A5001302202- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Metal complexes synthesis and properties
- Photosynthetic Processes and Mechanisms
- Lanthanide and Transition Metal Complexes
- X-ray Diffraction in Crystallography
- Surfactants and Colloidal Systems
- Crystallization and Solubility Studies
- Spectroscopy and Quantum Chemical Studies
- Cellular transport and secretion
- Force Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Nanopore and Nanochannel Transport Studies
- Protein Interaction Studies and Fluorescence Analysis
- Lipid metabolism and biosynthesis
- Lysosomal Storage Disorders Research
- Drug Transport and Resistance Mechanisms
- Erythrocyte Function and Pathophysiology
- Cholesterol and Lipid Metabolism
- RNA Interference and Gene Delivery
- Metabolomics and Mass Spectrometry Studies
- Synthesis and biological activity
- Essential Oils and Antimicrobial Activity
- Protein Structure and Dynamics
- Synthesis and Biological Evaluation
University of Lisbon
2015-2024
Rede de Química e Tecnologia
2010-2022
Instituto Politécnico de Lisboa
2003-2020
Universidade Federal de Viçosa
2020
Universidade Tiradentes
2020
Faculdade de Tecnologia e Ciências
2015
Universidade Federal do Paraná
2012
Sport Lisboa e Benfica
2009-2010
Utrecht University
2008
Instituto Superior Técnico
2001-2007
The mechanism of action 2-hydroxyoleic acid (2OHOA), a potent antitumor compound, has not yet been fully elucidated. Here, we show that human cancer cells have markedly lower levels sphingomyelin (SM) than nontumor (MRC-5) cells. In this context, 2OHOA treatment strongly augments SM mass (4.6-fold), restoring the found in MRC-5 cells, while loss phosphatidylethanolamine and phosphatidylcholine is observed (57 30%, respectively). increased was due to rapid highly specific activation synthases...
To understand the formation and properties of ceramide-enriched domains in cell membranes, biophysical binary system palmitoyloleoylphosphatidylcholine (POPC)/palmitoylceramide were thoroughly characterized. Diverse fluorescent probes parameters necessary to unravel complexity this apparently simple system. For first time, a complete phase diagram is reported, characterizing lamellar phases these mixtures, providing quantitative framework integrating biological studies. The suggests that...
The plasma membrane of Saccharomyces cerevisiae was studied using the probes trans-parinaric acid and diphenylhexatriene. Diphenylhexatriene anisotropy is a good reporter global order. fluorescence lifetimes are particularly sensitive to presence nature ordered domains, but thus far they have not been measured in yeast cells. A long lifetime typical gel phase (>30 ns) found wild-type (WT) cells from two different genetic backgrounds, at 24 30 °C, providing first direct evidence for domains...
Four complexes combining the {Ru(p-cym)} moiety (p-cym = para-cymene) with thiosemicarbazone (TSC) ligands containing 5-nitrofuryl pharmacophore were investigated in vitro for their properties as prospective anti-tumour agents. The compounds are dimeric structures of general formula [Ru2(p-cym)2(L)2]X2 where X Cl(-), PF6(-) and L deprotonated 5-nitrofuraldehyde TSC (L1), N-methyl (L2), N-ethyl (L3) N-phenyl (L4) derivatives. precursor [RuCl2(p-cym)]2, all L1-L4and corresponding 1-4 screened...
Influenza virus hemagglutinin (HA) has been suggested to be enriched in liquid-ordered lipid domains named rafts, which represent an important step assembly. We employed Förster resonance energy transfer (FRET) via fluorescence lifetime imaging microscopy study the interaction of cytoplasmic and transmembrane domain (TMD) HA with agly co sylphos pha tidyl ino si tol (GPI)-anchored peptide, established marker for rafts exoplasmic leaflet living mammalian plasma membranes. Cyan fluorescent...
In this paper we present a first insight into the cytotoxic action mode of copper(I) iodide or thiocyanate complexes with phosphine derivative sparfloxacin (a 3rd generation fluoroquinolone antibiotic agent) and 2,9-dimethyl-1,10-phenanthroline 2,2'-biquinoline as auxiliary ligands. The in vitro activity new was tested against two cancer cell lines (CT26--mouse colon carcinoma A549--human lung adenocarcinoma). An ICP-MS study revealed marked time-dependent intracellular copper accumulation...
Anthrax is an infectious disease caused by Bacillus anthracis, a bioterrorism agent that develops resistance to clinically used antibiotics. Therefore, alternative mechanisms of action remain challenge. Herein, we disclose deoxy glycosides responsible for specific carbohydrate-phospholipid interactions, causing phosphatidylethanolamine lamellar-to-inverted hexagonal phase transition and acting over B. anthracis cereus as potent selective bactericides. Biological studies the synthesized...
To date, it is widely accepted that microdomains do form in the biological membranes of all eukaryotic cells, and quite possibly also prokaryotes. Those sub-micrometric domains play crucial roles signaling, intracellular transport, even inter-cellular communications. Despite their ubiquitous distribution, broad lasting interest invested those microdomains, actual nature composition, physical rules regiment assembly still remain elusive hotly debated. One most often considered models raft...
Nystatin-induced membrane permeabilization is related to its effects on properties and organization.
Quercetin impact on lipid bilayers suggests a dual action mechanism at cell membranes. This widespread polyphenol displays high partition with low interference in the more fluid membrane domains, vulnerable to oxidative attack, but strong perturbation of cholesterol/sphingolipid enriched where signalling platforms are expected assemble.