTI). The interspecies transfer PRRs represents a promising strategy engineer broad-spectrum and durable...
Phloridzin is the most abundant polyphenolic compound in apple (Malus × domestica Borkh.), which results from action of a key phloretin-specific UDP-2'-O-glucosyltransferase (MdPGT1). Here, we simultaneously assessed effects targeting MdPGT1 by conventional transgenesis and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing. To this end, conducted transcriptomic metabolic analyses RNA interference knockdown...
Fire blight, a devastating disease caused by the bacterium Erwinia amylovora, is major threat to apple crop production. To improve our understanding of fire blight and identify potential strategies control pathogen, we studied protein HIPM (for HrpN-interacting from Malus spp.), which has previously been identified as interacting with E. amylovora effector HrpN. Transgenic plants were generated reduced expression, using an RNA interference construct, subsequently analyzed for susceptibility...
Abstract Pyramiding different fire blight resistance genes and QTLs in future apple cultivars is the most eco-friendly way to combat this disease. Identification of strong donors, introgression their into breeding material are a continuing effort programs. Thus, enormous been put research understand host – pathogen interactions mechanisms found Malus . The crabapple fusca (accession MAL0045) highly resistant blight, although strain-dependent, MAL0045 not overcome by any known strain Erwinia...
The sweet chestnut Castanea sativa Mill. is the only native species in Europe, and it a tree of high economic value that provides appreciated fruits valuable wood. In this study, we assembled high-quality nuclear genome ancient Italian variety 'Marrone di Chiusa Pesio' using combination Oxford Nanopore Technologies long reads, whole-genome Omni-C Illumina short reads.
Malus x domestica microRNA MdmiR285N is a potential key regulator of plant immunity, as it has been predicted to target 35 RNA transcripts coding for different disease resistance proteins involved in defense pathogens. In this study, the promoter region was isolated from apple genome and analyzed silico detect regulatory regions controlling its transcription. A complex network putative elements growth development, response hormones stress conditions, identified. Activity β-Glucoronidase...
Abstract Background A number of cellular processes have evolved in metazoans that increase the proteome repertoire relation to genome, such as alternative splicing and translation recoding. Another process, translational stop codon readthrough (SCR), generates C-terminally extended protein isoforms many eukaryotes, including yeast, plants, insects, humans. While comparative genome analyses predicted existence programmed SCR species humans, experimental proof its functional consequences are...
Abstract Fire blight disease, caused by the bacterium Erwinia amylovora ( E. ), is responsible for substantial losses in cultivated apple worldwide. An important mechanism of plant immunity based on recognition conserved microbial molecules, named pathogen- or microbe-associated molecular patterns (PAMPs MAMPs), through pattern receptors (PRRs), leading to pattern-triggered (PTI). The interspecies transfer PRRs represents a promising strategy engineer broad spectrum and durable disease...
Epidermal Patterning Factors are a class of cysteine rich peptides known to be involved in many developmental processes. The role EPF1, EPF2 and EPFL9 controlling leaf stomata formation has been well described model plants cereals, recently also grapevine, while little is about their activity other organs. aim our study investigate whether VviEPFL9-2 can have specific biological function grapevine roots, where it resulted expressed. As cultivated the form grafted plant, we focused on...
To enhance the breeding of new scab-resistant apple cultivars, a comprehensive understanding mechanisms governing major scab resistance genes is essential. Rvi12_Cd5 was previously identified as best candidate gene for Rvi12 crab "Hansen's baccata #2" by prediction and in silico analysis. In present study, used to transform scab-susceptible cultivar "Gala Galaxy." Two constructs were prepared: first carrying under control 35S promoter E9 terminator, second its native terminator. All...
Abstract Translational stop codon readthrough generates C-terminally extended protein isoforms. While evidence mounts of as a global phenomenon, proofs its functional consequences are scarce. We show that the mRNA for Drosophila POU/Oct transcription factor Drifter occurs at high rate and in spatiotemporal manner vivo , reaching above 50% prothoracic gland. Phylogenetic analyses suggested drifter is conserved among Dipterans, with C-terminal extensions harboring intrinsically disordered...
Abstract Agrobacterium tumefaciens- mediated gene transfer—actually the most used method to engineer plants—may lead integration of multiple copies T-DNA in plant genome, as well chimeric tissues composed modified cells and wild type cells. A molecular characterization transformed lines is thus a good practice select best ones for further investigation. Nowadays, several quantitative semi-quantitative techniques are available estimate copy number (CN) genetically plants. In this study, we...