A5029868998- Identification and Quantification in Food
- Meat and Animal Product Quality
- Calpain Protease Function and Regulation
- Aquaculture disease management and microbiota
- Food Supply Chain Traceability
- Isotope Analysis in Ecology
- Parasite Biology and Host Interactions
- Fish Ecology and Management Studies
- Marine and fisheries research
- Vibrio bacteria research studies
- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Muscle metabolism and nutrition
- Protein Hydrolysis and Bioactive Peptides
- Myxozoan Parasites in Aquatic Species
- Molecular Biology Techniques and Applications
- Marine Bivalve and Aquaculture Studies
- Biochemical effects in animals
- Genetic diversity and population structure
- Food Industry and Aquatic Biology
- Environmental DNA in Biodiversity Studies
- Sports Performance and Training
- Ichthyology and Marine Biology
- Glycosylation and Glycoproteins Research
- Marine Ecology and Invasive Species
Ifremer
2014-2024
Laboratoire des Sciences de l’Information et de la Communication
2012
Joint Research Centre
2008
École Nationale d'Ingénieurs des Techniques des Industries Agro-Alimentaires
2005
Centre National de la Recherche Scientifique
2005
Institut National Polytechnique de Toulouse
2005
University College Cork
2000
Illegal, Unreported and Unregulated fishing has had a major role in the overexploitation of global fish populations. In response, international regulations have been imposed many fisheries 'eco-certified' by consumer organizations, but methods for independent control catch certificates eco-labels are urgently needed. Here we show that, using gene-associated single nucleotide polymorphisms, individual marine can be assigned back to population origin with unprecedented high levels precision....
Abstract This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according their mitochondrial cytochrome b nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine comprising the main Actinopterygii family groups. When used phylogenetic analyses, its combination two genes with different evolutionary rates serves identify at level. We provide flow diagram...
ABSTRACT High pressure is an innovative non‐thermal food‐preservation technology. We studied the effect of high‐pressure treatment up to 500 MPa for 5 min on physical characteristics sea bass fillets after 0, 7, and 14 d refrigerated storage. Color results exhibited increase lightness a slight change hue, which might be imperceptible in cooked fish. High‐pressure induced decrease exudation water‐holding capacity. Pressure above 300 provoked higher fish hardness storage than untreated sample,...
Tuna fisheries and processing represent economic activities of paramount importance around the world. Most these products are traded for human consumption in general highly demanded commodities. However, not all tuna achieve same market price, some consumers willing to pay a huge amount money certain species (i.e. Japanese Bluefin tuna) while other rather affordable Skipjack tuna), therefore mislabelling has been observed frequently. We collected analysed 545 samples six European countries,...
Cryptosporidium, a zoonotic pathogen, is able to infect wide range of hosts including wild and domestic animals, humans. Although it well known that some parasites are both fish pathogens recognized agents zoonosis with public health impact, little information available concerning the prevalence Cryptosporidium in aquatic environments. To evaluate spp. commercially important edible marine different European seas (English channel, North sea, Bay Biscay, Celtic sea Mediterranean sea), 1,853...
A direct sequencing method based on a 103 bp diagnostic sequence derived from species-specific mitochondrial DNA cytochrome b of 150 obtained by Polymerase Chain Reaction was tested for the identification 47 commercial canned sardine and sardine-type products various countries. Multiple alignment 14 analyzed reference samples belonging to Clupeomorpha species performed versus samples. Low intraspecific variability observed (≤0.03), whereas mean interspecific 0.23. phylogenetic tree...
This study was devoted to the identification of specific peptides and proteins which can be used as indicators freshness in fish. The post mortem evolution protein patterns farmed sea bass muscle monitored by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) two-dimensional (2-DE) after 0, 2, 4, 6 days cold storage. SDS-electrophoresis, total soluble low-ionic-strength solutions, revealed gradual disappearance a band 16 kDa immediately fish death. 2-DE allowed...
α-Actinin release and its degradation from myofibrils Z-line were studied in post mortemwhite dorsal muscle bass sea trout stored at 4°C 10°C. Using α-actinin specific antibodies, we show that this protein is rapidly released within the first 24 h for two species, reaches a plateau 4 days. Proteolysis take place very yielding 80 40 kDa fragments as major bands of proteolysis. Sea more resistant, not significantly degraded even 10 days after death. In case 10°C, an increasing quantity...
Traceability in the fish food sector plays an increasingly important role for consumer protection and confidence building. This is reflected by introduction of legislation rules covering traceability on national international levels. Although through labeling well established supported respective regulations, monitoring enforcement these are still hampered lack efficient diagnostic tools. We describe protocols using a direct sequencing method based 212-274-bp sequences derived from...
Bigeye tuna (Thunnus obesus) and yellowfin albacares) are among the most widely used species for canning purposes. Not only substitution but also mixing of is prohibited by European regulation canned products. However, as juveniles bigeye tunas very difficult to distinguish, unintentional substitutions may occur during process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 cytochrome c oxidase II genes were identify tuna, respectively, utilizing TaqMan qPCR...
ABSTRACT The degradation of desmin was studied in postmortem white dorsal muscle from sea bass, brown trout, turbot and sardine, stored at 0–4°C. Based on specific antibody tests, results showed that the extent rate fish varied considerably among species. There no during first 4 days storage bass whereas 10 to 20% degraded turbot. Sardine presented a very complex pattern products aggregated fragments detected within 24h. Most polypeptides were larger than nondenatured desmin. Desmin would...
Vibrio parahaemolyticus is the leading bacterial cause of gastroenteritis associated with seafood consumption worldwide. Not all members species are thought to be pathogenic, thus identification virulent organisms essential protect public health and industry. Correlations human disease known genetic markers (e.g. thermostable direct hemolysin (TDH), TDH-related (TRH)) appear complex. Some isolates recovered from patients lack these factors, while their presence has become increasingly noted...
High-pressure processing is a nonthermal technique ensuring food product safety and enabling longer shelf life. The purpose of this experiment was to evaluate the effect high pressure on main proteolytic enzymes involved in fish muscle degradation during storage. Enzymes were extracted with sarcoplasmic proteins from Dicentrarchus labrax sea bass white muscle. Activity cathepsins B, D, H, L quantified protein extract, whereas calpain activity evaluated after isolation its endogenous...
Abstract The effects of m‐calpain isolated from the skeletal muscle sea bass on sarcoplasmic and myofibrillar proteins same tissue were examined in vitro . Incubation with resulted only a slight decrease (0.7 kDa) molecular weight (MW) 26.5 kDa protein. Degradation myofibrils, monitored by quantification TCA‐soluble peptides generated, maximum amount being generated after 1 h incubation at 25 °C. Noticeable modifications SDS‐PAGE profile digested myofibrils observed, including partial...
Two-dimensional electrophoresis was used to study proteolysis in salmon fillets inoculated or not with the starter culture Lactobacillus sake LAD. Protein fragments appeared increasingly time both samples, indicating that main quantitative changes were due endogenous enzymes. In most acidic zone (pI = 4-6. 20) particularly, overall independent from processing. contrast, fermentation had a significant effect pH range 6.20-8.35, suggesting specificity of bacterial proteases L. toward alkaline...