A5020140482- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Bioenergy crop production and management
- Plant biochemistry and biosynthesis
- Mitochondrial Function and Pathology
- Antioxidant Activity and Oxidative Stress
- Plant Micronutrient Interactions and Effects
- Lichen and fungal ecology
- Horticultural and Viticultural Research
- Bryophyte Studies and Records
- Genetic diversity and population structure
- Plant Diversity and Evolution
- Plant Water Relations and Carbon Dynamics
- Chromosomal and Genetic Variations
- Plant Ecology and Taxonomy Studies
- Microbial Metabolic Engineering and Bioproduction
- Plant nutrient uptake and metabolism
- Botany and Plant Ecology Studies
- Seed Germination and Physiology
- Light effects on plants
- Plant Virus Research Studies
- Plant Taxonomy and Phylogenetics
- Fermentation and Sensory Analysis
Fondazione Edmund Mach
2016-2025
University of Palermo
2023-2025
University of Parma
2014
ETH Zurich
2014
University of Milano-Bicocca
2013
Max Planck Society
2000-2010
Max Planck Institute for Plant Breeding Research
2002-2010
Heinrich Heine University Düsseldorf
2001
University of Verona
1998-1999
Summary The mutants irt1‐1 and irt1‐2 of Arabidopsis thaliana were identified among a collection T‐DNA‐tagged lines on the basis decrease in effective quantum yield photosystem II. mutations responsible interfere with expression IRT1 , nuclear gene that encodes metal ion transporter IRT1. In irt1 mutants, photosensitivity chlorophyll fluorescence parameters, as well abundance composition photosynthetic apparatus, are significantly altered. Additional effects mutation under greenhouse...
The chromophore binding properties of the higher plant light-harvesting complex II have been studied by site-directed mutagenesis pigment-binding residues. Mutant apoproteins were overexpressed in Escherichia coli and then refoldedin vitro with purified chromophores to yield holoproteins selectively affected chlorophyll-binding sites. Biochemical spectroscopic characterization showed a specific loss pigments absorption spectral forms for each mutant, thus allowing identification bound most...
Drosophilid fruit flies have provided science with striking cases of behavioral adaptation and genetic innovation. A recent example is the invasive pest Drosophila suzukii, which, unlike most other Drosophila, lays eggs feeds on undamaged, ripening fruits. This not only poses a serious threat for cultivation but also offers an interesting model to study evolution We developed genome transcriptome resources D. suzukii. Coupling analyses these data field observations, we propose hypothesis...
Plastid genomes, also known as plastomes, are shaped by the selective forces acting on fundamental cellular functions they code for and thus expected to preserve signatures of adaptive path undertaken different plant species during evolution. To identify molecular positive selection associated adaptation contrasting ecological niches, we sequenced with Solexa technology plastomes two congeneric Brassicaceae habitat preference, Cardamine resedifolia impatiens.Following in-depth...
Summary In Arabidopsis thaliana , the D‐subunit of photosystem I (PSI‐D) is encoded by two functional genes, PsaD1 and PsaD2 which are highly homologous. Knock‐out alleles for each loci have been identified a combination forward reverse genetics. The double mutant psad1‐1 psad2‐1 seedling‐lethal, high‐chlorophyll‐fluorescent deficient all tested PSI subunits, indicating that PSI‐D essential photosynthesis. addition, plants show defect in accumulation thylakoid multiprotein complexes other...
Abstract With the aim of developing widely applicable gene markers for phylogenetic reconstructions at low taxonomic level, we tested copy nuclear Conserved Ortholog Set (COS) genes. Most 15 genes provided good amplification efficiency (as compared with rbcL ) from a set 67 representative angiosperm families. Nine selected COS were further characterized both intra‐ and interfamilial level on test set, including 25 species different While four led to incongruent results, remaining five...
Summary Aquilegia is a well‐known model system in the field of evolutionary biology, but obtaining resolved and well‐supported phylogenetic reconstruction for genus has been hindered by its recent rapid diversification. Here, we applied 454 next‐generation sequencing to PCR amplicons 21 most rapidly evolving regions plastome generate c . 24 kb sequences from each 84 individuals throughout genus. The resulting phylogeny resolution main lineages genus, although diversification such as European...
Epidermal Patterning Factor Like 9 (EPFL9), also known as STOMAGEN, is a cysteine-rich peptide that induces stomata formation in vascular plants, acting antagonistically to other epidermal patterning factors (EPF1, EPF2). In grapevine there are two EPFL9 genes, EPFL9-1 and EPFL9-2 sharing 82% identity at protein level the mature functional C-terminal domain. this study, CRISPR/Cas9 system was applied functionally characterize VvEPFL9-1 ‘Sugraone’, highly transformable genotype. A set of...
The localisation of the xanthophyll neoxanthin within structure major light harvesting complex (LHCII) higher plants has been investigated by site‐directed mutagenesis and spectroscopic methods. Mutation analysis performed on pigment binding sites in different helix domains leads to selective loss for mutations C thus localising this between A/B domains. Recombinant proteins two lutein molecules per polypeptide but lacking have used order determine contribution absorption linear dichroism...
We present an open‐access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of differentially expressed miRNAs in control as well treated library. The web‐platform is not restricted only NGS sequence dataset from animals potentially analyzes a wider for plants, viral supporting miRBase (version 19 193 species). can handle bloated amount read counts reports annotated microRNAs plant, animal datasets. isomiRex also provides estimation isomiRs, with higher copy...
Here, we report the surprising and, to our knowledge, unique example of horizontal interkingdom transfer a human opportunistic pathogen (Propionibacterium acnes) crop plant (the domesticated grapevine Vitis vinifera L.). Humans, like most organisms, have established long-lasting cohabitation with variety microbes, including pathogens and gut-associated bacteria. Studies which investigated dynamics such associations revealed numerous cases bacterial host switches from domestic animals humans....
Plants have evolved a set of mechanisms that control and respond to the uptake accumulation both essential non-essential metals, including chelation sequestration these elements by thiol ligands, such as glutathione phytochelatins. Indeed, peptides can chelate some quickly form thiol-metal complexes, segregate them in vacuolar compartment. Reasonably, conceptually similar be assumed responsible for transport metal complexes ̶ particular thiol-cadmium across plasma membrane, with consequent...
The funarioid moss species Physcomitrium eurystomum, which is threatened with extinction, was the subject of this study. riparian habitat type often under influence contaminated water, and, therefore, we tested selected potentially toxic elements (PTEs), namely zinc and copper, on development, physiological features, survival two different accessions (German Croatian). results obtained showed resilience to PTEs. Flow cytometry analyses revealed that differ significantly in terms genome size....
The prpl11-1 mutant of Arabidopsis thaliana was identified among a collection T-DNA tagged lines on the basis decrease in effective quantum yield photosystem II. mutation responsible localized to Prpl11, single-copy nuclear gene that encodes PRPL11, component large subunit plastid ribosome. amino acid sequence PRPL11 is very similar those L11 proteins from spinach and prokaryotes. In mutant, photosensitivity chlorophyll fluorescence parameters are significantly altered owing changes levels...
Summary The psae1‐1 mutant of Arabidopsis was identified on the basis a decrease in effective quantum yield photosystem II, among collection plants subjected to transposon tagging with Enhancer element. steady‐state redox level and rate re‐oxidation P700 are significantly altered mutants. responsible mutation localised psaE1 , one two genes that encode subunit E I. An additional allele, psae1‐2 by reverse genetics. In wild‐type plants, transcript is expressed at higher than psaE2 mRNA....
Abstract Photosystem I (PSI) of higher plants contains 18 subunits. Using Arabidopsis En insertion lines, we have isolated knockout alleles the genes psaG,psaH2, and psaK, which code for PSI-G, -H, -K. In mutants psak-1 andpsag-1.4, complete loss PSI-K -G, respectively, was confirmed, whereas residual H level in psah2-1.4is due to a second gene encoding PSI-H, psaH1. Double mutants, lacking also -K, or fraction together with three single were characterized their growth phenotypes PSI...
The genus Potentilla is closely related to that of Fragaria, the economically important strawberry genus. micrantha a species does not develop berries but shares numerous morphological and ecological characteristics with Fragaria vesca. These similarities make P. an attractive choice for comparative genomics studies F. vesca.In this study, genome was sequenced annotated, RNA-Seq data from different developmental stages flowering fruiting were used set gene predictions. A 327 Mbp sequence...
Physiological, biochemical and morpho-anatomical traits that determine the phenotypic plasticity of plants under drought were tested in two Arundinoideae with contrasting habitats, growth metabolism: fast-growing Arundo donax, which also is a strong isoprene emitter, slow-growing Hakonechloa macra does not invest on biosynthesis. In control conditions, A. donax displayed only higher photosynthesis but concentration carotenoids lower phenylpropanoid content than H. macra. drought-stressed...
Arundo donax L. (Poaceae) is considered one of the most promising energy crops in Mediterranean region because its high biomass yield and low input requirements, but to date no information on transcriptional responses water stress available.We obtained by Illumina-based RNA-seq whole root shoot transcriptomes young A. plants subjected osmotic/water with 10 20 % polyethylene glycol (PEG; 3 biological replicates/organ/condition corresponding 18 RNA-Seq libraries), identified a total 3034...
Abstract Monitoring biodiversity is of increasing importance in natural ecosystems. Metabarcoding can be used as a powerful molecular tool to complement traditional monitoring, total environmental DNA analyzed from complex samples containing different origin. The aim this research was demonstrate the potential pollen metabarcoding using chloroplast trn L partial gene sequencing characterize plant biodiversity. Collecting airborne biological particles with gravimetric Tauber traps four Natura...
The arsenic-specific ACR3 transporter plays pivotal roles in As detoxification yeast and a group of ancient tracheophytes, the ferns. Despite putative genes being present genomes bryophytes, whether they have same relevance also this lineage is currently unknown. In study, we characterized MpACR3 gene from bryophyte Marchantia polymorpha L. through multiplicity functional approaches ranging phylogenetic reconstruction, expression analysis, loss- gain-of-function as well genetic...