A5022375978- Enzyme Production and Characterization
- Phytase and its Applications
- Protein Hydrolysis and Bioactive Peptides
- Plant Micronutrient Interactions and Effects
- Marine and coastal plant biology
- Marine Biology and Ecology Research
- Insect Resistance and Genetics
- Food composition and properties
- Seed Germination and Physiology
- Meat and Animal Product Quality
- Peptidase Inhibition and Analysis
- Banana Cultivation and Research
- Plant tissue culture and regeneration
- Plant Physiology and Cultivation Studies
- Bryophyte Studies and Records
- Plant Genetic and Mutation Studies
- Food Allergy and Anaphylaxis Research
- Plant Stress Responses and Tolerance
- Coral and Marine Ecosystems Studies
- Nanoparticles: synthesis and applications
- Microplastics and Plastic Pollution
- Ocean Acidification Effects and Responses
- Lichen and fungal ecology
- Soybean genetics and cultivation
- Proteins in Food Systems
University of Pisa
2013-2024
CoNISMa
2017-2023
University of Catania
2005-2015
University of Verona
2015
Istituto Sperimentale per la Frutticoltura
2015
University of Wisconsin–Madison
2000-2003
United States Department of Agriculture
1998
Agricultural Research Service
1998
In the present work, we investigated response to Cd in Leptodictyum riparium, a cosmopolitan moss (Bryophyta) that can accumulate higher amounts of metals than other plants, even angiosperms, with absence or slight apparent damage. High-performance liquid chromatography followed by electrospray ionization tandem mass spectrometry extracts from L. riparium gametophytes, exposed 0, 36 and 360 µM for 7 days, revealed presence γ-glutamylcysteine (γ-EC), reduced glutathione (GSH), traces...
Plants have evolved a set of mechanisms that control and respond to the uptake accumulation both essential non-essential metals, including chelation sequestration these elements by thiol ligands, such as glutathione phytochelatins. Indeed, peptides can chelate some quickly form thiol-metal complexes, segregate them in vacuolar compartment. Reasonably, conceptually similar be assumed responsible for transport metal complexes ̶ particular thiol-cadmium across plasma membrane, with consequent...
Abstract Artificial light at night (ALAN) is one of the most recently recognized sources anthropogenic disturbance, with potentially severe effects on biological systems that are still to be fully explored. Among marine ecosystems, high‐shore habitats those more likely impacted by ALAN, due a intense exposition outdoor nocturnal lightings (mostly from lamps along coastal streets and promenades, or within harbours, ports marinas). By performing in situ manipulations direct source white LED...
The effects of climate change are likely to be dependent on local settings. Nonetheless, the compounded global and regional stressors remain poorly understood. Here, we used CO2 vents assess how ocean acidification seagrass, Posidonia oceanica, associated epiphytic community can modified by enhanced nutrient loading. P. oceanica at ambient low pH sites was exposed three levels for 16 months. response experimental conditions assessed combining analyses gene expression, plant growth,...
Flours of four pigmented (from orange to red and dark red) local Italian corns, studied for their soluble, soluble conjugate, insoluble-bound phenols flavonoids, showed a prevalence the fraction (70–80%). Correlations were found between flours antioxidant capacity, measured with CUPRAC, FRAP, DPPH methods, flavonoids content. A correlation was also ascorbic acid content power. Anthocyanins present in small amounts red/dark seeds; however, acid-alcohol assays spectral analyses pericarp...
Four co-eluting components, with experimentally measured M(r) of 23 658, 786, 24 278 and 406 Da, were detected by reversed-phase high-performance liquid chromatography (RP-HPLC) matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis in the dephosphorylated casein fraction a milk sample collected at middle lactation stage from an individual donkey belonging to Ragusano breed. By coupling RP-HPLC, two-dimensional polyacrylamide gel electrophoresis...
Although some charophytes (sister group to land plants) have been shown synthesize phytochelatins (PCs) in response cadmium (Cd), the functional characterization of their phytochelatin synthase (PCS) is still completely lacking. To investigate metal and presence PCS charophytes, we focused on species Nitella mucronata. A 40 kDa immunoreactive band was revealed mono-dimensional western blot by using a polyclonal antibody against Arabidopsis thaliana PCS1. In two-dimensional blot, putative...
The primary structure of four α(s2)-casein (CN) isoforms, present as minor components in the dephosphorylated CN fraction a milk sample collected Eastern Sicily from an individual donkey belonging to Ragusano breed at middle lactation stage, was determined, using known donkey's α(s2)-CN (GenBank Acc. No. CAV00691; M(r) 26,028 Da) reference. Proteins, with experimentally measured 25,429, 21,939, 25,203 and 21,713 Da, were isolated by combined use reversed-phase high-performance liquid...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 575:17-29 (2017) - DOI: https://doi.org/10.3354/meps12216 Spatio-temporal variability in Mediterranean rocky shore microphytobenthos E. Maggi1,2,*, L. Rindi1,2, M. Dal Bello1,2,5, D. Fontanini3, A. Capocchi3, Bongiorni4, Benedetti-Cecchi1,2 1Dipartimento di Biologia, Università Pisa, Via Derna 1, 56126 Italy...
Understanding how patterns and processes relate across spatial scales is one of the major goals in ecology. 1/ f models have been applied mostly to time series environmental ecological variables, but they can also be used analyse patterns. Since noise may display scale‐invariant behaviour, phenomena whose variability shows type scaling are susceptible further characterization using fractals or multifractals. Here we use spectral analysis multifractal techniques (generalized dimension...
The proteinases of germinating barley (Hordeum vulgare L.) hydrolyze storage proteins into amino acids and small peptides that can be used by the growing plant or, during brewing, yeast. They are critical for malting brewing processes because several aspects affected amounts protein, peptide, in wort. This study was carried out to quantitatively measure when endoproteinases form green malt whether they inactivated at high temperatures occur kilning. Little endoproteolytic activity present...
Abstract Enhanced persistence of stress sensitive species in harsh environments due to amelioration physical by habitat‐forming has been widely documented. By contrast, less attention given positive interactions that may enhance tolerance species, with cascading effects on biodiversity and ecosystem functioning. We used a combination field surveys experiments assess the epiphyte Jania rubens , its subtidal macroalgal host Halopithys incurva their associated invertebrate community, along...
It has been reported that germinated barley contains peptidases are sensitive to metal-chelating agents; however, none of these enzymes have isolated, nor their roles in investigated. Anion-exchange chromatography and chromatofocusing used isolate a group from (Hordeum vulgare cv. Morex) green malt agents. Their activities were studied using one- two-dimensional polyacrylamide gel electrophoresis. When analyzed on PAGE gels contained gelatin as substrate, the separated into three major...
There are great concerns for the accumulation in environment of small dimension plastics, such as micro- and nanoplastics. Due to their size, which facilitates uptake by organisms, nanoplastics particular concern. The toxic effects on plants already reported literature, however nothing is known, date, about possible climate change, increasing temperatures, toxicity plants. To address this issue, water fern Azolla filiculoides were grown at optimal (25 °C) or high (35 temperature, with...
Barley (Hordeum vulgare L.) malt contains endoproteinases belonging to all four of the commonly occurring classes, including serine proteinases. It also low molecular weight proteins that inhibit activities many these endoproteinases, but it had never been shown any barley or proteinases could be inhibited by endogenous proteins. is now reported some were concentrated using an "affinity" method activity a endoproteinase. Two-dimensional electrophoretic and in vitro analyses showed enzyme was...
Two citrus rootstocks, one sensitive to iron deficiency [Swingle Citrumelo (SC)] and the other tolerant [Carrizo Citrange, (CC)], were studied characterize variation in their root protein profile induced by iron-deficient conditions. Plants of both rootstocks grown two different soils, volcanic (v) calcareous (c), containing 0% 10% active lime, respectively. To evaluate effects soil on accumulation profiles (SCc vs SCv CCc CCv) characterized two-dimensional gel electrophoresis, thus...
Despite the effects of ocean acidification (OA) on seagrasses have been widely investigated, predictions seagrass performance under future climates need to consider multiple environmental factors. Here, we performed a mesocosm study assess OA shallow and deep Posidonia oceanica plants. The experiment was run in 2021 repeated 2022, year characterized by prolonged warm water event, test how plants are modulated thermal stress. response P. experimental conditions investigated at different...