A5089813198- Thyroid Disorders and Treatments
- Hormonal and reproductive studies
- Vitamin D Research Studies
- Advanced Chemical Sensor Technologies
- Analytical chemistry methods development
- Mass Spectrometry Techniques and Applications
- Nitric Oxide and Endothelin Effects
- Edible Oils Quality and Analysis
- Cancer, Hypoxia, and Metabolism
- Hormonal Regulation and Hypertension
- Metabolism, Diabetes, and Cancer
- Adrenal Hormones and Disorders
- Analytical Methods in Pharmaceuticals
- Growth Hormone and Insulin-like Growth Factors
- Sexual Differentiation and Disorders
- Ion channel regulation and function
- Analytical Chemistry and Chromatography
- Pharmacological Effects and Assays
- Plant Stress Responses and Tolerance
- Inflammatory mediators and NSAID effects
- Neonatal Health and Biochemistry
- Diabetes Treatment and Management
- Phytochemicals and Antioxidant Activities
- Stress Responses and Cortisol
- Mitochondrial Function and Pathology
University of Pisa
2015-2024
Aga Khan University Hospital
2024
Azienda Ospedaliera Universitaria Pisana
2018-2023
Ospedale Santa Chiara
2017-2023
Ospedale Sant Antonio
2020
The University of Queensland
2015
Ambiente Italia (Italy)
2011
University of Milan
2006
National Research Council
2006
Institute for the Chemistry of OrganoMetallic Compounds
2005
Introduction Short-chain fatty acids (SCFAs) are the main by-products of microbial fermentations occurring in human intestine and directly involved host’s physiological balance. As impaired gut concentrations acetic, propionic, butyric often associated with systemic disorders, administration SCFA-producing microorganisms has been suggested as attractive approach to solve symptoms related SCFA deficiency. Methods In this research, nine probiotic strains ( Bacillus clausii NR, OC, SIN, T,...
Agrifood waste products are often considered rich sources of bioactive compounds that can be conveniently recovered. Due to these peculiar characteristics, the study is attracting great interest in nutraceutical research. Olive mill wastewaters (OMWWs) generated by extra virgin olive oil (EVOO) production, and they pose environmental challenges due their disposal. This aimed characterize polyphenolic profile evaluate properties OMWW extracts from two Tuscan cultivars, Leccino (CL) Frantoio...
3-Iodothyronamine (T1AM) is a novel relative of thyroid hormone, able to interact with specific G protein-coupled receptors, known as trace amine-associated receptors. Significant functional effects are produced by exogenous T1AM, including negative inotropic and chronotropic effect in cardiac preparations. This work was aimed at estimating endogenous T1AM concentration different tissues determining its metabolism. A HPLC tandem mass spectrometry assay developed, allowing detection...
Abstract Similarities in cardiac gene expression hypothyroidism and left ventricular (LV) pathological remodeling after myocardial infarction (MI) suggest a role for impaired thyroid hormone (TH) signaling the development of heart failure. Increased activity TH-degrading enzyme type 3 deiodinase (D3) is recognized as potential cause. In present study, we investigated D3 over an 8-wk period MI C57Bl/6J mice. Pathological noninfarcted part LV was evident from cardiomyocyte hypertrophy,...
Oleocanthal is one of the phenolic compounds extra virgin olive oil with important anti-inflammatory properties. Although its potential anticancer activity has been reported, only limited evidence provided in cutaneous malignant melanoma. The present study aimed at investigating selective vitro antiproliferative oleocanthal against human melanoma cells. Since not commercially available, it was obtained as a pure standard by direct extraction and purification from oil. Cell viability...
Background Hypovitaminosis D has been suggested to play a possible role in coronavirus disease 2019 (COVID-19) infection. Methods The aim of this study is analyze the relationship between vitamin status and biochemical panel inflammatory markers cohort patients with COVID-19. A secondary endpoint was evaluate correlation 25OHD levels severity disease. Ninety-three consecutive COVID-19-related pneumonia were evaluated from March May 2020 two hospital units Pisa, whom markers, levels, P/F...
3-Iodothyronamine (T1AM), an endogenous derivative of thyroid hormones, is regarded as a rapid modulator behaviour and metabolism. To determine whether brain hormone levels contribute to these effects, we investigated the effect central administration T1AM on learning pain threshold mice either untreated or pretreated with clorgyline (2.5 mg·kg(-1) , i.p.), inhibitor amine oxidative metabolism.T1AM (0.13, 0.4, 1.32 4 μg·kg(-1) ) vehicle was injected i.c.v. into male mice, after 30 min their...
Thyroid dysfunction is common in individuals with diabetes mellitus (DM) and may contribute to the associated cardiac dysfunction. However, little known about extent pathophysiological consequences of low thyroid conditions on heart DM. DM was induced adult female Sprague Dawley (SD) rats by injection nicotinamide (N; 200 mg/kg) followed streptozotocin (STZ; 65 mg/kg). One month after STZ/N, were randomized following groups (N = 10/group): STZ/N or + 0.03 µg/mL T3; age-matched...
(1) Background: In recent years, numerous studies have highlighted the beneficial effects of extra virgin olive oil (EVOO) as an active ingredient against chronic diseases. The properties EVOO are due to its peculiar composition, mainly rich content polyphenols. fact, polyphenols may contribute counteract oxidative stress, which often accompanies this work, antioxidant high-value polyphenol oleocanthal (OC) and main metabolites, tyrosol (Tyr) oleocanthalic acid (OA), respectively, been...
Plants have evolved a set of mechanisms that control and respond to the uptake accumulation both essential non-essential metals, including chelation sequestration these elements by thiol ligands, such as glutathione phytochelatins. Indeed, peptides can chelate some quickly form thiol-metal complexes, segregate them in vacuolar compartment. Reasonably, conceptually similar be assumed responsible for transport metal complexes ̶ particular thiol-cadmium across plasma membrane, with consequent...
New Psychoactive Substances is one of the biggest challenges for forensic toxicology. Artificially manufactured drugs that are aimed at mimicking effects substances under legal regulations commonly present unique regarding identification and detection
Context and Objective: The primary purpose of this study was to detect quantify 3-iodothyronamine (T1AM), an endogenous biogenic amine related thyroid hormone, in human blood. Design: T1AM, total T3, T4 were assayed serum by a novel HPLC tandem mass spectrometry assay, which has already been validated animal investigations, the results standard clinical laboratory variables. Setting Patients: series included one healthy volunteer, 24 patients admitted cardiological ward, 17 ambulatory...
BACKGROUND AND PURPOSE Preclinical pharmacology of 3-iodothyronamine (T1AM), an endogenous derivative thyroid hormones, indicates that it is a rapid modulator rodent metabolism and behaviour. Since T1AM undergoes enzymatic degradation, particularly by MAO, we hypothesized the effects might be altered inhibition MAO. EXPERIMENTAL APPROACH We investigated injecting (i.c.v.) on (i) feeding behaviour, hyperglycaemia plasma levels hormones (ii) systemic bioavailability, in overnight fasted mice,...
3-Iodothyroacetic acid (TA1) is an end product of thyroid hormone metabolism. So far, it not known if TA1 present in mouse brain and has any pharmacological effects.TA1 levels were measured by HPLC coupled to mass spectrometry. After i.c.v. administration exogenous (0.4, 1.32 4 μg·kg(-1) ) mice, memory acquisition-retention (passive avoidance paradigm with a light-dark box), pain threshold thermal stimulus (51.5°C; hot plate test) plasma glucose (glucorefractometer) evaluated. Similar assays...
At the time of clinical onset type 1 diabetes (T1D), we investigated 82 pediatric cases in parallel with 117 non-diabetic controls matched by age, geographic area, and collection. The occurrence an enteroviral infection was evaluated peripheral blood using a sensitive method capable detecting virtually all human enterovirus (EV) types. While were consistently EV-negative, 65% T1D carried EVs blood. vitamin D status assessed measuring concentration 25-hydroxyvitamin [25(OH)D] serum. Levels...
Abstract 3-Iodothyronamine (T1AM) is an endogenous thyroid hormone derivative with unknown biosynthetic origins. Structural similarities have led to the hypothesis that T1AM extrathyroidal metabolite of T4. This study uses isotope-labeled T4 [heavy-T4 (H-T4)] can be distinguished from by mass spectrometry, which allows metabolites identified based on presence this unique isotope signature. Endogenous levels depend upon status and decrease induction hypothyroidism. However, in hypothyroid...
Abstract Aims/hypothesis The glucosuria induced by sodium–glucose cotransporter 2 (SGLT2) inhibition stimulates endogenous (hepatic) glucose production (EGP), blunting the decline in HbA 1c . We hypothesised that, response to glucosuria, a renal signal is generated and EGP. To examine effect of acute administration SGLT2 inhibitors on EGP, we studied non-diabetic individuals who had undergone transplant with without removal native kidneys. Methods This was parallel, randomised, double-blind,...
The human retinal pigment epithelium-specific 65-kDa protein (hRPE65) plays a crucial role within the retinoid visual cycle and several mutations affecting either its expression level or enzymatic function are associated with inherited diseases such as Retinitis Pigmentosa. gene therapy product voretigene neparvovec (Luxturna) has been recently approved for treating hereditary dystrophies; however, treatment is currently accessible only to patients presenting confirmed biallelic that...